Amplified electronic transduction of oligonucleotide interactions: novel routes for Tay–Sachs biosensors

Abstract Two different configurations for the amplified microgravimetric analysis of oligonucleotides (DNA) are presented. A thiol-tagged oligonucleotide, ( 1 ), is assembled on a Au-quartz electrode, and the complementary oligonucleotide ( 2 ) is microgravimetrically sensed by the crystal. Formation of the ds-oligonucleotide assembly is amplified using mouse anti-dsDNA antibody and goat anti-mouse Fc antibody. Alternatively, a biotin-labeled oligonucleotide, ( 4 ), is used to form the ds-oligonucleotide. Biotin is used to amplify the formation of the ds-complex. An electrochemical method, using cytochrome c as redox probe, to assay the formation of the ds-oligonucleotide assembly, is described. The 5′-carboxylic acid-functionalized oligonucleotide ( 5 ) is coupled to a cystamine monolayer associated with an Au-electrode. The electrostatic attraction of cytochrome c to the ds-oligonucleotide complex facilitates the interfacial electron transfer to cytochrome c . Formation of the ds-oligonucleotide assembly is detected electrochemically using differential pulse voltammetry.

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