Genetic characterization of lentil genotypes based on SDS-PAGE

Lentil (Lens culinaris Medic) is a very important pulse crop. Fourteen Lentil genotypes namely; PL-4, KLS-218, KLS-320, L4147, K-75, KLB-08-4, KLS-09-3, VL-126, JL-1, L84-8, PL-5, KLB-303, IPL-81, DPL-62 were characterized on the basis of protein profiling through SDS-PAGE. Banding patterns through SDS-PAGE, found that the number of protein bands found in 14 genotypes ranged from 12 to 20 with Rm value 0.07 to 0.93 for Tris soluble proteins. UPGMA cluster analysis all the genotypes fall in seven cluster groups. SDS-PAGE for tris soluble proteins found suitable for testing distinctness, uniformity, stability of varieties for registration and identification. Further, characterization and identification of genotypes of lentil, on the basis of their protein electrophoretic profile through SDSPAGE was resulted distinct banding pattern and act as ‘genotypic finger printing’.

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