Chromosome preparation and analysis of early chorionic villus cells in 116 cases

Objective: To explore the value of chromosomal preparation of chorionic villus cells conducted by cultivation method in prenatal diagnosis and spontaneous abortion.Methods: Chromosome preparation and analysis of early chorionic villus cells in 21 samples of prenatal diagnosis and 95 cases of spontaneous abortion.Results: Among prenatal diagnosis cases,chorionic villus was successfully cultured in 20 cases(95.2%) and five abnormal embryo karyotypes were identified.Of other cases of spontaneous abortion,the successful cell culture rate was 92.6%,abnormal chromosome detection rate was 48.9%,antosomal triploids and X monosomy were common.Conclusion: The method of culture and chromosome preparation of villis is stable and reliable.It is feasible to use these methods in the prenatal cytogenetic diagnose and detection the reason of spontaneous abortion.