Immobilization of Genetically-Modified d-Amino Acid Oxidase and Catalase on Carbon Nanotubes to Improve the Catalytic Efficiency

d-amino acid oxidase (DAAO) and catalase (CAT) have been genetically modified by fusing them to an elastin-like polypeptide (ELP). ELP-DAAO and ELP-CAT have been separately immobilized on multi-walled carbon nanotubes (MWNTs). It has been found that the secondary structures of the enzymes have been preserved. ELP-DAAO catalyzed the oxidative deamination of d-alanine, and H2O2 was evolved continuously. When the MWNT-supported enzymes were used together, the generated hydrogen peroxide of ELP-DAAO could be decomposed in situ. The catalytic efficiency of the two immobilized enzymes was more than five times greater than that of free ELP-DAAO when the ratio of immobilized ELP-CAT to immobilized ELP-DAAO was larger than 1:1.

[1]  Wei Feng,et al.  Enhancement of the solubility and stability of D-amino acid oxidase by fusion to an elastin like polypeptide. , 2015, Journal of biotechnology.

[2]  Wei Feng,et al.  Specific immobilization of D-amino acid oxidase on hematin-functionalized support mimicking multi-enzyme catalysis , 2015 .

[3]  Shouji Takahashi,et al.  A Highly Stable d-Amino Acid Oxidase of the Thermophilic Bacterium Rubrobacter xylanophilus , 2014, Applied and Environmental Microbiology.

[4]  Wei Feng,et al.  Sodium hexadecyl sulfate as an interfacial substance adjusting the adsorption of a protein on carbon nanotubes. , 2014, ACS applied materials & interfaces.

[5]  J. Qiao,et al.  Construction of a D-amino acid oxidase reactor based on magnetic nanoparticles modified by a reactive polymer and its application in screening enzyme inhibitors. , 2014, ACS applied materials & interfaces.

[6]  Wei Feng,et al.  Interaction of Ionic Liquid [bmin][CF3SO3] with Lysozyme Investigated by Two-Dimensional Fourier Transform Infrared Spectroscopy , 2014 .

[7]  Yasuhisa Asano,et al.  Tailoring D-amino acid oxidase from the pig kidney to R-stereoselective amine oxidase and its use in the deracemization of α-methylbenzylamine. , 2014, Angewandte Chemie.

[8]  Yingchun Zhu,et al.  Manageable cytotoxicity of nanocapsules immobilizing D-amino acid oxidase via exogenous administration of nontoxic prodrug , 2014 .

[9]  D. Wei,et al.  High-level soluble and functional expression of Trigonopsis variabilisd-amino acid oxidase in Escherichia coli , 2014, Bioprocess and Biosystems Engineering.

[10]  M. Yoshimoto,et al.  Oligolamellar vesicles for covalent immobilization and stabilization of D-amino acid oxidase. , 2013, Enzyme and microbial technology.

[11]  Á. Berenguer-Murcia,et al.  Hydrogen Peroxide in Biocatalysis. A Dangerous Liaison , 2012 .

[12]  B. Nidetzky,et al.  Oriented and selective enzyme immobilization on functionalized silica carrier using the cationic binding module Zbasic2: Design of a heterogeneous D‐amino acid oxidase catalyst on porous glass , 2012, Biotechnology and bioengineering.

[13]  Byung-Gee Kim,et al.  Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and ω-transaminase. , 2012, Organic & biomolecular chemistry.

[14]  W. Fong,et al.  Entrapment of a Trigonopsis variabilis D‐amino acid oxidase variant F54Y for oxidative deamination of cephalosporin C , 2011 .

[15]  Loredano Pollegioni,et al.  New biotech applications from evolved D-amino acid oxidases. , 2011, Trends in biotechnology.

[16]  Ramesh N. Patel,et al.  Enzymatic Preparation of an (S)-Amino Acid from a Racemic Amino Acid , 2011 .

[17]  Wei Feng,et al.  Lipase Covalently Attached to Multiwalled Carbon Nanotubes as an Efficient Catalyst in Organic Solvent , 2010 .

[18]  Desiree Pressnitz,et al.  Deracemization of mexiletine biocatalyzed by omega-transaminases. , 2009, Organic letters.

[19]  Carlos D. Garcia,et al.  Interaction of D-amino acid oxidase with carbon nanotubes: implications in the design of biosensors. , 2009, Analytical chemistry.

[20]  L. Pollegioni,et al.  A biosensor for all D-amino acids using evolved D-amino acid oxidase. , 2008, Journal of biotechnology.

[21]  Loredano Pollegioni,et al.  Characterization of a yeast D-amino acid oxidase microbiosensor for D-serine detection in the central nervous system. , 2007, Analytical chemistry.

[22]  R. Fernández-Lafuente,et al.  Enzyme stabilization by glutaraldehyde crosslinking of adsorbed proteins on aminated supports. , 2005, Journal of biotechnology.

[23]  N. Kotov,et al.  Aqueous dispersions of single-wall and multiwall carbon nanotubes with designed amphiphilic polycations. , 2005, Journal of the American Chemical Society.

[24]  Chiaki Imada,et al.  Development of a D-alanine sensor for the monitoring of a fermentation using the improved selectivity by the combination of D-amino acid oxidase and pyruvate oxidase. , 2003, Biosensors & bioelectronics.

[25]  L. Fischer,et al.  Minimization of by-product formation during d-amino acid oxidase catalyzed racemate resolution of d/l-amino acids , 2002 .

[26]  Ashutosh Chilkoti,et al.  Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide system. , 2002, Biomacromolecules.

[27]  J. Pingarrón,et al.  Chiral analysis of amino acids using electrochemical composite bienzyme biosensors. , 2001, Analytical biochemistry.

[28]  Baoxin Li,et al.  Chemiluminescence flow biosensor for determination of total d-amino acid in serum with immobilized reagents , 2000 .

[29]  R. Upadhya,et al.  Stabilization of D-amino acid oxidase and catalase in permeabilized Rhodotorula gracilis cells and its application for the preparation of alpha-ketoacids*. , 2000, Biotechnology and bioengineering.

[30]  Rajan,et al.  Development of tailor-made glycidyl methacrylate-divinyl benzene copolymer for immobilization of D-amino acid oxidase from Aspergillus species strain 020 and its application in the bioconversion of cephalosporin C. , 1999, Journal of biotechnology.

[31]  Roberto Fernandez-Lafuente,et al.  The coimmobilization of d-amino acid oxidase and catalase enables the quantitative transformation of d-amino acids (d-phenylalanine) into α-keto acids (phenylpyruvic acid) , 1998 .

[32]  L. Pollegioni,et al.  A process for bioconversion of cephalosporin C by Rhodotorula gracilis D-amino acid oxidase , 1995, Biotechnology Letters.

[33]  K. Parkin,et al.  Immobilization and characterization of D–amino acid oxidase , 1979, Biotechnology and bioengineering.

[34]  Lu,et al.  Fullerene pipes , 1998, Science.