Catalytic Flow-Injection Determination of Sub-ppb Copper(II) Using the Redox Reaction of Cysteine with Iron(III) in the Presence of 2,4,6-Tris(2-pyridyl)-1,3,5-triazine

A kinetic-catalytic spectrophotometric flow-injection method was developed for the rapid and sensitive determination of trace amounts of copper(II). The method is based on the catalytic effect of copper(II) on the redox reaction of cysteine with iron(III). Iron(II) produced by the catalytic reaction reacts with 2,4,6-tris(2-pyridyl)-1,3,5-triazine (TPTZ) to form the iron(II)-TPTZ complex (lambda(max) = 593 nm). By measuring an absorbance of the complex, one could determine 0.05-8 ppb copper(II) with the relative standard deviations (n = 10) of 1.6%, 1.3%, and 0.8% for 0.5 ppb, 1 ppb, and 2 ppb copper(II), respectively. The limit of detection (S/N = 3) was 0.005 ppb. The sample throughput was 30 h(-1). The proposed method was successfully applied to the determination of copper in natural water and serum samples.

[1]  H. Katsumata,et al.  Flow-injection determination of copper(II) based on its catalysis on the redox reaction of cysteine with iron(III) in the presence of 1,10-phenanthroline. , 1999, Talanta.

[2]  N. Teshima,et al.  Kinetic method for the determination of iron(II, III) by its catalytic effect on the oxidation of 3-methyl-2-benzothiazolinone hydrazone with hydrogen peroxide , 1999 .

[3]  Y. Hagihara,et al.  Speciation of iron in river and tap waters by catalytic spectrophotometry using oxidation of o-phenylenediamine with hydrogen peroxide , 1999 .

[4]  Z. Zhi-qi,et al.  Catalytic simultaneous spectrophotometric determination of nitrite and nitrate with a flow injection system , 1998 .

[5]  H. Mao,et al.  Catalytic kinetic simultaneous determination of iron, silver and manganese with the Kalman filter by using flow injection analysis stopped-flow spectrophotometry. , 1998, Talanta.

[6]  K. Nakaso,et al.  Catalytic flow-injection determination of copper at nanogram levels by using color formation of N-phenyl-p-phenylenediamine with m-phenylenediamine in the presence of pyridine and ammonia as activators. , 1997, Talanta.

[7]  N. Teshima,et al.  Determination of ultratrace amounts of copper (II) by its catalytic effect on the oxidative coupling reaction of 3-methyl-2-benzothiazolinone hydrazone with N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline. , 1996, The Analyst.

[8]  M. Trojanowicz,et al.  Catalytic determination of copper in blood plasma using flow-injection biamperometry , 1993 .

[9]  M. Trojanowicz,et al.  Flow-injection ultraviolet spectrophotometric determination of sulphate in natural waters , 1990 .

[10]  Roger M. Smith,et al.  Spectrophotometric determination of copper as a dithiocarbamate by flow injection analysis , 1985 .

[11]  Fang Zhao-lun,et al.  The determination of trace amounts of heavy metals in waters by a flow-injection system including ion-exchange preconcentration and flame atomic absorption spectrometric detection , 1984 .

[12]  M. D. Castro,et al.  Catalytic-fluorimetric determination of copper at the nanograms per millilitre level by flow injection analysis , 1984 .

[13]  A. Hanaki,et al.  The Copper-catalyzed Autoxidation of Cysteine.The Amount of Hydrogen Peroxide Produced under Various Conditions and the Stoichiometry of the Reaction , 1983 .

[14]  R. Dessy,et al.  Potentiometric stripping with matrix exchange techniques in flow injection analysis of heavy metals in groundwaters , 1983 .

[15]  J. Janata,et al.  Combination of flow injection analysis and voltammetry , 1982 .

[16]  D. Koningsberger,et al.  The mechanism of the copper ion catalyzed autoxidation of cysteine in alkaline medium , 1981 .

[17]  N. Ishibashi,et al.  Flow injection—atomic absorption spectrometry with organic solvents , 1980 .

[18]  J. Caruso,et al.  Potentiometry and Potentiometric Titrations with the Hydrogen Electrode in Tetramethylene Sulfone , 1971 .