Characterization of new periodontal and endodontic isolates of spirochetes.

Gas chromatographic analysis of cellular fatty acids (CFA), biochemical reactions, electrophoresis of soluble cellular proteins, as well as immunodiffusion were used to discriminate between 16 fresh spirochete isolates from periodontal and endodontic infections. CFA patterns were compared by the Hewlett Packard MIDI library to all available reference strains, and results of the biochemical tests were compared to VPI's records for treponemal strains. The electrophoretograms of soluble proteins of the fresh isolates were compared to those of previously well described strains of Treponema denticola, T. pectinovorum, T. vincentii, T. socranskii subsp. socranskii, T. socranskii subsp. paredis, T. socranskii subsp. buccale, and T. socranskii 04. Immunodiffusion was carried out by using adsorbed polyclonal rabbit antibodies to representative strains of the species mentioned. These methods separated most clinical strains from approved species strains, suggesting that new species had been isolated.

[1]  I. Olsen,et al.  A new method for routine isolation of oral treponemes using U-tubes and pectin medium. , 1995, Anaerobe.

[2]  D. Caugant,et al.  Population genetic analysis of oral treponemes by multilocus enzyme electrophoresis. , 1995, Oral microbiology and immunology.

[3]  L. Moore,et al.  Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. , 1994, International journal of systematic bacteriology.

[4]  I. Olsen,et al.  Observation of an unusually large spirochete in endodontic infection. , 1993, Oral microbiology and immunology.

[5]  I. Olsen,et al.  Spirochaetes in oral infections. , 1993, Endodontics & dental traumatology.

[6]  L. Moore,et al.  Periodontal microflora of HIV positive subjects with gingivitis or adult periodontitis. , 1993, Journal of periodontology.

[7]  I. Olsen,et al.  Profiling of Propionibacterium acnes recovered from root canal and blood during and after endodontic treatment. , 1992, Endodontics & dental traumatology.

[8]  D. Adams,et al.  Identification of spirochetes related to Treponema pallidum in necrotizing ulcerative gingivitis and chronic periodontitis. , 1991, The New England journal of medicine.

[9]  L. Moore,et al.  Lactobacillus uli sp. nov. and Lactobacillus rimae sp. nov. from the human gingival crevice and emended descriptions of lactobacillus minutus and Streptococcus parvulus. , 1991, International journal of systematic bacteriology.

[10]  S. L. Barron,et al.  Use of monoclonal antibodies to enumerate spirochetes and identify Treponema denticola in dental plaque of children, adolescents and young adults. , 1991, Oral microbiology and immunology.

[11]  R. Fujinami,et al.  Is Theiler's murine encephalomyelitis virus infection of mice an autoimmune disease? , 1989, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[12]  W. Moore,et al.  Microbiology of periodontal disease. , 1987, Journal of periodontal research.

[13]  B. Tall,et al.  A clinical, demographic and microbiologic study of ANUG patients in an urban dental school. , 1987, Journal of clinical periodontology.

[14]  W. Moore,et al.  Bacteriology of Human Gingivitis , 1987, Journal of dental research.

[15]  B. Tall,et al.  Microscopic agglutination and polyacrylamide gel electrophoresis analyses of oral anaerobic spirochetes , 1986, Journal of clinical microbiology.

[16]  G. Greenstein,et al.  Microscopic monitoring of pathogens associated with periodontal diseases. A review. , 1985, Journal of periodontology.

[17]  R. M. Smibert,et al.  Treponema socranskii sp. nov., Treponema socranskii subsp. socranskii subsp. nov., Treponema socranskii subsp. buccale subsp. nov., and Treponema socranskii subsp. paredis subsp. nov. Isolated from the Human Periodontia , 1984 .

[18]  W. Moore,et al.  Bacteriology of experimental gingivitis in children , 1984, Infection and immunity.

[19]  C. Cobb,et al.  Acute necrotizing ulcerative gingivitis. A transmission electron microscope study. , 1983, Journal of periodontology.

[20]  J. Burmeister,et al.  Treponema pectinovorum sp. nov. isolated from humans with periodontitis , 1983 .

[21]  W E Moore,et al.  Bacteriology of severe periodontitis in young adult humans , 1982, Infection and immunity.

[22]  G. Armitage,et al.  Relationship between the percentage of subgingival spirochetes and the severity of periodontal disease. , 1982, Journal of periodontology.

[23]  W. Moore,et al.  Polyacrylamide Slab Gel Electrophoresis of Soluble Proteins for Studies of Bacterial Floras , 1980, Applied and environmental microbiology.