Immunologic and electrophoretic characteristics of two species of Crithidia.

Crithidia harmosa and Crithidia fasciculata were compared immunologically by the indirect fluorescent antibody (FA) method and by agglutination. The FA technic yielded more specific results with cellular-debris than with whole-cell antigens. The immune sera collected from chickens 9 days after the last inoculations with whole-cell antigens had higher homologous titers than those collected after 4 days. Major antigenic differences between the 2 species were revealed by both methods. The electrophoretic patterns of C. harmosa and C. fasciculata obtained by polyacrylamide gel slab electrophoresis differed in the numbers and relative mobilities of their component bands.

[1]  D. Dwyer.  Analysis of the antigenic relationships among Trichomonas, Histomonas, Dientamoeba, and Entamoeba. I. Quantitative fluorescent antibody methods. , 1972, The Journal of protozoology.

[2]  D. Dwyer.  Analysis of the antigenic relationships among Trichomonas, Histomonas, Dientamoeba, and Entamoeba. II. Gel diffusion methods. , 1972, The Journal of protozoology.

[3]  E. King.  Disc electrophoresis: avoiding artifacts caused by persulfate , 1970 .

[4]  R. McGhee,et al.  HOST SPECIFICITY OF VARIOUS SPECIES OF CRITHIDIA LEGER , 1970 .

[5]  J. Lenard.  Protein and glycolipid components of human erythrocyte membranes. , 1970, Biochemistry.

[6]  R. McGhee,et al.  Isolation, cloning and determination of biologic characteristics of five new species of Crithidia. , 1969, The Journal of protozoology.

[7]  J. Maizel,et al.  Molecular weight estimation of polypeptides by SDS-polyacrylamide gel electrophoresis: further data concerning resolving power and general considerations. , 1969, Analytical biochemistry.

[8]  M. Pothier,et al.  Valeur diagnostique des différentes formes parasitaires dans la trypanosomose américaine expérimentale en immuno-fluorescence chez la souris. , 1969 .

[9]  J V Maizel,et al.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. , 1967, Biochemical and biophysical research communications.

[10]  E. Vitetta,et al.  Immunological Relationships Among the Lower Trypanosomatidae , 1967 .

[11]  P. Akroyd.  Acrylamide gel slab electrophoresis in a simple glass cell for improved resolution and comparison of serum proteins. , 1967, Analytical biochemistry.

[12]  W. A. Siddiqui,et al.  Serological comparison of selected parasitic and free-living amoebae in vitro, using diffusion-precipitation and fluorescent-antibody technics. , 1966, The Journal of protozoology.

[13]  M. Tombs.  AN ELECTROPHORETIC INVESTIGATION OF GROUNDNUT PROTEINS: THE STRUCTURE OF ARACHINS A AND B. , 1965, The Biochemical journal.

[14]  B. Weitz.  THE SPECIFICITY OF TRYPANOSOMAL ANTIGENS BY IMMUNOFLUORESCENCE. , 1963, Journal of general microbiology.

[15]  J. Sargent,et al.  Properties of L-alpha-glycerophosphate oxidase and its role in the respiration of Trypanosoma rhodesiense. , 1960, The Biochemical journal.

[16]  R. McGhee.  Infection of chick embryos by Crithidia from a phytophagous heimpteron. , 1957, Science.

[17]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[18]  F. G. Wallace.  Flagellate Parasites of Mosquitoes with special Reference to Cvithidia fasciculata Léger, 1902. , 1943 .

[19]  H. Noguchi.  COMPARATIVE STUDIES OF HERPETOMONADS AND LEISHMANIAS II. DIFFERENTIATION OF THE ORGANISMS BY SEROLOGICAL REACTIONS AND FERMENTATION TESTS. , 1926 .

[20]  B. Latif,et al.  Differentiation of Trypanosoma brucei, T. rhodesiense, and T. gambiense by the indirect fluorescent antibody test. , 1973, Bulletin of the World Health Organization.

[21]  ANGELA E.R. Taylor.  Immunological studies on the subgenus Trypanozoon and Trypanosoma lewisi , 1968 .