Directed enzyme evolution.
暂无分享,去创建一个
F. Arnold | T. Bulter | E. Farinas | F H Arnold | E T Farinas | T Bulter
[1] A. Kosugi,et al. Synergistic Effects on Crystalline Cellulose Degradation between Cellulosomal Cellulases from Clostridium cellulovorans , 2002, Journal of bacteriology.
[2] A. Kosugi,et al. Thermostabilization of cellulosomal endoglucanase EngB from Clostridium cellulovorans by in vitro DNA recombination with non‐cellulosomal endoglucanase EngD , 2002, Molecular microbiology.
[3] J. Liao,et al. Alteration of Product Specificity of Rhodobacter sphaeroides Phytoene Desaturase by Directed Evolution* , 2001, The Journal of Biological Chemistry.
[4] H. Suenaga,et al. Directed Evolution of Biphenyl Dioxygenase: Emergence of Enhanced Degradation Capacity for Benzene, Toluene, and Alkylbenzenes , 2001, Journal of bacteriology.
[5] F. Arnold,et al. Patterns of adaptation in a laboratory evolved thermophilic enzyme. , 2001, Biochimica et biophysica acta.
[6] J. Seffernick,et al. Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes. , 2001, Chemistry & biology.
[7] W. Schwarz. The cellulosome and cellulose degradation by anaerobic bacteria , 2001, Applied Microbiology and Biotechnology.
[8] F. Arnold,et al. Laboratory Evolution of Toluene Dioxygenase To Accept 4-Picoline as a Substrate , 2001, Applied and Environmental Microbiology.
[9] F. Arnold,et al. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. , 2001, Protein engineering.
[10] F. Arnold,et al. Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae. , 2001, Biotechnology and bioengineering.
[11] Paul E. Swanson,et al. Rapid Evolution of Reversible Denaturation and Elevated Melting Temperature in a Microbial Haloalkane Dehalogenase , 2001 .
[12] F. Arnold,et al. Directed Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation , 2001 .
[13] W. Stemmer,et al. Directed evolution of proteins by exon shuffling , 2001, Nature Biotechnology.
[14] Volker Sieber,et al. Libraries of hybrid proteins from distantly related sequences , 2001, Nature Biotechnology.
[15] J. Reymond,et al. Novel methods for biocatalyst screening. , 2001, Current opinion in chemical biology.
[16] Philip T. Pienkos,et al. DNA shuffling method for generating highly recombined genes and evolved enzymes , 2001, Nature Biotechnology.
[17] D C Youvan,et al. Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase. , 2001, Protein engineering.
[18] Frances H. Arnold,et al. Computational method to reduce the search space for directed protein evolution , 2001, Proceedings of the National Academy of Sciences of the United States of America.
[19] Jennifer L. Ong,et al. Directed evolution of polymerase function by compartmentalized self-replication , 2001, Proceedings of the National Academy of Sciences of the United States of America.
[20] Jon E. Ness,et al. Predicting the emergence of antibiotic resistance by directed evolution and structural analysis , 2001, Nature Structural Biology.
[21] T. Suzuki,et al. Adaptation of a thermophilic enzyme, 3-isopropylmalate dehydrogenase, to low temperatures. , 2001, Protein engineering.
[22] F. Arnold,et al. How enzymes adapt: lessons from directed evolution , 2001, Trends in biochemical sciences.
[23] M. Reetz. Combinatorial and Evolution-Based Methods in the Creation of Enantioselective Catalysts. , 2001, Angewandte Chemie.
[24] A D Ellington,et al. In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates. , 2001, Journal of molecular biology.
[25] A. Kiener,et al. Industrial biocatalysis today and tomorrow , 2001, Nature.
[26] F. Arnold. Combinatorial and computational challenges for biocatalyst design , 2001, Nature.
[27] R. Sterner,et al. Thermophilic Adaptation of Proteins , 2001, Critical reviews in biochemistry and molecular biology.
[28] Frances H. Arnold,et al. Evolutionary Protein Design , 2001 .
[29] J. Lebbink,et al. beta-Glucosidase CelB from Pyrococcus furiosus: production by Escherichia coli, purification, and in vitro evolution. , 2001, Methods in enzymology.
[30] E. Toone,et al. Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli. , 2001, Structure.
[31] C. Mak,et al. Directed evolution of D-2-keto-3-deoxy-6-phosphogluconate aldolase to new variants for the efficient synthesis of D- and L-sugars. , 2000, Chemistry & biology.
[32] S. Ehrlich,et al. Efficient gene targeted random mutagenesis in genetically stable Escherichia coli strains. , 2000, Nucleic acids research.
[33] E. Cahoon,et al. Substrate-dependent mutant complementation to select fatty acid desaturase variants for metabolic engineering of plant seed oils. , 2000, Proceedings of the National Academy of Sciences of the United States of America.
[34] D. Pompon,et al. High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2. , 2000, Nucleic acids research.
[35] S. Karita,et al. A Large Gene Cluster for the Clostridium cellulovorans Cellulosome , 2000, Journal of bacteriology.
[36] Mark J. Olsen,et al. Function-based isolation of novel enzymes from a large library , 2000, Nature Biotechnology.
[37] T. Yoshimoto,et al. Directed evolution to improve the thermostability of prolyl endopeptidase. , 2000, Journal of biochemistry.
[38] M Wilmanns,et al. Directed evolution of a (beta alpha)8-barrel enzyme to catalyze related reactions in two different metabolic pathways. , 2000, Proceedings of the National Academy of Sciences of the United States of America.
[39] G. Winter,et al. Novel folded protein domains generated by combinatorial shuffling of polypeptide segments. , 2000, Proceedings of the National Academy of Sciences of the United States of America.
[40] G. Georgiou,et al. High-throughput screening of enzyme libraries. , 2000, Current opinion in biotechnology.
[41] Azevedo,et al. Effects of agitation level on the adsorption, desorption, and activities on cotton fabrics of full length and core domains of EGV (Humicola insolens) and CenA (Cellulomonas fimi). , 2000, Enzyme and microbial technology.
[42] Frances H. Arnold,et al. Molecular breeding of carotenoid biosynthetic pathways , 2000, Nature Biotechnology.
[43] J. Sutherland,et al. Evolutionary optimisation of enzymes. , 2000, Current opinion in chemical biology.
[44] C. Schmidt-Dannert,et al. Engineering novel carotenoids in microorganisms. , 2000, Current opinion in biotechnology.
[45] A. Szilágyi,et al. Structural differences between mesophilic, moderately thermophilic and extremely thermophilic protein subunits: results of a comprehensive survey. , 2000, Structure.
[46] F. Arnold,et al. Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris. , 2000, Protein engineering.
[47] F. Arnold,et al. Directed evolution study of temperature adaptation in a psychrophilic enzyme. , 2000, Journal of molecular biology.
[48] J. Mccoy,et al. [21] Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli , 2000 .
[49] M. Oh,et al. Directed Evolution of Metabolically Engineered Escherichiacoli for Carotenoid Production , 2000, Biotechnology progress.
[50] F. Arnold,et al. Temperature adaptation of enzymes: lessons from laboratory evolution. , 2000, Advances in protein chemistry.
[51] S. Benkovic,et al. Evolution of protein function by domain swapping. , 2000, Advances in protein chemistry.
[52] M. Wilmanns,et al. Directed evolution of a ( ba ) 8-barrel enzyme to catalyze related reactions in two different metabolic pathways , 2000 .
[53] Marc Ostermeier,et al. A combinatorial approach to hybrid enzymes independent of DNA homology , 1999, Nature Biotechnology.
[54] Frances H. Arnold,et al. Exploring Nonnatural Evolutionary Pathways by Saturation Mutagenesis: Rapid Improvement of Protein Function , 1999, Journal of Molecular Evolution.
[55] J. McDonald,et al. Patterns of temperature adaptation in proteins from Methanococcus and Bacillus. , 1999, Molecular biology and evolution.
[56] M. Oh,et al. Engineered isoprenoid pathway enhances astaxanthin production in Escherichia coli. , 1999, Biotechnology and bioengineering.
[57] M. Zaccolo,et al. The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase. , 1999, Journal of molecular biology.
[58] F. Arnold,et al. Functional Expression of Horseradish Peroxidase in E.coli by Directed Evolution , 1999, Biotechnology progress.
[59] Dan S. Tawfik,et al. Man-made cell-like compartments for molecular evolution , 1998, Nature Biotechnology.
[60] R. Doi,et al. Cellulosome and noncellulosomal cellulases of Clostridium cellulovorans , 1998, Extremophiles.
[61] Frances H. Arnold,et al. Molecular evolution by staggered extension process (StEP) in vitro recombination , 1998, Nature Biotechnology.
[62] S. Ito. Alkaline cellulases from alkaliphilic Bacillus: Enzymatic properties, genetics, and application to detergents , 1997, Extremophiles.
[63] B K Shoichet,et al. A relationship between protein stability and protein function. , 1995, Proceedings of the National Academy of Sciences of the United States of America.
[64] W. Stemmer. DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. , 1994, Proceedings of the National Academy of Sciences of the United States of America.
[65] W. Stemmer. Rapid evolution of a protein in vitro by DNA shuffling , 1994, Nature.
[66] Kurt Faber,et al. Biotransformations in Organic Chemistry , 1992 .
[67] L. Lynd,et al. Fuel Ethanol from Cellulosic Biomass , 1991, Science.
[68] O. Shoseyov,et al. Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase. , 1990, Proceedings of the National Academy of Sciences of the United States of America.
[69] P. Wood,et al. Use of complex formation between Congo Red and polysaccharides in detection and assay of polysaccharide hydrolases , 1988 .
[70] R. Jensen. Enzyme recruitment in evolution of new function. , 1976, Annual review of microbiology.