论文信息 - Simultaneous 3D imaging of morphology and nanoparticle distribution in single cells with the Cell-CT™ technology

Simultaneous 3D imaging of morphology and nanoparticle distribution in single cells with the Cell-CT™ technology

The Cell-CT™ is an optical projection tomography microscope (OPTM) developed for high resolution 3D imaging of single cells based on absorption stains and brightfield microscopy. In this study we demonstrate the use of the Cell-CT™ in multi-color mode for simultaneous imaging of cellular 3D morphology and the 3D distribution of nanoparticle clusters in the cytoplasm. The ability to image cellular processes in relation to cellular compartments with a non-fluorescence 3D technology opens new perspectives for molecular research.

[1] Anna Moore,et al. In vivo imaging of siRNA delivery and silencing in tumors , 2007, Nature Medicine.

[2] C Cremer,et al. Axial tomographic confocal fluorescence microscopy , 2002, Journal of microscopy.

[3] Ralph Weissleder,et al. Differential conjugation of tat peptide to superparamagnetic nanoparticles and its effect on cellular uptake. , 2002, Bioconjugate chemistry.

[4] Xiaoping P. Hu,et al. Functionalization and peptide-based delivery of magnetic nanoparticles as an intracellular MRI contrast agent , 2004, JBIC Journal of Biological Inorganic Chemistry.

[5] Agard,et al. I5M: 3D widefield light microscopy with better than 100 nm axial resolution , 1999, Journal of microscopy.

[6] Thomas Neumann,et al. Three-dimensional imaging of single isolated cell nuclei using optical projection tomography. , 2005, Optics express.

[7] S. Hell,et al. 4Pi‐confocal images with axial superresolution , 1996 .

[9] M. Gustafsson,et al. Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy , 2008, Science.

[10] Eric J. Seibel,et al. Automated cell analysis in 2D and 3D: A comparative study , 2009, Pattern Recognit..

[11] C. Sheppard,et al. Theory and practice of scanning optical microscopy , 1984 .