13C and 1H NMR Study of Lysophosphatidylcholine (LPC) Isolated from the Surface of Wheat Starch Granules

Lysophosphatidylcholine (LPC) has been isolated from the surface of starch granules by extraction and subsequent chromatographic purification. The purified extract was examined by mono and bidimensional 1H and 13C NMR and identified as LPC. The NMR information also allowed the assignment of the length and nature of the acyl chains (18—20 carbon atoms, saturated). Se ha aislado lisofosfatidilcolina (LPC) de la superficie de gránulos de almidón mediante extracción y posterior purificación cromatográfica. El extracto purificado se analizó por espectroscopía de RMN de 1H y 13C mono y bidimensional, lo que permitió identificarlo como LPC. Las cadenas acilo se identificaron como cadenas pertenecientes a ácidos saturados de 18—20 átomos de carbono.

[1]  O. K. Chung,et al.  Volatile compounds in five starches , 2000 .

[2]  Zhimin Zhou,et al.  Multi‐dimensional NMR structural analyses of purified Lipid X and Lipid A (endotoxin) , 1999 .

[3]  Z. Czuchajowska,et al.  Role of Phosphorus in Viscosity, Gelatinization, and Retrogradation of Starch , 1998 .

[4]  M. Seguchi,et al.  Structure of Lysophosphatidylglyceryl-Remazolbrilliant Blue (LPG-RBB) from the Surface of Dyed Wheat Starch Granules , 1998 .

[5]  A. Eliasson,et al.  The effect of sodium dodecylsulfate on gelatinization and gelation properties of wheat and potato starches , 1998 .

[6]  P. Reinikainen,et al.  Starch Lipids of Barley and Malt , 1997 .

[7]  M. Seguchi,et al.  Study of three-dimensional structure of wheat starch granules stained with remazolbrilliant blue dye and extracted with aqueous sodium dodecyl sulfate and mercaptoethanol solution , 1997 .

[8]  P. Colonna,et al.  Limiting factors of starch hydrolysis. , 1992, European journal of clinical nutrition.

[9]  S. Paoletti,et al.  1H-NMR studies of lipid extracts of rat liver mitochondria. , 1991, Biochemical and biophysical research communications.

[10]  R. Zidovetzki,et al.  Analysis of membrane lipids by 500 MHz 1H NMR. , 1989, Analytical biochemistry.

[11]  K. Kitahara,et al.  Release of Bound Lipids in Cereal Starches Upon Hydrolysis by Glucoamylase , 1997 .

[12]  M. Seguchi Surface staining of wheat starch granules with remazolbrilliant blue R dye and their extraction with aqueous sodium dodecyl sulfate and mercaptoethanol , 1995 .

[13]  A. Hermansson,et al.  Distribution of Amylose and Amylopectin in Potato Starch Pastes: Effects of Heating and Shearing , 1991 .

[14]  T. Kwon,et al.  Morphology and Composition , 1987 .

[15]  K. A. Gilles,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[16]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .