USING OF ELSD FOR DETERMINATION OF ORGANIC ENGINEERED NANOPARTICLES

In presented study determination of various organic engineered nanoparticles ENPs, with cross-linked gelatine and/or polysorbate based nanoparticles in water and beverages was examined. For this purpose Ultra Performance Liquid Chromatography (UPLC) Size Exclusion Chromatography (SEC) coupled with Evaporative Light Scattering Detector (ELSD) was used. Analytical method for characterisation and determination of nanoparticles in food matrices was developed; sample preparation procedure and parameters of determination of ENPs were optimised to achieve the best performance characteristics. For cross-linked gelatine various storage and hydrolysis conditions were tested to verify the stability of prepared cross-linked gelatine. The limit of quantification (LOQ) of organic ENPs was: (i) for cross-linked gelatine 1 mg/mL in beverages and 0.5 mg/mL in water and (ii) for polysorbate based ENPs 0.5 mg/mL in beverages and 0.1 mg/mL in water.

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