Recent advances in the microbial safety of fresh fruits and vegetables.

Foodborne illness outbreaks linked to fresh produce are becoming more frequent and widespread. High impact outbreaks, such as that associated with spinach contaminated with Escherichia coli O157:H7, resulted in almost 200 cases of foodborne illness across North America and >$300 m market losses. Over the last decade there has been intensive research into gaining an understanding on the interactions of human pathogens with plants and how microbiological safety of fresh produce can be improved. The following review will provide an update on the food safety issues linked to fresh produce. An overview of recent foodborne illness outbreaks linked to fresh produce. The types of human pathogens encountered will be described and how they can be transferred from their normal animal or human host to fresh produce. The interaction of human pathogens with growing plants will be discussed, in addition to novel intervention methods to enhance the microbiological safety of fresh produce.

[1]  L. Cheng,et al.  Soil temperature and plant growth stage influence nitrogen uptake and amino acid concentration of apple during early spring growth. , 2001, Tree physiology.

[2]  R. P. Pund,et al.  Die Bedeutung der beweglichen Aeromonaden als Krankheitserreger für den Menschen , 2008, Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz.

[3]  Anderson Bc Effect of drying on the infectivity of cryptosporidia-laden calf feces for 3- to 7-day-old mice. , 1986 .

[4]  Joseph Odumeru,et al.  Microbial survey of selected Ontario-grown fresh fruits and vegetables. , 2007, Journal of food protection.

[5]  R. Brackett,et al.  Survival of Salmonella on tomatoes stored at high relative humidity, in soil, and on tomatoes in contact with soil. , 2002, Journal of food protection.

[6]  A. Hartmann,et al.  Colonization of barley (Hordeum vulgare) with Salmonella enterica and Listeria spp. , 2006, FEMS microbiology ecology.

[7]  Shinichi Kawamoto,et al.  Efficacy of acidified sodium chlorite treatments in reducing Escherichia coli O157:H7 on Chinese cabbage. , 2005, Journal of food protection.

[8]  R. Saftner,et al.  Improving biocontrol using antagonist mixtures with heat and/or sodium bicarbonate to control postharvest decay of apple fruit , 2005 .

[9]  S. Phatak,et al.  Persistence of enterohemorrhagic Escherichia coli O157:H7 in soil and on leaf lettuce and parsley grown in fields treated with contaminated manure composts or irrigation water. , 2004, Journal of food protection.

[10]  S. Goyal,et al.  Effect of temperature and sanitizers on the survival of feline calicivirus, Escherichia coli, and F-specific coliphage MS2 on leafy salad vegetables. , 2004, Journal of food protection.

[11]  H. Ramaswamy,et al.  Effect of high pressure processing on the texture of selected fruits and vegetables , 1998 .

[12]  M. Loessner,et al.  Virulent Bacteriophage for Efficient Biocontrol of Listeria monocytogenes in Ready-To-Eat Foods , 2008, Applied and Environmental Microbiology.

[13]  Dr. Dr. h.c. Eilhard Mitscherlich,et al.  Microbial Survival in the Environment , 1984, Springer Berlin Heidelberg.

[14]  A. Klančnik,et al.  Environmental stress factors affecting survival and virulence of Campylobacter jejuni. , 2007, Microbial pathogenesis.

[15]  E. Groisman,et al.  Phenotypic differences between Salmonella and Escherichia coli resulting from the disparate regulation of homologous genes. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[16]  M. Fuchigami,et al.  Frozen Carrots Texture and Pectic Compotients as Affected by Low‐Temperature‐Blanching and Quick Freezing , 1995 .

[17]  K. Warriner,et al.  Spatial distribution of Salmonella, Escherichia coli O157:H7, and other bacterial populations in commercial and laboratory-scale sprouting mung bean beds. , 2005, Journal of food protection.

[18]  A. L. Iniguez,et al.  Regulation of enteric endophytic bacterial colonization by plant defenses. , 2005, Molecular plant-microbe interactions : MPMI.

[19]  M. Brandl,et al.  Leaf Age as a Risk Factor in Contamination of Lettuce with Escherichia coli O157:H7 and Salmonella enterica , 2008, Applied and Environmental Microbiology.

[20]  E S Krampitz,et al.  Longevity of pathogenic bacteria especially Salmonella in cistern water. , 1999, Zentralblatt fur Hygiene und Umweltmedizin = International journal of hygiene and environmental medicine.

[21]  G. Duffy Verocytoxigenic Escherichia coli in animal faeces, manures and slurries , 2003, Journal of applied microbiology.

[22]  M. Vassileva,et al.  Simultaneous P-solubilizing and biocontrol activity of microorganisms: potentials and future trends , 2006, Applied Microbiology and Biotechnology.

[23]  A. Cassells,et al.  Investigations into the persistence of Escherichia coli endophytically, in cabbage (Brassica oleracea var. capitata L.) and associated alteration in host proteins and chitinase activity , 2003 .

[24]  Daniel A. Okun,et al.  Guidelines for water reuse. , 1992 .

[25]  F. Gozzo Systemic acquired resistance in crop protection: from nature to a chemical approach. , 2003, Journal of agricultural and food chemistry.

[26]  L. Lang Investigation of outbreak of infections caused by Salmonella Saintpaul. , 2008, Gastroenterology.

[27]  J F Frank,et al.  Penetration of Escherichia coli O157:H7 into lettuce tissues as affected by inoculum size and temperature and the effect of chlorine treatment on cell viability. , 2000, Journal of food protection.

[28]  Chantal Smout,et al.  Effects of high-pressure pretreatment and calcium soaking on the texture degradation kinetics of carrots during thermal processing , 2006 .

[29]  N. Bean,et al.  The changing epidemiology of salmonella: trends in serotypes isolated from humans in the United States, 1987-1997. , 2001, The Journal of infectious diseases.

[30]  D. Gordon Geographical structure and host specificity in bacteria and the implications for tracing the source of coliform contamination. , 2001, Microbiology.

[31]  G. Bending,et al.  Human pathogens and the phyllosphere. , 2008, Advances in applied microbiology.

[32]  R. Saftner,et al.  Fresh-cut lettuce in modified atmosphere packages stored at improper temperatures supports enterohemorrhagic E. coli isolates to survive gastric acid challenge. , 2008, Journal of food science.

[33]  K. Kniel,et al.  High hydrostatic pressure and UV light treatment of produce contaminated with Eimeria acervulina as a Cyclospora cayetanensis surrogate. , 2007, Journal of food protection.

[34]  M. Wiedmann,et al.  Microarray-Based Characterization of the Listeria monocytogenes Cold Regulon in Log- and Stationary-Phase Cells , 2007, Applied and Environmental Microbiology.

[35]  Yaguang Luo,et al.  Efficacy of sanitizers to inactivate Escherichia coli O157:H7 on fresh-cut carrot shreds under simulated process water conditions. , 2004, Journal of food protection.

[36]  M. Dickinson,et al.  Interaction of Escherichia coli with growing salad spinach plants. , 2003, Journal of food protection.

[37]  J. C. Hoff,et al.  Effect of chlorine treatment on infectivity of hepatitis A virus , 1983, Applied and environmental microbiology.

[38]  Thor Axel Stenström,et al.  Faecal contamination of greywater and associated microbial risks. , 2003, Water research.

[39]  Elliot T Ryser,et al.  A comparison of different chemical sanitizers for inactivating Escherichia coli O157:H7 and Listeria monocytogenes in solution and on apples, lettuce, strawberries, and cantaloupe. , 2004, Journal of food protection.

[40]  R. Mandrell,et al.  Prevalence of Escherichia coli associated with a cabbage crop inadvertently irrigated with partially treated sewage wastewater. , 2002, Journal of food protection.

[41]  M. Woolhouse,et al.  Super-shedding and the link between human infection and livestock carriage of Escherichia coli O157 , 2008, Nature Reviews Microbiology.

[42]  R. Fayer,et al.  Cryptosporidium and Cryptosporidiosis , 1997 .

[43]  Britta Leverentz,et al.  Optimizing concentration and timing of a phage spray application to reduce Listeria monocytogenes on honeydew melon tissue. , 2004, Journal of food protection.

[44]  S. Lindow,et al.  Microbiology of the Phyllosphere , 2003, Applied and Environmental Microbiology.

[45]  C. Ray,et al.  An evaluation of the mobility of pathogen indicators, Escherichia coli and bacteriophage MS-2, in a highly weathered tropical soil under unsaturated conditions. , 2008, Journal of water and health.

[46]  K. Warriner,et al.  Persistence and growth of different Salmonella serovars on pre- and postharvest tomatoes. , 2007, Journal of food protection.

[47]  A. A. Mahboubi,et al.  Influence of organic waste type and soil structure on the bacterial filtration rates in unsaturated intact soil columns. , 2009, Journal of environmental management.

[48]  O. Holst,et al.  Host and Non‐Host Plant Response to Bacterial Wilt in Potato: Role of the Lipopolysaccharide Isolated from Ralstonia solanacearum and Molecular Analysis of Plant–Pathogen Interaction , 2008, Chemistry & biodiversity.

[49]  M. Wiedmann,et al.  σB-Dependent and σB-Independent Mechanisms Contribute to Transcription of Listeria monocytogenes Cold Stress Genes during Cold Shock and Cold Growth , 2007, Applied and Environmental Microbiology.

[50]  J. Marcy,et al.  Inhibition of pathogens on fresh produce by ultraviolet energy. , 2004, International journal of food microbiology.

[51]  M. Brandl Plant Lesions Promote the Rapid Multiplication of Escherichia coli O157:H7 on Postharvest Lettuce , 2008, Applied and Environmental Microbiology.

[52]  T A Hill,et al.  Recurrent multistate outbreak of Salmonella Newport associated with tomatoes from contaminated fields, 2005 , 2007, Epidemiology and Infection.

[53]  I. Seymour,et al.  Foodborne viruses and fresh produce , 2001, Journal of applied microbiology.

[54]  S. Monday,et al.  Microbiological Quality of Bagged Cut Spinach and Lettuce Mixes , 2007, Applied and Environmental Microbiology.

[55]  S. Loncarevic,et al.  Bacteriological quality of organically grown leaf lettuce in Norway , 2005, Letters in applied microbiology.

[56]  G. Oron,et al.  Surface and subsurface irrigation with effluents of different qualities and presence of Cryptosporidium oocysts in soil and on crops. , 2002, Water science and technology : a journal of the International Association on Water Pollution Research.

[57]  M. Brown,et al.  Sample Size, Library Composition, and Genotypic Diversity among Natural Populations of Escherichia coli from Different Animals Influence Accuracy of Determining Sources of Fecal Pollution , 2004, Applied and Environmental Microbiology.

[58]  J. Karns,et al.  Leaching of Escherichia coli O157:H7 in Diverse Soils under Various Agricultural Management Practices , 2000, Applied and Environmental Microbiology.

[59]  Eduardo A. Groisman,et al.  Role of Nonhost Environments in the Lifestyles of Salmonella and Escherichia coli , 2003, Applied and Environmental Microbiology.

[60]  J. Sidhu,et al.  Human pathogens and their indicators in biosolids: a literature review. , 2009, Environment international.

[61]  B. Barbeau,et al.  Protection of waterborne pathogens by higher organisms in drinking water: a review. , 2008, Canadian journal of microbiology.

[62]  K. Wan,et al.  Risk factors for endemic giardiasis: highlighting the possible association of contaminated water and food. , 2008, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[63]  L. Robertson,et al.  Survival of Cryptosporidium parvum oocysts under various environmental pressures , 1992, Applied and environmental microbiology.

[64]  C. Billington,et al.  Bacteriophages as biocontrol agents in food. , 2005, Journal of food protection.

[65]  K. Matthews Microbiology of fresh produce. , 2006 .

[66]  J. Guentzel,et al.  Reduction of bacteria on spinach, lettuce, and surfaces in food service areas using neutral electrolyzed oxidizing water. , 2008, Food microbiology.

[67]  Emma L Snary,et al.  Antimicrobial resistance: a microbial risk assessment perspective. , 2004, The Journal of antimicrobial chemotherapy.

[68]  J. Lalucat,et al.  Comparative reductions of bacterial indicators, bacteriophage-infecting enteric bacteria and enteroviruses in wastewater tertiary treatments by lagooning and UV-radiation. , 2008, Water science and technology : a journal of the International Association on Water Pollution Research.

[69]  Charles P Gerba,et al.  The persistence and removal of enteric pathogens in constructed wetlands. , 2004, Water research.

[70]  R. Nayak,et al.  Molecular epidemiological analysis and microbial source tracking of Salmonella enterica serovars in a preharvest turkey production environment. , 2008, Foodborne pathogens and disease.

[71]  G. Mittal,et al.  Inactivation of MS2 F(+) coliphage on lettuce by a combination of UV light and hydrogen peroxide. , 2008, Journal of food protection.

[72]  J. Ryu,et al.  Produce handling and processing practices. , 1997, Emerging infectious diseases.

[73]  J. Lejeune,et al.  Coliforms and prevalence of Escherichia coli and foodborne pathogens on minimally processed spinach in two packing plants. , 2008, Journal of food protection.

[74]  K. Jones,et al.  Microbial contamination of fruit and vegetables and the behaviour of enteropathogens in the phyllosphere: a review , 2008, Journal of applied microbiology.

[75]  Yun-Hee Kim,et al.  Occurrence of Cryptosporidium oocysts and Giardia cysts in the Nakdong River and their removal during water treatment. , 2007, Journal of Water and Health.

[76]  Eelco Franz,et al.  Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiency , 2007, The ISME Journal.

[77]  J. Jofre,et al.  Bacterial host strains that support replication of somatic coliphages , 2004, Antonie van Leeuwenhoek.

[78]  R. Sikora,et al.  Detection of fungi in planta: effectiveness of surface sterilization methods , 2001 .

[79]  M. Hutchison,et al.  Levels of zoonotic agents in British livestock manures , 2004, Letters in applied microbiology.

[80]  M. Bronze,et al.  Listeria monocytogenes: epidemiology, human disease, and mechanisms of brain invasion. , 2008, FEMS immunology and medical microbiology.

[81]  D. Smith,et al.  Animal growth promoters: to ban or not to ban? A risk assessment approach. , 2004, International journal of antimicrobial agents.

[82]  B. Chambers,et al.  Pathogen survival during livestock manure storage and following land application. , 2005, Bioresource technology.

[83]  L. Chacin-Bonilla Transmission of Cyclospora cayetanensis infection: a review focusing on soil-borne cyclosporiasis. , 2008, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[84]  T. Ochoa,et al.  New insights into the epidemiology of enteropathogenic Escherichia coli infection. , 2008, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[85]  U. Kidmose,et al.  Changes in texture, microstructure and nutritional quality of carrot slices during blanching and freezing , 1999 .

[86]  Euan K. James,et al.  Infection and Colonization of Sugar Cane and Other Graminaceous Plants by Endophytic Diazotrophs , 1998 .

[87]  K. Mølbak,et al.  A large, multiple-restaurant outbreak of infection with Shigella flexneri serotype 2a traced to tomatoes. , 2006, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[88]  J F Frank,et al.  Comparison of the attachment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, and Pseudomonas fluorescens to lettuce leaves. , 2000, Journal of food protection.

[89]  N. Bernstein,et al.  Assessment of contamination potential of lettuce by Salmonella enterica serovar Newport added to the plant growing medium. , 2007, Journal of food protection.

[90]  R. Tillett,et al.  Inferring pathogen inactivation from the surface temperatures of compost heaps. , 2005, Bioresource technology.

[91]  M. Dickinson,et al.  Internalization of bioluminescent Escherichia coli and Salmonella Montevideo in growing bean sprouts , 2003, Journal of applied microbiology.

[92]  G. Johnsen,et al.  Outbreak of Shigella sonnei infection traced to imported iceberg lettuce , 1995, Journal of clinical microbiology.

[93]  K. Tang,et al.  Bioactive natural products from endophytes: A review , 2008, Applied Biochemistry and Microbiology.

[94]  S. Sattar,et al.  Survival of calicivirus in foods and on surfaces: experiments with feline calicivirus as a surrogate for norovirus. , 2007, Journal of food protection.

[95]  Embil Ja,et al.  Surveillance of Listeria monocytogenes in human and environmental specimens in Nova Scotia, 1974 to 1981. , 1984 .

[96]  M. Dardé,et al.  How to detect Toxoplasma gondii oocysts in environmental samples? , 2003, FEMS microbiology reviews.

[97]  V. Lund,et al.  Detection of Small Numbers of Campylobacter jejuni and Campylobacter coli Cells in Environmental Water, Sewage, and Food Samples by a Seminested PCR Assay , 1999, Applied and Environmental Microbiology.

[98]  J. Frank,et al.  Quantitative determination of the role of lettuce leaf structures in protecting Escherichia coli O157:H7 from chlorine disinfection. , 2001, Journal of food protection.

[99]  A. V. Van Bruggen,et al.  Influence of temperature fluctuations on Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in cow manure. , 2007, FEMS microbiology ecology.

[100]  F. Ausubel,et al.  Pathogenesis of the human opportunistic pathogen Pseudomonas aeruginosa PA14 in Arabidopsis. , 2000, Plant physiology.

[101]  T. Dejong,et al.  Interaction Between Nitrogen-Fertilized Peach Trees and Expression of syrB, a Gene Involved in Syringomycin Production in Pseudomonas syringae pv. syringae. , 2005, Phytopathology.

[102]  Antonio Delgado,et al.  Numerical simulation of the mechanics of a yeast cell under high hydrostatic pressure. , 2004, Journal of biomechanics.

[103]  R. Tan,et al.  Endophytes: a rich source of functional metabolites. , 2001, Natural product reports.

[104]  M. Foran,et al.  The effect of farm liquid waste application on tile drainage , 1992 .

[105]  R. Mandrell,et al.  Comparison of Survival of Campylobacter jejuni in the Phyllosphere with That in the Rhizosphere of Spinach and Radish Plants , 2004, Applied and Environmental Microbiology.

[106]  Alexandra Lianou,et al.  A review of the incidence and transmission of Listeria monocytogenes in ready-to-eat products in retail and food service environments. , 2007, Journal of food protection.

[107]  M. Nakauma,et al.  Effectiveness of irradiation treatments in inactivating Listeria monocytogenes on fresh vegetables at refrigeration temperature. , 2005, Journal of food protection.

[108]  W. Chao,et al.  Survival of Yersinia enterocolitica in the environment. , 1988, Canadian journal of microbiology.

[109]  P. Tchounwou,et al.  Impact of Animal Waste Application on Runoff Water Quality in Field Experimental Plots , 2005, International journal of environmental research and public health.

[110]  Z. Dou,et al.  Survival of Salmonella enterica Serovar Newport in Manure and Manure-Amended Soils , 2006, Applied and Environmental Microbiology.

[111]  C. Keys,et al.  Incidence and Tracking of Escherichia coli O157:H7 in a Major Produce Production Region in California , 2007, PloS one.

[112]  R. Greco Preferential flow in macroporous swelling soil with internal catchment: model development and applications , 2002 .

[113]  Oliver Thimm,et al.  Insights into metabolism obtained from microarray analysis. , 2003, Current opinion in plant biology.

[114]  J. Wells,et al.  Fate of Shigella sonnei on parsley and methods of disinfection. , 2000, Journal of food protection.

[115]  Vicente Navarro,et al.  Bacteriemia por Aeromonas spp.: estudio de 12 episodios y revisión de la literatura , 2001 .

[116]  J. R. Gorny,et al.  Microbiology Of Fruits And Vegetables , 2020 .

[117]  V. Baldani,et al.  Recent advances in BNF with non-legume plants , 1997 .

[118]  A. Farnleitner,et al.  Quantitative microbial faecal source tracking with sampling guided by hydrological catchment dynamics , 2008, Environmental microbiology.

[119]  Paul J. Cieslak,et al.  Escherichia coli 0157:H7 infection from a manured garden , 1993, The Lancet.

[120]  S. Stringer,et al.  The microbiological quality of hot water‐washed broccoli florets and cut green beans , 2007, Journal of applied microbiology.

[121]  Britt Erickson IRRADIATION OF LETTUCE & SPINACH: Critics fear rule will REDUCE NUTRITIONAL VALUE of food, hinder efforts to improve unsanitary practices , 2008 .

[122]  M. Karapınar,et al.  Survival of Yersinia enterocolitica and Escherichia coli in spring water. , 1991, International journal of food microbiology.

[123]  M. Peck,et al.  Growth of and toxin production by nonproteolytic Clostridium botulinum in cooked puréed vegetables at refrigeration temperatures , 1996, Applied and environmental microbiology.

[124]  W. L. Araújo,et al.  Diversity of endophytic enterobacteria associated with different host plants , 2008, The Journal of Microbiology.

[125]  T. Stenström,et al.  Identification and management of microbial contaminations in a surface drinking water source. , 2007, Journal of water and health.

[126]  G. M. Sapers,et al.  Efficacy of 1% Hydrogen Peroxide Wash in Decontaminating Apples and Cantaloupe Melons , 2003 .

[127]  J. Garland,et al.  Effects of community versus single strain inoculants on the biocontrol of Salmonella and microbial community dynamics in alfalfa sprouts. , 2005, Journal of food protection.

[128]  P. Butz,et al.  Changes in functional properties of vegetables induced by high pressure treatment , 2002 .

[129]  M. Rohde,et al.  The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix , 2001, Molecular microbiology.

[130]  H. Suty,et al.  Applications of advanced oxidation processes: present and future. , 2004, Water science and technology : a journal of the International Association on Water Pollution Research.

[131]  L. Beuchat,et al.  Fate of Salmonella montevideo on and in raw tomatoes as affected by temperature and treatment with chlorine , 1995, Applied and environmental microbiology.

[132]  P. Delaquis,et al.  Effect of wash water temperature and chlorination on phenolic metabolism and browning of stored iceberg lettuce photosynthetic and vascular tissues. , 2002, Journal of agricultural and food chemistry.

[133]  A. Castillo,et al.  Internalization of bacterial pathogens in tomatoes and their control by selected chemicals. , 2004, Journal of food protection.

[134]  L. Lang FDA approves use of bacteriophages to be added to meat and poultry products. , 2006, Gastroenterology.

[135]  G. Arroyo,et al.  High Hydrostatic Pressure Effects on Vegetable Structure , 1998 .

[136]  J. Sheridan,et al.  Washing and chilling as critical control points in pork slaughter hazard analysis and critical control point (HACCP) systems , 2002, Journal of applied microbiology.

[137]  D. Cliver,et al.  Survival of Escherichia coli O157:H7 and Salmonella typhimurium in cow manure and cow manure slurry. , 1999, FEMS microbiology letters.

[138]  A. Ibekwe,et al.  Fate of Escherichia coli O157:H7 in irrigation water on soils and plants as validated by culture method and real-time PCR. , 2004, Canadian journal of microbiology.

[139]  E. Solomon,et al.  Transmission of Escherichia coli O157:H7 from Contaminated Manure and Irrigation Water to Lettuce Plant Tissue and Its Subsequent Internalization , 2002, Applied and Environmental Microbiology.

[140]  E. Martínez-Romero,et al.  Bacterial endophytes and their interactions with hosts. , 2006, Molecular plant-microbe interactions : MPMI.

[141]  W.M.F. Jongen,et al.  Improving the Safety of Fresh Fruit and Vegetables , 2005 .

[142]  M. Menetrez,et al.  Bacteriophages Reduce Experimental Contamination of Hard Surfaces, Tomato, Spinach, Broccoli, and Ground Beef by Escherichia coli O157:H7 , 2008, Applied and Environmental Microbiology.

[143]  V. Dato,et al.  Green onions: potential mechanism for hepatitis A contamination. , 2006, Journal of food protection.

[144]  Srinand Sreevatsan,et al.  Persistence of Mycobacterium avium subsp. paratuberculosis and Other Zoonotic Pathogens during Simulated Composting, Manure Packing, and Liquid Storage of Dairy Manure , 2006, Applied and Environmental Microbiology.

[145]  B. Lund Ecosystems in vegetable foods. , 1992, Society for Applied Bacteriology symposium series.

[146]  E. Triplett,et al.  Plants as a habitat for beneficial and/or human pathogenic bacteria. , 2008, Annual review of phytopathology.

[147]  S. Isobe,et al.  Effect of ozonated water treatment on microbial control and on browning of iceberg lettuce (Lactuca sativa L.). , 2006, Journal of food protection.

[148]  Yaguang Luo,et al.  Fresh-cut Produce Wash Water Reuse Affects Water Quality and Packaged Product Quality and Microbial Growth in Romaine Lettuce , 2007 .

[149]  K. Giller,et al.  Long-term effects of metals in sewage sludge on soils, microorganisms and plants , 1995, Journal of Industrial Microbiology.

[150]  Stefan Wuertz,et al.  Quo vadis source tracking? Towards a strategic framework for environmental monitoring of fecal pollution. , 2007, Water research.

[151]  R. Hendrix,et al.  Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[152]  M. Brandl,et al.  Fitness of human enteric pathogens on plants and implications for food safety. , 2006, Annual review of phytopathology.

[153]  E. Solomon,et al.  Interaction of live and dead Escherichia coli O157:H7 and fluorescent microspheres with lettuce tissue suggests bacterial processes do not mediate adherence , 2006, Letters in applied microbiology.

[154]  M. Kieny,et al.  A review of vaccine research and development: human enteric infections. , 2006, Vaccine.

[155]  Lee-Ann Jaykus,et al.  A field study of the microbiological quality of fresh produce. , 2005, Journal of food protection.

[156]  M. Griffiths,et al.  A research note: the potential for transfer of Salmonella from irrigation water to tomatoes , 2004 .

[157]  A. V. Van Bruggen,et al.  Ecology of E. coli O157:H7 and Salmonella enterica in the Primary Vegetable Production Chain , 2008 .

[158]  B. Schulz,et al.  The endophytic continuum. , 2005, Mycological research.

[159]  Y. Nitzan,et al.  Die‐off of Cryptosporidium parvum in soil and wastewater effluents , 2007, Journal of applied microbiology.

[160]  J. Bassett,et al.  A risk assessment approach for fresh fruits , 2008, Journal of applied microbiology.

[161]  A. Demirci,et al.  Efficacy of pulsed UV-light for the decontamination of Escherichia coli O157:H7 and Salmonella spp. on raspberries and strawberries. , 2008, Journal of food science.

[162]  J. Barak,et al.  Role of Soil, Crop Debris, and a Plant Pathogen in Salmonella enterica Contamination of Tomato Plants , 2008, PloS one.

[163]  G. Toranzos,et al.  Enteric pathogens and soil: a short review , 2003, International microbiology : the official journal of the Spanish Society for Microbiology.

[164]  W. Zhang,et al.  Human cystic echinococcosis in two Mongolian communities in Hobukesar (China) and Bulgan (Mongolia). , 2005, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[165]  Islam Ms,et al.  Survival of Shigella flexneri in artificial aquatic environment: effects of different physicochemical stress factors. , 1996 .

[166]  In-Jung Lee,et al.  Growth promotion of red pepper plug seedlings and the production of gibberellins by Bacillus cereus, Bacillus macroides and Bacillus pumilus , 2004, Biotechnology Letters.

[167]  M. Doyle,et al.  Survival of enterohemorrhagic Escherichia coli O157:H7 in water. , 1998, Journal of food protection.

[168]  J. Vestal,et al.  Survival of microorganisms in smectite clays: implications for Martian exobiology. , 1992, Icarus.

[169]  J. Morábito,et al.  Effects on crops of irrigation with treated municipal wastewaters. , 2002, Water science and technology : a journal of the International Association on Water Pollution Research.

[170]  Nurul A. Akhand,et al.  Using MACRO to Simulate Liquid Sewage Biosolid Transport to Tile Drains for Several Land Application Methods , 2008 .

[171]  M. Peck,et al.  Clostridium botulinum and the safety of minimally heated, chilled foods: an emerging issue? , 2006, Journal of applied microbiology.

[172]  R. Bennett,et al.  Microbial evaluation of selected fresh produce obtained at retail markets. , 2002, Journal of food protection.

[173]  A. Caprioli,et al.  Enteroaggregative Escherichia coli associated with a foodborne outbreak of gastroenteritis. , 2008, Journal of medical microbiology.

[174]  Dong Sun Lee,et al.  Characterization of Bacillus cereus isolates from raw soybean sprouts. , 2004, Journal of food protection.

[175]  P. Mead,et al.  Salmonella infections associated with mung bean sprouts: epidemiological and environmental investigations , 2008, Epidemiology and Infection.

[176]  Jeri D. Barak,et al.  Salmonella enterica Virulence Genes Are Required for Bacterial Attachment to Plant Tissue , 2005, Applied and Environmental Microbiology.

[177]  O. Ozçakir [Viable but non-culturable form of bacteria]. , 2007, Mikrobiyoloji bulteni.

[178]  David G. E. Smith,et al.  Differences in Levels of Secreted Locus of Enterocyte Effacement Proteins between Human Disease-Associated and Bovine Escherichia coli O157 , 2001, Infection and Immunity.

[179]  D. Gordon,et al.  Coliform dynamics and the implications for source tracking. , 2004, Environmental microbiology.

[180]  K. Seo,et al.  Attachment of Escherichia coli O157:H7 to lettuce leaf surface and bacterial viability in response to chlorine treatment as demonstrated by using confocal scanning laser microscopy. , 1999, Journal of food protection.

[181]  F. Diez-Gonzalez,et al.  Association of farm management practices with risk of Escherichia coli contamination in pre-harvest produce grown in Minnesota and Wisconsin. , 2007, International journal of food microbiology.

[182]  F. Kasuga,et al.  Enterohemorrhagic Escherichia coli O157:H7 Present in Radish Sprouts , 1998, Applied and Environmental Microbiology.

[183]  James R. Johnson,et al.  The genetic structure of Escherichia coli populations in primary and secondary habitats. , 2002, Microbiology.

[184]  Gideon Oron,et al.  Poliovirus distribution in the soil-plant system under reuse of secondary wastewater , 1995 .

[185]  K. Scherer,et al.  Stress response of Campylobacter spp. and its role in food processing. , 2006, Journal of veterinary medicine. B, Infectious diseases and veterinary public health.

[186]  G. McFeters,et al.  Survival and injury of Escherichia coli, Campylobacter jejuni, and Yersinia enterocolitica in stream water. , 1991, Canadian journal of microbiology.

[187]  J. Bower Foodborne diseases: Shiga toxin producing E. coli (STEC). , 1999, The Pediatric infectious disease journal.

[188]  J. M. Dow,et al.  Bacterial Lipopolysaccharides And Plant—pathogen Interactions , 2004, European Journal of Plant Pathology.

[189]  R. Armon,et al.  Bacteriophage ecology in a small community sewer system related to their indicative role in sewage pollution of drinking water. , 2007, Environmental microbiology.

[190]  R. Etinger-Tulczynska,et al.  Distribution of bacteria within the tissue of healthy tomatoes. , 1963, Applied microbiology.

[191]  S. Pillai,et al.  Survival of salmonella transformed to express green fluorescent protein on Italian parsley as affected by processing and storage. , 2005, Journal of food protection.

[192]  M. Hutchison,et al.  Analyses of Livestock Production, Waste Storage, and Pathogen Levels and Prevalences in Farm Manures , 2005, Applied and Environmental Microbiology.

[193]  R. Mandrell,et al.  Escherichia coli O157:H7 survival and growth on lettuce is altered by the presence of epiphytic bacteria. , 2006, Journal of food protection.

[194]  M. Doyle,et al.  Fate of Escherichia coli O157:H7 in Manure-Amended Soil , 2002, Applied and Environmental Microbiology.

[195]  M. Hutchison,et al.  The air‐borne distribution of zoonotic agents from livestock waste spreading and microbiological risk to fresh produce from contaminated irrigation sources , 2008, Journal of applied microbiology.

[196]  C. Gerba,et al.  Comparative Inactivation of Enteroviruses and Adenovirus 2 by UV Light , 2002, Applied and Environmental Microbiology.

[197]  J. Karns,et al.  Persistence of Escherichia coli O157:H7 in soil and on plant roots. , 2002, Environmental microbiology.

[198]  S. Quessy,et al.  Persistence of Escherichia coli and Salmonella in surface soil following application of liquid hog manure for production of pickling cucumbers. , 2005, Journal of food protection.

[199]  K. Teschke,et al.  Potential for Increased Human Foodborne Exposure to PCDD/F When Recycling Sewage Sludge on Agricultural Land , 2004, Environmental health perspectives.

[200]  Pierre Servais,et al.  Fecal bacteria in the rivers of the Seine drainage network (France): sources, fate and modelling. , 2007, The Science of the total environment.

[201]  J. Cooper,et al.  Control of enteric pathogens in ready‐to‐eat vegetable crops in organic and ‘low input’ production systems: a HACCP‐based approach , 2008, Journal of applied microbiology.

[202]  R. Sleator,et al.  High-pressure processing--effects on microbial food safety and food quality. , 2008, FEMS microbiology letters.

[203]  R. Brackett,et al.  Survival of Salmonellae on and in Tomato Plants from the Time of Inoculation at Flowering and Early Stages of Fruit Development through Fruit Ripening , 2001, Applied and Environmental Microbiology.

[204]  R. Tauxe,et al.  Salmonella surveillance: a global survey of public health serotyping , 2002, Epidemiology and Infection.

[205]  Valerie J. Harwood,et al.  Performance, Design, and Analysis in Microbial Source Tracking Studies , 2007, Applied and Environmental Microbiology.

[206]  Daniel B. Oerther,et al.  Identifying fecal sources in a selected catchment reach using multiple source-tracking tools. , 2007, Journal of environmental quality.

[207]  R. Janssen,et al.  Host-Pathogen Interactions in Campylobacter Infections: the Host Perspective , 2008, Clinical Microbiology Reviews.

[208]  J. Corbo,et al.  β-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other Gram-negative bacteria , 1995 .

[209]  M. Koopmans Progress in understanding norovirus epidemiology , 2008, Current opinion in infectious diseases.

[210]  L. Beuchat,et al.  Evaluation of gaseous chlorine dioxide as a sanitizer for killing Salmonella, Escherichia coli O157:H7, Listeria monocytogenes, and yeasts and molds on fresh and fresh-cut produce. , 2005, Journal of food protection.

[211]  P. Bremer,et al.  Numbers and transported state of Escherichia coli in runoff direct from fresh cowpats under simulated rainfall * , 2006, Letters in applied microbiology.

[212]  S. Langton,et al.  Survival of poliovirus on soft fruit and salad vegetables. , 2001, Journal of food protection.

[213]  C. Siddons,et al.  A fifteen month study of Escherichia coli O157:H7 in a dairy herd , 1997, Epidemiology and Infection.

[214]  C. Turner The thermal inactivation of E. coli in straw and pig manure. , 2002, Bioresource technology.

[215]  D. Gordon,et al.  The influence of host dynamics on the clonal composition of Escherichia coli populations. , 2002, Environmental microbiology.

[216]  A. Sulakvelidze,et al.  Examination of bacteriophage as a biocontrol method for salmonella on fresh-cut fruit: a model study. , 2001, Journal of food protection.

[217]  R D Ellender,et al.  Phenotypic library-based microbial source tracking methods: efficacy in the California collaborative study. , 2003, Journal of water and health.

[218]  P. B. Lynch,et al.  Seasonal effects on the survival characteristics of Salmonella Typhimurium and Salmonella Derby in pig slurry during storage , 2007, Journal of applied microbiology.

[219]  J. Culley,et al.  Bacteriological Quality of Surface and Subsurface Runoff from Manured Sandy Clay Loam Soil 1 , 1982 .

[220]  M. N. Rojas-Valencia,et al.  Ozonation by-products issued from the destruction of microorganisms present in wastewaters treated for reuse. , 2004, Water science and technology : a journal of the International Association on Water Pollution Research.

[221]  L. Piddock,et al.  Antibiotics in media for isolation of Campylobacter spp. do not enhance resistance. , 2004, The Journal of antimicrobial chemotherapy.

[222]  J. S. Santo Domingo,et al.  Survival of Salmonella Species in River Water , 2000, Current Microbiology.

[223]  R. Moreira,et al.  Electron-beam irradiation of fresh broccoli heads (Brassica oleracea L. italica) , 2008 .

[224]  M. E. Olson,et al.  Prevalence of Giardia and Cryptosporidium and characterization of Cryptosporidium spp. isolated from wildlife, human, and agricultural sources in the North Saskatchewan River Basin in Alberta, Canada. , 2002, Canadian journal of microbiology.

[225]  Keith Warriner,et al.  Interactions of Escherichia coli O157:H7, Salmonella typhimurium and Listeria monocytogenes plants cultivated in a gnotobiotic system. , 2005, International journal of food microbiology.

[226]  P. Millner,et al.  Factors affecting compost tea as a potential source of Escherichia coli and Salmonella on fresh produce. , 2007, Journal of food protection.

[227]  Kyle R Mankin,et al.  Source specific fecal bacteria modeling using soil and water assessment tool model. , 2009, Bioresource technology.

[228]  P. Henderson,et al.  The gusBC Genes of Escherichia coli Encode a Glucuronide Transport System , 2005, Journal of bacteriology.

[229]  P. Skandamis,et al.  Risk profiles of pork and poultry meat and risk ratings of various pathogen/product combinations. , 2008, International journal of food microbiology.

[230]  S. Dixit,et al.  Diversity analysis of commensal porcine Escherichia coli - associations between genotypes and habitat in the porcine gastrointestinal tract. , 2004, Microbiology.

[231]  R. Robins-Browne The rise and rise of enteropathogenic Escherichia coli. , 2007, South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde.

[232]  A. Charkowski,et al.  The role of cellulose and O-antigen capsule in the colonization of plants by Salmonella enterica. , 2007, Molecular plant-microbe interactions : MPMI.

[233]  C. Gerba Virus survival and transplant in groundwater, (Volume 24) , 1999, Journal of Industrial Microbiology and Biotechnology.

[234]  J. Farber,et al.  Foodborne outbreaks in Canada linked to produce. , 2001, Journal of food protection.

[235]  S. Baloda,et al.  Persistence of a Salmonella entericaSerovar Typhimurium DT12 Clone in a Piggery and in Agricultural Soil Amended with Salmonella-Contaminated Slurry , 2001, Applied and Environmental Microbiology.

[236]  C. Nakatsu,et al.  Comparison of genotypic-based microbial source tracking methods requiring a host origin database. , 2003, Journal of water and health.

[237]  S. O'Brien,et al.  General outbreaks of infectious intestinal disease linked with salad vegetables and fruit, England and Wales, 1992-2000. , 2002, Communicable disease and public health.

[238]  C. Cassar,et al.  Intestinal carriage of verocytotoxigenic Escherichia coli O157, Salmonella, thermophilic Campylobacter and Yersinia enterocolitica, in cattle, sheep and pigs at slaughter in Great Britain during 2003 , 2007, Epidemiology and Infection.

[239]  B. Niemira Relative efficacy of sodium hypochlorite wash versus irradiation to inactivate Escherichia coli O157:H7 internalized in leaves of Romaine lettuce and baby spinach. , 2007, Journal of food protection.

[240]  R. Tauxe,et al.  Fresh produce: a growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997. , 2004, Journal of food protection.

[241]  K. Warriner,et al.  Inactivation of human pathogens and spoilage bacteria on the surface and internalized within fresh produce by using a combination of ultraviolet light and hydrogen peroxide , 2008, Journal of applied microbiology.

[242]  M. Tajkarimi,et al.  Impact of Vacuum Cooling on Escherichia coli O157:H7 Infiltration into Lettuce Tissue , 2008, Applied and Environmental Microbiology.

[243]  M. Sinclair,et al.  Comparative survival of enteric indicators, E. coli and somatic and F-RNA bacteriophages on turf-grass irrigated with recycled water. , 2008, Water Science and Technology.

[244]  H. Korkeala,et al.  Efficacy of disinfectants to reduce Listeria monocytogenes on precut iceberg lettuce. , 2006, Journal of food protection.

[245]  B. Woodbury,et al.  Incidence and persistence of zoonotic bacterial and protozoan pathogens in a beef cattle feedlot runoff control vegetative treatment system. , 2007, Journal of environmental quality.

[246]  V. Lazarova,et al.  Technical and sanitary aspects of wastewater disinfection by UV irradiation for landscape irrigation. , 2004, Water science and technology : a journal of the International Association on Water Pollution Research.

[247]  E. Russek-Cohen,et al.  Soil and plant effects on microbial community structure. , 2002, Canadian journal of microbiology.

[248]  A. A. Visser,et al.  Quantification of contamination of lettuce by GFP-expressing Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium. , 2007, Food microbiology.

[249]  M. Doyle,et al.  Summer meeting 2007 – the problems with fresh produce: an overview , 2008, Journal of applied microbiology.

[250]  M. Férréol,et al.  Occurrence and persistence of bacterial and viral faecal indicators in wastewater biofilms. , 2007, Water science and technology : a journal of the International Association on Water Pollution Research.

[251]  Saied Mostaghimi,et al.  Transport Of Fecal Bacteria From Poultry Litter and Cattle Manures Applied to Pastureland , 2006 .

[252]  K. Itoh,et al.  Influence of inoculation method, spot inoculation site, and inoculation size on the efficacy of acidic electrolyzed water against pathogens on lettuce. , 2003, Journal of food protection.

[253]  K. Kniel,et al.  UV light inactivation of hepatitis A virus, Aichi virus, and feline calicivirus on strawberries, green onions, and lettuce. , 2008, Journal of food protection.

[254]  T. Besser,et al.  A longitudinal study of Escherichia coli O157 in fourteen cattle herds , 1997, Epidemiology and Infection.

[255]  T. Trout,et al.  Application of alternative fumigants through drip irrigation systems. , 2002, Phytopathology.

[256]  A. Roig-Sagués,et al.  Inactivation by ultrahigh-pressure homogenization of Escherichia coli strains inoculated into orange juice. , 2006, Journal of food protection.

[257]  R. Mandrell,et al.  Association of Escherichia coli O157:H7 with preharvest leaf lettuce upon exposure to contaminated irrigation water. , 2002, Journal of food protection.

[258]  G. Barker,et al.  Germination and growth from spores: variability and uncertainty in the assessment of food borne hazards. , 2005, International journal of food microbiology.

[259]  C. Palmer,et al.  The public health and clinical significance of Giardia and Cryptosporidium in domestic animals , 2007, The Veterinary Journal.

[260]  M. Chinnan,et al.  Consumer acceptance of fresh-cut iceberg lettuce treated with 2% hydrogen peroxide and mild heat. , 2002, Journal of food protection.

[261]  J. Osek,et al.  Shiga toxin-producing E. coli - the actual state of knowledge. , 2007 .

[262]  R. Jonge,et al.  Survival of Campylobacter jejuni Strains from Different Origins Under Oxidative Stress Conditions: Effect of Temperature , 2008, Current Microbiology.

[263]  D. Schulze‐Makuch,et al.  Microbial survival rates of Escherichia coli and Deinococcus radiodurans under low temperature, low pressure, and UV-Irradiation conditions, and their relevance to possible Martian life. , 2006, Astrobiology.

[264]  S.R. Smith,et al.  Influence of soil type, moisture content and biosolids application on the fate of Escherichia coli in agricultural soil under controlled laboratory conditions , 2007, Journal of applied microbiology.

[265]  Eric D Mintz,et al.  Concurrent outbreaks of Shigella sonnei and enterotoxigenic Escherichia coli infections associated with parsley: implications for surveillance and control of foodborne illness. , 2003, Journal of food protection.

[266]  K. Killham,et al.  Survival of Escherichia coli O157:H7 in private drinking water wells: influences of protozoan grazing and elevated copper concentrations. , 2002, FEMS microbiology letters.

[267]  S F Tyrrel,et al.  Overland flow transport of pathogens from agricultural land receiving faecal wastes , 2003, Journal of applied microbiology.

[268]  T. Whittam Clonal dynamics ofEscherichia coli in its natural habitat , 2006, Antonie van Leeuwenhoek.

[269]  S. Phatak,et al.  Fate of Salmonella enterica Serovar Typhimurium on Carrots and Radishes Grown in Fields Treated with Contaminated Manure Composts or Irrigation Water , 2004, Applied and Environmental Microbiology.

[270]  M. Dickinson,et al.  Internalization of Human Pathogens within Growing Salad Vegetables , 2003, Biotechnology & genetic engineering reviews.

[271]  R. Mandrell,et al.  Colonization of Arabidopsis thaliana with Salmonella enterica and Enterohemorrhagic Escherichia coli O157:H7 and Competition by Enterobacter asburiae , 2003, Applied and Environmental Microbiology.