Segmental distribution of nucleotides in the DNA of bacteriophage lambda.

DNA molecules isolated from phage λ were fragmented by shear and fractionated in various ways, mainly with respect to buoyant density of mercury complexes in Cs2SO4. Six segments of different composition were found, ranging in G + C content from 37 to 57 mole %. The lengths and left-to-right sequence of segments were determined. The segments are reasonably homogeneous internally and the boundaries between segments rather sharp.

[1]  E. Freese.  On the evolution of the base composition of DNA , 1962 .

[2]  Ross B. Inman,et al.  A denaturation map of the λ phage DNA molecule determined by electron microscopy , 1966 .

[3]  A. Campbell,et al.  The relationship between heritable physical and genetic properties of selected gal− and gal+ transducing λdg , 1966 .

[4]  N. Davidson,et al.  Separation of Deoxyribonucleic Acids by Hg(II) Binding and Cs2SO4 Density-Gradient Centrifugation* , 1965 .

[5]  L. Caro.  THE MOLECULAR WEIGHT OF LAMBDA DNA. , 1965, Virology.

[6]  J. Speyer.  Mutagenic DNA polymerase. , 1965, Biochemical and biophysical research communications.

[7]  J. Smith,et al.  The structure of ribonucleic acid. I. Cyclic nucleotides produced by ribonuclease and by alkaline hydrolysis. , 1952, The Biochemical journal.

[8]  D. Hogness,et al.  Breakage of λdg DNA: Chemical and genetic characterization of each isolated half-molecule , 1964 .

[9]  A. D. Hershey,et al.  A relative molecular weight series derived from the nucleic acid of bacteriophage T2. , 1961, Journal of molecular biology.

[10]  A. D. Hershey,et al.  Sedimentation rate as a measure of molecular weight of DNA. , 1963, Biophysical journal.

[11]  C. I. Davern,et al.  Preparative density-gradient centrifugation of the molecular halves of lambda DNA , 1965 .

[12]  A. D. Hershey,et al.  Sedimentation Coefficient and Fragility under Hydrodynamic Shear as Measures of Molecular Weight of the DNA of Phage T5. , 1962, Biophysical journal.

[13]  S. Falkow,et al.  Spectral analysis of the intramolecular heterogeneity of λ DNA , 1967 .

[14]  H. Bond,et al.  Density-Gradient centrifugation of DNA in a fixed-angle rotor: A higher order of resolution , 1966 .

[15]  C. A. Thomas,et al.  NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES. , 1965, Journal of molecular biology.

[16]  R. Rownd,et al.  Molecular nature of the drug-resistance factors of the Enterobacteriaceae. , 1966, Journal of molecular biology.

[17]  F. Stahl,et al.  Circular genetic maps , 1967, Journal of cellular physiology.

[18]  J. Hurwitz,et al.  Role of DNA in RNA synthesis. XI. Selective transcription of gamma DNA segments in vitro by RNA polymerase of Escherichia coli. , 1967, Journal of molecular biology.