Web-based autonomous learning environment is the circumstances and conditions in the process of learning activities with the introduction of the network factors, its basic elements not only include the support of courses contents, instructional strategies, teaching mode and resources, but also cover instruction design, management, evaluation and other areas. The e-Portfolio (Electronic Learning Portfolio) is a collection of the learner’s experiences and achievements during a period of educational activity, which has advantages in dynamic tracking, recording, collection, evaluation ,management and other aspects, the interposition of E-Portfolio provides new thought of the construction of web-based Autonomous Learning Environment. In this article, on the base of studying web-based autonomous learning and E-Portfolio, the author mainly discussed the construction of e-Portfolio based autonomous learning environment in E-learning.