Titanium nanoparticles (TiO2)/graphene oxide nanosheets (GO): an electrochemical sensing platform for the sensitive and simultaneous determination of benzocaine in the presence of antipyrine.

An effective electrochemical sensing platform for the simultaneous determination of benzocaine (BEN) and antipyrine (ANT) based upon titanium dioxide nanoparticle (TiO2)/graphene oxide nanosheet (GO) bulk modified carbon paste electrodes (TiO2-GO/CPE) is reported. The TiO2-GO/CPE electrochemical sensing platform is found to exhibit linear ranges from 1.0 × 10-6 to 1.0 × 10-4 M and 1.2 × 10-8 to 8.0 × 10-5 M for BEN and ANT, respectively. The TiO2-GO/CPE sensor is explored towards the analysis of BEN and ANT in oral fluid (saliva) and pharmaceutical products. The synergy between the graphene oxide nanosheets and titanium dioxide nanoparticles results in a dramatic enhancement in the sensitivity of the sensor through a combination of increased surface area and improved electron transfer kinetics compared to other electrode alternatives. The fabricated TiO2-GO/CPE exhibits high sensitivity and good stability towards the sensing of BEN and ANT and has the potential to be utilised as a clinical assay and QA in pharmaceutical products.

[1]  C. Banks,et al.  Highly Selective Sensing Platform Utilizing Graphene Oxide and Multiwalled Carbon Nanotubes for the Sensitive Determination of Tramadol in the Presence of Co-Formulated Drugs , 2017 .

[2]  Nageh K. Allam,et al.  Novel MWCNTs/graphene oxide/pyrogallol composite with enhanced sensitivity for biosensing applications. , 2017, Biosensors & bioelectronics.

[3]  N. Zhang,et al.  Graphene and its derivatives as versatile templates for materials synthesis and functional applications. , 2017, Nanoscale.

[4]  N. Zhang,et al.  Metal-free, robust, and regenerable 3D graphene–organics aerogel with high and stable photosensitization efficiency , 2017 .

[5]  H. Merey.  Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine , 2016 .

[6]  N. Zhang,et al.  Waltzing with the Versatile Platform of Graphene to Synthesize Composite Photocatalysts. , 2015, Chemical reviews.

[7]  D. A. Brownson,et al.  The Handbook of Graphene Electrochemistry , 2014 .

[8]  M. Taleb,et al.  Evaluation and Management of Acquired Methemoglobinemia Associated with Topical Benzocaine Use , 2013, American Journal of Cardiovascular Drugs.

[9]  Stephanie Chung,et al.  The FDA drug safety surveillance program: adverse event reporting trends. , 2011, Archives of internal medicine.

[10]  J. Barek,et al.  Determination of Benzocaine Using HPLC and FIA with Amperometric Detection on a Carbon Paste Electrode , 2011 .

[11]  Bo Yan,et al.  One step hydrothermal synthesis of TiO2-reduced graphene oxide sheets , 2011 .

[12]  Xianzhi Fu,et al.  TiO2-graphene nanocomposites for gas-phase photocatalytic degradation of volatile aromatic pollutant: is TiO2-graphene truly different from other TiO2-carbon composite materials? , 2010, ACS nano.

[13]  S. Pichini,et al.  High performance liquid chromatography-diode array and electrospray-mass spectrometry analysis of vardenafil, sildenafil, tadalafil, testosterone and local anesthetics in cosmetic creams sold on the Internet web sites. , 2009, Journal of pharmaceutical and biomedical analysis.

[14]  R. Compton,et al.  Using Capsaicin Modified Multiwalled Carbon Nanotube Based Electrodes and p-Chloranil Modified Carbon Paste Electrodes for the Determination of Amines: Application to Benzocaine and Lidocaine , 2008 .

[15]  Xiaobo Chen,et al.  Titanium dioxide nanomaterials: synthesis, properties, modifications, and applications. , 2007, Chemical reviews.

[16]  S. Stankovich,et al.  Synthesis of graphene-based nanosheets via chemical reduction of exfoliated graphite oxide , 2007 .

[17]  P. Scherpenisse,et al.  Determination of residues of tricaine in fish using liquid chromatography tandem mass spectrometry. , 2007, Analytica chimica acta.

[18]  J. Barbosa-Filho,et al.  Anti-inflammatory activity of alkaloids: a twenty-century review , 2006 .

[19]  J. M. Suñé-Negre,et al.  A new validated method for the simultaneous determination of benzocaine, propylparaben and benzyl alcohol in a bioadhesive gel by HPLC. , 2005, Journal of pharmaceutical and biomedical analysis.

[20]  M. Srikanth,et al.  Topical Benzocaine (Hurricaine®) Induced Methemoglobinemia during Endoscopic Procedures in Gastric Bypass Patients , 2005, Obesity surgery.

[21]  G. Duan,et al.  Simultaneous determination of ropivacaine and antipyrine by high performance liquid chromatography and its application to the in vitro transplacental study. , 2004, Biomedical chromatography : BMC.

[22]  Michael R. Cohen,et al.  Reported adverse event cases of methemoglobinemia associated with benzocaine products. , 2004, Archives of internal medicine.

[23]  M. Grant,et al.  Determination of diminazene aceturate in pharmaceutical formulations by HPLC and identification of related substances by LC/MS. , 2002, Journal of pharmaceutical and biomedical analysis.

[24]  A. Olivieri,et al.  SIMULTANEOUS MULTIVARIATE SPECTROPHOTOMETRIC ANALYSIS OF EAR DROPS CONTAINING A TERNARY MIXTURE OF ANTIPYRINE, SULFATHIAZOLE, AND RIVANOL , 2001 .

[25]  A. Olivieri,et al.  Simultaneous spectrophotometric-multivariate calibration determination of several components of ophthalmic solutions: phenylephrine, chloramphenicol, antipyrine, methylparaben and thimerosal. , 2000, Talanta.

[26]  M. Polášek,et al.  Determination of procaine, benzocaine and tetracaine by sequential injection analysis with permanganate-induced chemiluminescence detection. , 2000, Talanta.

[27]  M. Moeder,et al.  Solid-phase microextraction-gas chromatography-mass spectrometry of biologically active substances in water samples. , 2000, Journal of chromatography. A.

[28]  T. Hubert,et al.  Liquid chromatographic determination of benzocaine and N-acetylbenzocaine in the edible fillet tissue from rainbow trout. , 1999, Journal of chromatography. A.

[29]  I. Wilson.  Thin-layer chromatography: a neglected technique. , 1996, Therapeutic drug monitoring.

[30]  D. Perrett,et al.  Rapid determination of drugs in biofluids by capillary electrophoresis. Measurement of antipyrine in saliva for pharmacokinetic studies. , 1995, Journal of chromatography. A.

[31]  U. Hofmann,et al.  Highly sensitive and specific gas chromatographic-tandem mass spectrometric method for the determination of trace amounts of antipyrine metabolites in biological material. , 1995, Journal of chromatography. B, Biomedical applications.

[32]  W. Baeyens,et al.  Chemiluminescence determination of some local anaesthetics , 1995 .

[33]  S. Pinzauti,et al.  Simultaneous UV spectrophotometric determination of procaine hydrochloride and phenazone in an otic formulation , 1990 .

[34]  I. Shukrallah,et al.  Comparative Study of Some Methods for the Determination of the Analgesic Anti‐Inflammatory Compound “IS4”: 4‐(Anisylideneamino) Antipyrine , 1986 .

[35]  G. Ceschel,et al.  Design and in vitro-in vivo evaluation of a bi-layered tablet containing benzocaine for local buccal administration , 2004 .

[36]  G. Rücker,et al.  Quantitative determination of analgesic mixture of phenazone, phenacetin and caffeine in the presence of some of their degradation products. , 1991, Journal of pharmaceutical and biomedical analysis.