Characterization of a CD11c-reactive monoclonal antibody (HC1/1) obtained by immunizing with phorbol ester differentiated U937 cells.

A mouse monoclonal antibody (HC1/1) specific for a differentiation marker of human monocytes and granulocytes has been generated by using as immunogen the monocytic cell line U937 differentiated with phorbol esters. This differentiation antigen has been characterized as the p150/95 member of the CD11 family of glycoproteins by cellular distribution studies, immunoprecipitation and competition experiments with MAb 3.9 specific for the CD11c antigen. Immuno-alkaline phosphatase staining of normal tissue sections with the HC1/1 MAb demonstrated that the CD11c antigen is a useful macrophage marker. Furthermore, the MAb HC1/1 stains specifically populations of macrophages on skin and lymph node sections from different pathologies such as Sarcoidosis, Granuloma annulare, Lepromatous leprosy and Toxoplasmosis.

[1]  A. Boyd,et al.  Production and characterization of a monoclonal antibody (A1-3) that binds selectively to activated monocytes and inhibits monocyte procoagulant activity. , 1986, Journal of immunology.

[2]  C. Y. Wang,et al.  The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemia. , 1985, Blood.

[3]  E. Bröcker,et al.  A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. , 1985, Journal of immunology.

[4]  J. Weiel,et al.  Biochemical models of gamma-interferon action: altered expression of transferrin receptors on murine peritoneal macrophages after treatment in vitro with PMA or A23187. , 1985, Journal of immunology.

[5]  F. Sánchez‐Madrid,et al.  A human leukocyte differentiation antigen family with distinct alpha- subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule , 1983, The Journal of experimental medicine.

[6]  R. Steinman,et al.  Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages , 1983, The Journal of experimental medicine.

[7]  F. Sánchez‐Madrid,et al.  Stable hamster-mouse hybridomas producing IgG and IgM hamster monoclonal antibodies of defined specificity. , 1983, Journal of immunology.

[8]  R A Laskey,et al.  Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film , 1977, FEBS letters.

[9]  E. Unanue.  Secretory function of mononuclear phagocytes: a review. , 1976, The American journal of pathology.

[10]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[11]  H. RINDERKNECHT,et al.  Ultra-Rapid Fluorescent Labelling of Proteins , 1962, Nature.

[12]  R. Furth.  Current view on the mononuclear phagocyte system , 1982 .

[13]  R. B. Narayanan,et al.  The origin, morphology, and function of epithelioid cells. , 1982, Immunobiology.