Cloning of plasmid genes encoding resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus CH34

A 238-kilobase-pair plasmid, pMOL30, confers resistance to cadmium, zinc, and cobalt salts in Alcaligenes eutrophus CH34. After Tn5 mutagenesis, restriction nuclease analysis, and Southern DNA-DNA hybridization, a 9.1-kilobase-pair EcoRI fragment was found to harbor all of these resistance properties and was cloned into the broad-host-range hybrid plasmid pRK290. When transferred to a plasmid-free derivative of CH34, the hybrid plasmid conferred the same degree of resistance as the parent plasmid pMOL30. In two other Alcaligenes strains, the hybrid plasmid was expressed, but to a lower degree than in CH34 derivatives.

[1]  M. Mergeay,et al.  Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals , 1985, Journal of bacteriology.

[2]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[3]  M. Mergeay,et al.  Chromosome transfer and R-prime plasmid formation mediated by plasmid pULB113 (RP4::mini-Mu) in Alcaligenes eutrophus CH34 and Pseudomonas fluorescens 6.2 , 1983, Journal of bacteriology.

[4]  A. Pühler,et al.  Vector Plasmids for in-Vivo and in-Vitro Manipulations of Gram-Negative Bacteria , 1983 .

[5]  A. Pühler,et al.  A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria , 1983, Bio/Technology.

[6]  S. T. Liu,et al.  Rapid procedure for detection and isolation of large and small plasmids , 1981, Journal of bacteriology.

[7]  H. Biebl,et al.  Isolation of Members of the Family Rhodospirillaceae , 1981 .

[8]  G. Ditta,et al.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[9]  T. Foster,et al.  Transposon A-generated mutations in the mercuric resistance genes of plasmid R100-1 , 1979, Journal of bacteriology.

[10]  T. Miki,et al.  Hypersensitivity to Hg2+ and hyperbinding activity associated with cloned fragments of the mercurial resistance operon of plasmid NR1 , 1979, Journal of bacteriology.

[11]  M. Okazaki,et al.  A NEW SPECIES OF HYDROGEN-UTILIZING BACTERIUM , 1979 .

[12]  F. Bolivar,et al.  Plasmids of Escherichia coli as cloning vectors. , 1979, Methods in enzymology.

[13]  R. H. Olsen,et al.  Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5 , 1978, Journal of bacteriology.

[14]  M. Mergeay,et al.  Extrachromosomal inheritance controlling resistance to cadmium, cobalt, copper and zinc ions: evidence from curing in a Pseudomonas [proceedings]. , 1978, Archives internationales de physiologie et de biochimie.

[15]  P Berg,et al.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. , 1977, Journal of molecular biology.

[16]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[17]  M Mandel,et al.  Calcium-dependent bacteriophage DNA infection. , 1970, Journal of molecular biology.

[18]  H. Echols,et al.  Genetic control of repression of alkaline phosphatase in E. coli. , 1961, Journal of molecular biology.