Metabolite profiling is the multiparallel relative quantification of a mixture of compounds or compound classes using chromatography and universal detection technologies (GC-MS, LC-MS). In this respect it is an extension of classical single-target methods from which it can be distinguished by its broader view on profiling major biochemical events. This broader scope of analysis outweighs the disadvantages by making compromises in method development and the reduced accuracy for specific metabolites. This chapter exemplifies the strategies in metabolite profiling of polar compounds by gas chromatography-mass spectrometry (GC-MS). It gives experimental details on the basic steps: harvest, homogenization, extraction, fractionation, concentration, derivatization, data acquisition, raw data processing and result data tranformation.
[1]
S. Stein.
An integrated method for spectrum extraction and compound identification from gas chromatography/mass spectrometry data
,
1999
.
[2]
I. Krull,et al.
Analytical method development and validation for the academic researcher
,
1999
.
[3]
O. Fiehn.
Metabolomics – the link between genotypes and phenotypes
,
2004,
Plant Molecular Biology.
[4]
Mark P Molloy,et al.
Overcoming technical variation and biological variation in quantitative proteomics
,
2003,
Proteomics.
[5]
O. Fiehn,et al.
Process for the integrated extraction, identification and quantification of metabolites, proteins and RNA to reveal their co‐regulation in biochemical networks
,
2004,
Proteomics.