Functional Genomic Study on Atrial Fibrillation Using cDNA Microarray and Two‐Dimensional Protein Electrophoresis Techniques and Identification of the Myosin Regulatory Light Chain Isoform Reprogramming in Atrial Fibrillation

Introduction: Functional and structural changes of atrial tissue occur during the natural course of atrial fibrillation (AF), and these changes may contribute to further AF. We investigated the changes in AF tissue using cDNA microarray and two‐dimensional protein electrophoresis techniques.

[1]  G. Dorn,et al.  Effects of total replacement of atrial myosin light chain-2 with the ventricular isoform in atrial myocytes of transgenic mice. , 1998, Circulation.

[2]  D. Botstein,et al.  Cluster analysis and display of genome-wide expression patterns. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[3]  J. Winer,et al.  Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. , 1999, Analytical biochemistry.

[4]  R. Moss,et al.  Altered kinetics of contraction of mouse atrial myocytes expressing ventricular myosin regulatory light chain. , 1999, American journal of physiology. Heart and circulatory physiology.

[5]  M. Allessie,et al.  Electrical, contractile and structural remodeling during atrial fibrillation. , 2002, Cardiovascular research.

[6]  S. Lee,et al.  A strategy for identification and quantitation of phosphopeptides by liquid chromatography/tandem mass spectrometry. , 2000, Analytical biochemistry.

[7]  J Clémenty,et al.  Spontaneous initiation of atrial fibrillation by ectopic beats originating in the pulmonary veins. , 1998, The New England journal of medicine.

[8]  M. Siddiqui,et al.  Expression of Ventricular‐Type Myosin Light Chain Messenger RNA in Spontaneously Hypertensive Rat Atria , 1988, Circulation research.

[9]  I. V. Van Gelder,et al.  Gene expression of proteins influencing the calcium homeostasis in patients with persistent and paroxysmal atrial fibrillation. , 1999, Cardiovascular research.

[10]  M. Bittner,et al.  Expression profiling using cDNA microarrays , 1999, Nature Genetics.

[11]  George C Tseng,et al.  Microarray gene expression profiles in dilated and hypertrophic cardiomyopathic end-stage heart failure. , 2002, Physiological genomics.

[12]  S. Huang,et al.  Down-regulation of L-type calcium channel and sarcoplasmic reticular Ca(2+)-ATPase mRNA in human atrial fibrillation without significant change in the mRNA of ryanodine receptor, calsequestrin and phospholamban: an insight into the mechanism of atrial electrical remodeling. , 1999, Journal of the American College of Cardiology.

[13]  Joachim Klose,et al.  Two‐dimensional electrophoresis of proteins: An updated protocol and implications for a functional analysis of the genome , 1995, Electrophoresis.

[14]  J. Cheung,et al.  Modulation of cardiac contractility by myosin light chain phosphorylation. , 1991, Medicine and science in sports and exercise.

[15]  R. Tieleman,et al.  Alterations in Gene Expression of Proteins Involved in the Calcium Handling in Patients with Atrial Fibrillation , 1999, Journal of cardiovascular electrophysiology.

[16]  R. Fleischmann,et al.  Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence. , 1995, Nature.

[17]  J. Yates,et al.  An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database , 1994, Journal of the American Society for Mass Spectrometry.

[18]  Jeremy D. Glasner,et al.  Genomic Interspecies Microarray Hybridization: Rapid Discovery of Three Thousand Genes in the Maize Endophyte,Klebsiella pneumoniae 342, by Microarray Hybridization with Escherichia coli K-12 Open Reading Frames , 2001, Applied and Environmental Microbiology.

[19]  L. Lai,et al.  Electrophysiological Mapping and Histological Examinations of the Swine Atrium with Sustained (≥24 h) Atrial Fibrillation: A Suitable Animal Model for Studying Human Atrial Fibrillation , 2003, Cardiology.

[20]  A. Goette,et al.  Regulation of angiotensin II receptor subtypes during atrial fibrillation in humans. , 2000, Circulation.

[21]  M. Allessie,et al.  Atrial fibrillation begets atrial fibrillation. A study in awake chronically instrumented goats. , 1995, Circulation.

[22]  M. Böhm,et al.  Calcium sensitivity and myosin light chain pattern of atrial and ventricular skinned cardiac fibers from patients with various kinds of cardiac disease. , 1990, Journal of molecular and cellular cardiology.

[23]  R. Bosch,et al.  Atrial L-type Ca2+-channel, β-adrenoreceptor, and 5-hydroxytryptamine type 4 receptor mRNAs in human atrial fibrillation , 2001, Basic Research in Cardiology.

[24]  L. Lai,et al.  Measurement of Funny Current (If) Channel mRNA in Human Atrial Tissue: Correlation with Left Atrial Filling Pressure and Atrial Fibrillation , 1999, Journal of cardiovascular electrophysiology.