STUDIES OF MOLECULAR INTERACTION IN ISOIONIC PROTEIN SOLUTIONS BY LIGHT-SCATTERING.

The technique of light-scattering has been extensively employed in recent years in physicochemical investigations of proteins in solution.', 2 It is useful not only for the determination of molecular weights of macromolecules but also for the measurement of their thermodynamic interaction. The excess turbidity r, arising from composition fluctuations, in a solution of two components1 is related to the chemical potential 12 and molecular weight M2 of the solute in the following manner: