Tunable axial superresolution by annular binary filters. Application to confocal microscopy

We present a set of annular binary pupil filters for increasing the axial resolving capacity of imaging systems. The filters consist of two transparent annuli of the same area. It is shown that by changing the area of the transparent regions it is possible to obtain a tunable reduction of the width of the central lobe of the axial point spread function of the imaging system. However, this reduction is accompanied by a severe increase of the strength of secondary lobes, what can make these filters not very useful when used in conventional imaging systems. That is why we propose to use these filters for apodizing confocal microscopy systems. It is shown that in this case an important reduction is achieved in the volume of the central lobe of the three-dimensional point spread function.

[1]  M Martínez-Corral,et al.  On-axis diffractional behavior of two-dimensional pupils. , 1994, Applied optics.

[2]  H. H. Hopkins,et al.  Influence of Non-uniform Amplitude on PSF , 1988 .

[3]  Stefan W. Hell,et al.  Enhancing the Axial Resolution in Far-field Light Microscopy: Two-photon 4Pi Confocal Fluorescence Microscopy , 1994 .

[4]  M J Yzuel,et al.  Polychromatic axial behavior of axial apodizing and hyperresolving filters. , 1990, Applied optics.

[5]  Z. S. Hegedus,et al.  Superresolving filters in confocally scanned imaging systems , 1986 .

[6]  A. Yoshida,et al.  Effect of aberrations on off-axis gaussian beams , 1975 .

[7]  Superresolving masks for incoherent scanning microscopy , 1993 .

[8]  Colin J. R. Sheppard,et al.  Axial behavior of pupil-plane filters , 1988 .

[9]  Zoltan S. Hegedus,et al.  Annular pupil arrays. Application to confocal scanning , 1985 .

[10]  J. Tsujiuchi IV Correction of Optical Images by Compensation of Aberrations and by Spatial Frequency Filtering , 1963 .

[11]  F. Wyrowski,et al.  Error diffusion procedure: theory and applications in optical signal processing. , 1992, Applied optics.

[12]  J Ojeda-Castañeda,et al.  Arbitrarily high focal depth with finite apertures. , 1988, Optics letters.

[13]  C. Sheppard,et al.  Image Formation in the Scanning Microscope , 1977 .

[14]  Irene A. Stegun,et al.  Handbook of Mathematical Functions. , 1966 .

[15]  Pedro Andrés,et al.  Strehl ratio with low sensitivity to spherical aberration , 1988 .

[16]  Colin J. R. Sheppard,et al.  Information capacity and resolution in an optical system , 1986 .

[17]  Colin J. R. Sheppard,et al.  Improvement of axial resolution in confocal microscopy using an annular pupil , 1991 .

[18]  M Martínez-Corral,et al.  Zero axial irradiance by annular screens with angular variation. , 1992, Applied optics.

[19]  B. Roy Frieden,et al.  On Arbitrarily Perfect Imagery with a Finite Aperture , 1969 .

[20]  María J. Yzuel,et al.  Supergaussian rings: focusing properties , 1995 .

[21]  T. Wilson,et al.  The Use of Annular Pupil Plane Filters to Tune the Imaging Properties in Confocal Microscopy , 1990 .