Superparamagnetic iron oxide nanoparticles coated with different polymers and their MRI contrast effects in the mouse brains

Abstract PEG and PEG/PEI modified superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by the thermal decomposition of iron (III) acetylacetonate (Fe(acac) 3 ) in poly (ethylene glycol) (PEG) containing poly (ethylene imine) (PEI) (0 or 0.3 g). PEG/PEI-SPIONs were coated with Tween 80 (PEG/PEI/Tween 80-SPIONs). Fourier transform infrared spectroscopy (FTIR) analyses indicated that PEG, PEG/PEI and PEG/PEI/Tween 80 were attached to the surfaces of the SPIONs. The PEG-SPIONs, PEG/PEI-SPIONs and PEG/PEI/Tween 80-SPIONs performed excellent colloidal stability in the phosphate buffered saline (PBS), and in deionized water with the mean hydrodynamic sizes of 19.5, 21.0, 24.0 nm and the zeta potentials of -5.0, 35.0, 19.0 mV, respectively. All the SPIONs showed low cytotoxicity assessed by the MTT assay. In vivo magnetic resonance imaging (MRI) of the Kunming (KM) mouse brains were performed, the PEG-SPIONs, PEG/PEI-SPIONs and PEG/PEI/Tween 80-SPIONs exhibited vascular imaging effects in bulbus olfactorius, frontal cortex, temporal, thalamus and brain stem of the mouse brains after 24 h intravenous injection of the nanoparticles. The SPIONs have potentials as MRI contrast agents in the mouse brains.

[1]  R. Jayasree,et al.  Synthesis and characterization of dextran stabilized superparamagnetic iron oxide nanoparticles for in vivo MR imaging of liver fibrosis. , 2014, Carbohydrate polymers.

[2]  R. Jaeger,et al.  Surface Modification of Poly(vinyl alcohol) by Peroxide-Initiated Grafting of a Poly(organophosphazene) , 1996 .

[3]  Seong-Gi Kim,et al.  Cerebral blood volume MRI with intravascular superparamagnetic iron oxide nanoparticles , 2013, NMR in biomedicine.

[4]  Yung-Chuan Liu,et al.  Optimum conditions for lipase immobilization on chitosan-coated Fe3O4 nanoparticles , 2012 .

[5]  Zhichuan J. Xu,et al.  Oleylamine as Both Reducing Agent and Stabilizer in a Facile Synthesis of Magnetite Nanoparticles , 2009 .

[6]  S. Sasaki,et al.  Pin-point chemical modification of RNA with diverse molecules through the functionality transfer reaction and the copper-catalyzed azide-alkyne cycloaddition reaction. , 2011, Chemical communications.

[7]  Yihang Wu,et al.  In vitro cytotoxicity of silver nanoparticles and zinc oxide nanoparticles to human epithelial colorectal adenocarcinoma (Caco-2) cells. , 2014, Mutation research.

[8]  R. Jayasree,et al.  Citrate coated iron oxide nanoparticles with enhanced relaxivity for in vivo magnetic resonance imaging of liver fibrosis. , 2014, Colloids and surfaces. B, Biointerfaces.

[9]  Y. M. Wang,et al.  Synthesis of Fe3O4 magnetic fluid used for magnetic resonance imaging and hyperthermia , 2011 .

[10]  Qiang Liu,et al.  Design of pH-responsive nanoparticles of terpolymer of poly(methacrylic acid), polysorbate 80 and starch for delivery of doxorubicin. , 2013, Colloids and surfaces. B, Biointerfaces.

[11]  Oliver T. Bruns,et al.  A highly effective, nontoxic T1 MR contrast agent based on ultrasmall PEGylated iron oxide nanoparticles. , 2009, Nano letters.

[12]  P. Subramanian,et al.  Biosynthesis of Iron oxide nanoparticles and its haematological effects on fresh water fish Oreochromis mossambicus , 2013 .

[13]  Mingyuan Gao,et al.  One-Pot Reaction to Synthesize Water-Soluble Magnetite Nanocrystals , 2004 .

[14]  T. Park,et al.  Clustered magnetite nanocrystals cross-linked with PEI for efficient siRNA delivery. , 2011, Biomacromolecules.

[15]  H. Toma,et al.  Preparation and characterization of (3-aminopropyl) triethoxysilane-coated magnetite nanoparticles , 2004 .

[16]  Taeghwan Hyeon,et al.  Large-scale synthesis of uniform and extremely small-sized iron oxide nanoparticles for high-resolution T1 magnetic resonance imaging contrast agents. , 2011, Journal of the American Chemical Society.

[17]  Rajesh Singh,et al.  Nanoparticle-based targeted drug delivery. , 2009, Experimental and molecular pathology.

[18]  E. Leite,et al.  Synthesis of colloidal magnetite nanocrystals using high molecular weight solvent , 2010 .

[19]  Raoul Kopelman,et al.  Brain cancer diagnosis and therapy with nanoplatforms. , 2006, Advanced drug delivery reviews.

[20]  Huabei Peng,et al.  Re-examination of characteristic FTIR spectrum of secondary layer in bilayer oleic acid-coated Fe3O4 nanoparticles , 2010 .

[21]  Kavitha Sunassee,et al.  Aluminium hydroxide stabilised MnFe2O4 and Fe3O4 nanoparticles as dual-modality contrasts agent for MRI and PET imaging , 2014, Biomaterials.

[22]  G. Zhai,et al.  The permeability of puerarin loaded poly(butylcyanoacrylate) nanoparticles coated with polysorbate 80 on the blood-brain barrier and its protective effect against cerebral ischemia/reperfusion injury. , 2013, Biological & pharmaceutical bulletin.

[23]  C. Bianchi,et al.  One-step synthesis and functionalization of hydroxyl-decorated magnetite nanoparticles. , 2008, Journal of colloid and interface science.

[24]  W. Cai,et al.  Monodisperse water-soluble magnetite nanoparticles prepared by polyol process for high-performance magnetic resonance imaging. , 2007, Chemical communications.

[25]  Jue Zhang,et al.  Synthesis of Well-Dispersed Aqueous-Phase Magnetite Nanoparticles and Their Metabolism as an MRI Contrast Agent for the Reticuloendothelial System , 2011 .

[26]  C. F. Mabery,et al.  Die Schwefelverbindungen im rohen Petroleum und in Petroleum‐Rückständen , 1889 .

[27]  Chandana Mohanty,et al.  Dual drug loaded superparamagnetic iron oxide nanoparticles for targeted cancer therapy. , 2010, Biomaterials.

[28]  Yunfei Sun,et al.  A comprehensive study on the synthesis and paramagnetic properties of PEG-coated Fe3O4 nanoparticles , 2014 .

[29]  C. Cho,et al.  Surface modification of iron oxide nanoparticles by biocompatible polymers for tissue imaging and targeting. , 2013, Biotechnology advances.

[30]  Xiaogang Peng,et al.  Size- and Shape-Controlled Magnetic (Cr, Mn, Fe, Co, Ni) Oxide Nanocrystals via a Simple and General Approach , 2004 .

[31]  Lingyun Feng,et al.  Preparation and magnetic properties of magnetite nanoparticles , 2012 .

[32]  M. Muhammed,et al.  Biodegradable polymeric vesicles containing magnetic nanoparticles, quantum dots and anticancer drugs for drug delivery and imaging. , 2014, Biomaterials.

[33]  J. Dai,et al.  Magnetic resonance imaging of Fe3O4@SiO2-labeled human mesenchymal stem cells in mice at 11.7 T. , 2013, Biomaterials.

[34]  Forrest M Kievit,et al.  PEI–PEG–Chitosan‐Copolymer‐Coated Iron Oxide Nanoparticles for Safe Gene Delivery: Synthesis, Complexation, and Transfection , 2009, Advanced functional materials.

[35]  H. Fan,et al.  Ultrasonic dispersion of nano TiC powders aided by Tween 80 addition , 2012 .

[36]  Baoling Zhang,et al.  Synthesis and properties of magnetite nanoparticles coated with poly(ethylene glycol) and poly(ethylene imine). , 2013, Journal of nanoscience and nanotechnology.

[37]  Baoling Zhang,et al.  Superparamagnetic iron oxide nanoparticles prepared by using an improved polyol method , 2013 .

[38]  Benjamin R. Jarrett,et al.  Modulation of T2 relaxation time by light-induced, reversible aggregation of magnetic nanoparticles. , 2010, Journal of the American Chemical Society.

[39]  Mingyuan Gao,et al.  Receptor-mediated delivery of magnetic nanoparticles across the blood-brain barrier. , 2012, ACS nano.