The effect of Hsa_circ_0001451 in clear cell renal cell carcinoma cells and its relationship with clinicopathological features

Purpose: Circular RNAs (circRNAs), are a large class of RNAs that from a covalently closed continuous loop and have recently showed huge capabilities as gene regulators in mammals. Although Hsa_circ_0001451 has been investigated in colorectal cancer, it remains unclear about the relationship between Hsa_circ_0001451 and clear cell renal cell carcinoma (ccRCC). Our research aims to reveal the function of Hsa_circ_0001451 in the proliferation and development in ccRCC cells. Methods: The expression of Hsa_circ_0001451 in 52 pairs of ccRCC tissues and paraneoplastic tissues was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The correlation between Hsa_circ_0001451 and the clinicopathological features was evaluated using the chi-sequare test. Receiver operating characteristic (ROC) curve was built by SPSS to evaluate the diagnostic values. The effects of Hsa_circ_0001451 on ccRCC cells were determined via a MTT assay, clone formation assay, flow cytometry and Western blot analysis. Results: The expression of Hsa_circ_0001451 was significantly correlated with differentiation (P<0.05). The area under ROC curve of Hsa_circ_0001451 was 0.704 (P<0.05). Knockdown of Hsa_circ_0001451 significantly promoted tumor growth in vitro. Bioinformatics results also displayed that Hsa_circ_0001451 might be involved in the regulation of tumor progression. Conclusion: Taken together, our finding showed that Hsa_circ_0001451 might become a novel potential biomarker in the diagnosis of ccRCC and a potential novel target for the treatment of ccRCC.

[1]  Chawnshang Chang,et al.  Androgen receptor (AR) promotes clear cell renal cell carcinoma (ccRCC) migration and invasion via altering the circHIAT1/miR-195-5p/29a-3p/29c-3p/CDC42 signals. , 2017, Cancer letters.

[2]  B. Xiao,et al.  Circular RNA 0000096 affects cell growth and migration in gastric cancer , 2017, British Journal of Cancer.

[3]  Xiaoping Su,et al.  Circular RNA MTO 1 acts as the sponge of miR-9 to suppress hepatocellular carcinoma progression , 2017 .

[4]  Tao Sun,et al.  Regulatory Role of Circular RNAs and Neurological Disorders , 2017, Molecular Neurobiology.

[5]  Rogerio Margis,et al.  Circular RNAs are miRNA sponges and can be used as a new class of biomarker. , 2016, Journal of biotechnology.

[6]  R. Chen,et al.  Circular RNA has_circ_0067934 is upregulated in esophageal squamous cell carcinoma and promoted proliferation , 2016, Scientific Reports.

[7]  M. Mann,et al.  Circular non-coding RNA ANRIL modulates ribosomal RNA maturation and atherosclerosis in humans , 2016, Nature Communications.

[8]  Julia Salzman,et al.  Circular RNAs: analysis, expression and potential functions , 2016, Development.

[9]  Yan Li,et al.  Circular RNA profiling reveals an abundant circHIPK3 that regulates cell growth by sponging multiple miRNAs , 2016, Nature Communications.

[10]  Xin Zhang,et al.  Circular RNA Related to the Chondrocyte ECM Regulates MMP13 Expression by Functioning as a MiR-136 ‘Sponge’ in Human Cartilage Degradation , 2016, Scientific Reports.

[11]  Xubao Liu,et al.  Circular RNAs: a new frontier in the study of human diseases , 2016, Journal of Medical Genetics.

[12]  J. Larkin,et al.  SnapShot: Renal Cell Carcinoma , 2015, Cell.

[13]  Haimin Li,et al.  Circular RNA: A new star of noncoding RNAs. , 2015, Cancer letters.

[14]  Andreas W. Schreiber,et al.  The RNA Binding Protein Quaking Regulates Formation of circRNAs , 2015, Cell.

[15]  E. Schuman,et al.  Neural circular RNAs are derived from synaptic genes and regulated by development and plasticity , 2015, Nature Neuroscience.

[16]  E. Westhof,et al.  Biogenesis of Circular RNAs , 2014, Cell.

[17]  Yuchen Liu,et al.  Construction of circular miRNA sponges targeting miR-21 or miR-221 and demonstration of their excellent anticancer effects on malignant melanoma cells. , 2013, The international journal of biochemistry & cell biology.

[18]  Thomas Preiss,et al.  Circular RNAs: splicing's enigma variations , 2013, The EMBO journal.

[19]  J. Kjems,et al.  Natural RNA circles function as efficient microRNA sponges , 2013, Nature.

[20]  Charles Gawad,et al.  Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types , 2012, PloS one.

[21]  P. Tan,et al.  The Karakiewicz nomogram is the most useful clinical predictor for survival outcomes in patients with localized renal cell carcinoma , 2011, Cancer.

[22]  Brad T. Sherman,et al.  Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists , 2008, Nucleic acids research.

[23]  Thomas D. Schmittgen,et al.  Analyzing real-time PCR data by the comparative CT method , 2008, Nature Protocols.

[24]  P. Shannon,et al.  Cytoscape: a software environment for integrated models of biomolecular interaction networks. , 2003, Genome research.

[25]  H. Moch,et al.  Prognostic utility of the recently recommended histologic classification and revised TNM staging system of renal cell carcinoma , 2000, Cancer.

[26]  J L Warren,et al.  Rising incidence of renal cell cancer in the United States. , 1999, JAMA.

[27]  C. Cocquerelle,et al.  Mis‐splicing yields circular RNA molecules , 1993, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.