Application of deoxyribonucleic acid (DNA) polymorphisms to the analysis of DNA recovered from sperm.

Sperms, collected following sexual activity of volunteers, were processed to isolate high-molecular weight deoxyribonucleic acid (DNA). These DNA samples were digested with particular restriction endonucleases and analyzed with probes that recognize polymorphic DNA regions within the human genome. The pattern of restriction fragment length polymorphisms (RFLP) detected by this test is identical to that observed with DNA prepared from blood of the male sexual partner. Therefore, RFLP analysis can be used to exclude or to determine the probable identity of an assailant in rape cases.

[1]  M. Soules,et al.  The forensic laboratory evaluation of evidence in alleged rape. , 1978, American Journal of Obstetrics and Gynecology.

[2]  P Berg,et al.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. , 1977, Journal of molecular biology.

[3]  R. White,et al.  A highly polymorphic locus in human DNA. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[4]  R. Shaler,et al.  Analysis of restriction fragment length polymorphisms in deoxyribonucleic acid (DNA) recovered from dried bloodstains. , 1986, Journal of forensic sciences.

[5]  J. Chawla,et al.  Identification of semen in 500 patients seen because of rape. , 1977, American journal of clinical pathology.

[6]  G. Schumann,et al.  Prostatic acid phosphatase. Current assessment in vaginal fluid of alleged rape victims. , 1976, American journal of clinical pathology.

[7]  Y. Kan,et al.  ANTENATAL DIAGNOSIS OF SICKLE-CELL ANÆMIA BY D.N.A. ANALYSIS OF AMNIOTIC-FLUID CELLS , 1978, The Lancet.

[8]  P. Newall.  The Identification of HLA-A9 in Dried Stains of Blood and Body Secretions , 1981 .

[9]  Sensabaugh Gf.  Uses of polymorphic red cell enzymes in forensic science. , 1981 .

[10]  S. Latt,et al.  Regional localization on the human X of DNA segments cloned from flow sorted chromosomes. , 1982, Nucleic acids research.

[11]  A. Arnaiz-Villena,et al.  HLA typing of dried sperm. , 1984, Journal of forensic sciences.

[12]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[13]  M. Wigler,et al.  Sequence and structure of the coding region of the human H-ras-1 gene from T24 bladder carcinoma cells. , 1983, Journal of molecular and applied genetics.

[14]  Fowler Jc,et al.  Examination of the correlation of groupings in blood and semen. , 1985 .