DBC2 negatively regulates the proliferation of breast cancer cells and induces apoptosis

Objective To gain insight into the biological function of DBC2 in MDA-MB-435S breast cancer cell line in vitro.Methods Transient DBC2 over-expression in MDA-MB-435S cell line was generated by lipofectamine2000 transfecfion.MTT assay was performed to verify the function of growth inhibition of DBC2 over-expression.The effects of transient DBC2 over-expression was checked by flow cytometry.'rUNEL was used to evaluate the apoptosis induced by DBC2.Results The over-expression of DBC2 could significantly inhibit the proliferation of MDA-MB-435S cells and the percentage of inhibition was from 25.95% to 36.43%.Transient over-expression of DBC2 could also induce cell cycle G1 arrest (64.05% vs.71.72%).Moreover,TUNEL assay showed that the percentage of apoptosis was about 8%.Conclusion Transient over-expression of DBC2 in vitro could inhibit the growth of breast cancer cells,possibly through the mechanisms of inducing G_1 cell cycle arrest and apoptosis. Key words: Breast carcinoma; Tumor-suppressing gene; Proliferation; Cell cycle; Apoptosis