Distorted segregation of RFLP markers in regenerated plants derived from anther culture of an F1 hybrid of rice

To examine whether parental alleles were randomly transmitted from heterozygous donor plants to anther culture (AC)-derived plants, two AC-derived populations and one F2 population in rice were compared for 50 RFLP markers distributed on the rice chromosomes. Two populations which were developed through different anther culture methods (the ordinary method and the direct regeneration method) and the F2 population were produced from an F1 hybrid between distantly related cultivars of Nipponbare (japonica type) and Milyang 23 (indica type). RFLP analysis revealed that ten and eleven of the 50 markers in the two AC-derived populations showed distorted segregation ratios from the theoretical ratio of 1:1. Parental alleles were not randomly transmitted from the F1 plant to the AC-derived plants. Additionally, the segregation ratios of seven and six RFLP markers, respectively, were distorted both from the 1:1 ratios and from the observed ratios in the F2 population. The chromosomal regions involving these markers were on chromosomes 1, 3, 7, 10, 11 and 12. Some of the regions were different in the two AC-derived populations. Non-random assortment of parental alleles might be influenced by anther culture methods.

[1]  V. Lefebvre,et al.  Construction of an intraspecific integrated linkage map of pepper using molecular markers and doubled-haploid progenies. , 1995, Genome.

[2]  S. Lin,et al.  A 300 kilobase interval genetic map of rice including 883 expressed sequences , 1994, Nature Genetics.

[3]  S. Tanksley,et al.  Saturated molecular map of the rice genome based on an interspecific backcross population. , 1994, Genetics.

[4]  M. Saba-El-Leil,et al.  RFLP analyses and segregation of molecular markers in plants produced by in vitro anther culture, selfing, and reciprocal crosses of two lines of self-incompatible Solanum chacoense. , 1994, Genome.

[5]  M. Fujita,et al.  Breeding of Candidate Lines for Male Sterility-Maintainer by Anther Culture for Hybrid Wheat Production Using an Sv Type Cytoplasm and a 1BL-1RS Chromosome. , 1994 .

[6]  S. Bentolila,et al.  Comparative genetic analyses of F2 plants and anther culture derived plants of maize , 1992 .

[7]  M. Nakagawa,et al.  Linkage map of restriction fragment length polymorphism loci in rice. , 1991 .

[8]  S. Tanksley,et al.  Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) , 1991 .

[9]  Y. Sano,et al.  The genic nature of gamete eliminator in rice. , 1990, Genetics.

[10]  K. C. Reed,et al.  Rapid transfer of DNA from agarose gels to nylon membranes. , 1985, Nucleic acids research.

[11]  W. F. Thompson,et al.  Rapid isolation of high molecular weight plant DNA. , 1980, Nucleic acids research.

[12]  H. Oka.  Analysis of genes controlling f(1) sterility in rice by the use of isogenic lines. , 1974, Genetics.

[13]  M. Nakagahara.  Genetic Mechanism on the Distorted Segregation of Marker Genes belonging to the Eleventh Linkage Group in Cultivated Rice , 1972 .

[14]  C. Quimio,et al.  Diallel analysis of callus induction and green-plant regeneration in rice anther culture. , 1990 .

[15]  Y. Nakamura.  Direct induction of pollen plants in rice anther culture , 1985 .

[16]  F. Skoog,et al.  Organic Growth Factor Requirements of Tobacco Tissue Cultures , 1965 .