Chemical composition of ten essential oils from calophyllum inophyllum linn and their toxicity against artemia salina

Essential oils from ten different parts of Calophyllum inophyllum were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) to study their chemical compositions. The yields were between 0.219 and 0.506 %. A total of 102 compounds were identified in the ten C. inophyllum essential oils, which are mostly monoterpenes, sesquiterpenes and their oxygenated derivatives. The numbers and percentages of identified compounds varied in the different parts of the plant: leaf (71, 54.94%), leaf stalk (22, 79.55%), flower (25, 51.24%), seed (25, 89.39%), seed-coat (69, 73.80%) fruit-pulp (15, 46.10%), stem wood (55, 59.40%), stem bark (9, 69.38%) root wood (51, 58.73%), and root bark (24, 74.66%). High content of cymene, terpinene, and limonene in the oils may be responsible for the vast ethno-medicinal applications of the plant. Toxicity experiments show that the oils were fairly toxic. Each part after 24 hours of exposure against Artemia salina gave the following LC50 values in μg/mL: leaf (68.8740 μg/mL), leaf-stalk (102.5692 μg/mL), flower (114.4410 μg/mL), seed (132.2324 μg/mL), seed coat (137.1206 μg/mL), fruit-pulp (135.0350 μg/mL) stem wood (126.1410 μg/mL), stem bark (149.7237 μg/mL), root wood (110.6539 μg/mL) and root bark (110.6539 μg/mL). The chemical compositions and toxicity levels of these ten Calophyllum inophyllum essential oils are reported for the first time in literature.

[1]  Y. Jo,et al.  Inhibitory effects of calophyllum inophyllum extract on atopic dermatitis induced by DNCB in mouse , 2016 .

[2]  B. Umamaheswari,et al.  EFFECT OF ETHANOLIC EXTRACT OF CALOPHYLLUM INOPHYLLUM LEAVES ON OXIDATIVESTRESS COMPLICATIONS IN MOUSE MODEL , 2016 .

[3]  A. Afolayan,et al.  Antifungal activity and brine shrimp toxicity assessment of Bulbine abyssinica used in the folk medicine in the Eastern Cape Province, South Africa , 2016 .

[4]  Chemical Composition, Essential Oil Characterization and Antimicrobial Activity of Carum copticum , 2016 .

[5]  M. Islam,et al.  In vitro thrombolytic, anthelmintic, anti-oxidant and cytotoxic activity with phytochemical screening of methanolic extract of Xanthium indicum leaves , 2015 .

[6]  M. R. Haque,et al.  Cytotoxic, thrombolytic, membrane stabilizing and anti-oxidant activities of Hygrophila schulli , 2015 .

[7]  E. Restiawaty,et al.  The Potential of Nyamplung (Calophyllum inophyllum L.) Seed Oil as Biodiesel Feedstock: Effect of Seed Moisture Content and Particle Size on Oil Yield , 2015 .

[8]  R. M. Freitas,et al.  Evaluation of p-cymene, a natural antioxidant. , 2015 .

[9]  Marcelo de Almeida Pinheiro,et al.  Gastroprotective effect of alpha-pinene and its correlation with antiulcerogenic activity of essential oils obtained from Hyptis species , 2015, Pharmacognosy magazine.

[10]  D. Gelain,et al.  Antinociceptive Activity and Redox Profile of the Monoterpenes (+)-Camphene, p-Cymene, and Geranyl Acetate in Experimental Models , 2013, ISRN toxicology.

[11]  G. Flamini,et al.  Chemical constituents, toxicity and antimicrobial activities of the essential oil of the leaves of Tectona grandis , 2013 .

[12]  S. K. Awasthi,et al.  Antidyslipidemic and antioxidant activity of the constituents isolated from the leaves of Calophyllum inophyllum. , 2012, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[13]  N. Ashwath,et al.  Beauty Leaf (Calophyllum inophyllum L.), tree: a tree with great economic potential , 2012 .

[14]  S. Sahoo,et al.  FORMULATION AND EVALUATION OF HERBAL TABLET CONTAINING METHANOLIC EXTRACT OF CALOPHYLLUM INOPHYLLUM , 2012 .

[15]  Y. Lau,et al.  Acute Oral Toxicity and Brine Shrimp Lethality of Elaeis guineensis Jacq., (Oil Palm Leaf) Methanol Extract , 2010, Molecules.

[16]  G. Pastore,et al.  Antioxidant potential of aroma compounds obtained by limonene biotransformation of orange essential oil , 2009 .

[17]  G. Ee,et al.  Xanthones from Calophyllum Inophyllum , 2009 .

[18]  Francesco Castelli,et al.  Interaction of four monoterpenes contained in essential oils with model membranes: implications for their antibacterial activity. , 2007, Journal of agricultural and food chemistry.

[19]  A. Dweck,et al.  Tamanu (Calophyllum inophyllum) – the African, Asian, Polynesian and Pacific Panacea , 2002, International journal of cosmetic science.

[20]  J. G. Santos,et al.  Central effects of citral, myrcene and limonene, constituents of essential oil chemotypes from Lippia alba (Mill.) n.e. Brown. , 2002, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[21]  C. M. Silva,et al.  Anticonvulsant activity of essential oils and active principles from chemotypes of Lippia alba (Mill.) N.E. Brown. , 2000, Biological & pharmaceutical bulletin.

[22]  S. Sotheeswaran,et al.  Anti-HIV coumarins from Calophyllum seed oil. , 1998, Bioorganic & medicinal chemistry letters.

[23]  N. Oberlies,et al.  Cytotoxic and insecticidal constituents of the unripe fruit of Persea americana. , 1998, Journal of natural products.

[24]  S. Hughes,et al.  Specific inhibition of human immunodeficiency virus type 1 reverse transcriptase mediated by soulattrolide, a coumarin isolated from the latex of calophyllum teysmannii. , 1996, Journal of natural products.

[25]  M. Boyd,et al.  New pyranocoumarins isolated from Calophyllum lanigerum and Calophyllum teysmannii. , 1996, Journal of natural products.

[26]  H. Dharmaratne,et al.  Xanthones from root bark of Calophyllum thwaitesii , 1996 .

[27]  K. Gustafson,et al.  The calanolides, a novel HIV-inhibitory class of coumarin derivatives from the tropical rainforest tree, Calophyllum lanigerum. , 1992, Journal of medicinal chemistry.

[28]  H. Locksley,et al.  Xanthones in the heartwood of Calophyllum inophyllum: A geographical survey , 1971 .

[29]  A. R.,et al.  The Useful Plants of West Tropical Africa , 1938, Nature.