In vivo endomicroscopy using three-dimensional optical coherence tomography and Fourier domain mode locked lasers

We report an endoscopic optical coherence tomography (OCT) system based on a Fourier Domain Mode Locked (FDML) laser, a novel data acquisition (DAQ) system with optical frequency clocking, and a high-speed spiralscanning fiber probe. The system is capable of acquiring three-dimensional (3D) in vivo datasets at 100,000 axial lines/s and 50 frames/s, enabled by the high sweep rates of the FDML laser and the efficient data processing of the DAQ system. This high imaging rate allows densely-sampled 3D datasets to be acquired, giving a resolvable feature size of 9 &mgr;m x 20 &mgr;m x 7 &mgr;m (transverse x longitudinal x axial, XYZ). In vivo 3D endomicroscopy is demonstrated in the rabbit colon, where individual colonic crypts are clearly visualized and measured. With further improvements in DAQ technology, the imaging speed will be scalable to the hundreds of thousands of axial lines/s supported by FDML lasers.

[1]  Junji Kato,et al.  Aberrant Crypt Foci of the Colon as Precursors of Adenoma and Cancer , 1998 .

[2]  J. Fujimoto,et al.  Buffered Fourier domain mode locking: Unidirectional swept laser sources for optical coherence tomography imaging at 370,000 lines/s. , 2006, Optics letters.

[3]  J. Fujimoto,et al.  Fourier Domain Mode Locking (FDML): A new laser operating regime and applications for optical coherence tomography. , 2006, Optics express.

[4]  A. Fercher,et al.  Performance of fourier domain vs. time domain optical coherence tomography. , 2003, Optics express.

[5]  S. Yun,et al.  High-speed optical frequency-domain imaging. , 2003, Optics express.

[6]  Changhuei Yang,et al.  Sensitivity advantage of swept source and Fourier domain optical coherence tomography. , 2003, Optics express.

[7]  J. Fujimoto,et al.  Speckle reduction in optical coherence tomography images by use of a spatially adaptive wavelet filter. , 2004, Optics letters.

[8]  B. Bouma,et al.  Improved signal-to-noise ratio in spectral-domain compared with time-domain optical coherence tomography. , 2003, Optics letters.

[9]  J. Fujimoto,et al.  Phase-sensitive optical coherence tomography at up to 370,000 lines per second using buffered Fourier domain mode-locked lasers. , 2007, Optics letters.

[10]  John A. Evans,et al.  Comprehensive volumetric optical microscopy in vivo , 2006, Nature Medicine.

[11]  Douglas K Rex,et al.  Maximizing Detection of Adenomas and Cancers During Colonoscopy , 2006, The American Journal of Gastroenterology.