Separation of acinar cells from lactating mammary gland using velocity sedimentation in an isokinetic gradient of Ficoll in tissue culture medium.

This report describes a procedure for the purification of acinar cells from the disaggregated lactating mammary gland of the rat. Following disaggregation of the 15 days postpartum mammary gland, two qualitatively different kinds of cells which exhibited alkaline phosphatase activity were identified. These two populations of cells were separated in a previously described isokinetic gradient of Ficoll in tissue culture medium using a centrifugal force of 55g for 14 minutes. The larger of these two types of cells was found in a well-defined subpopulation of mammary cells, 97.4 +/- 0.8% of which lacked demonstrable adenosine triphosphatase activity by light microscopy. Ultrastructurally, these cells were identified as being almost exclusively acinar cells. The potential significance of this method is discussed.

[1]  T. Pretlow,et al.  Separation of lipase-positive cells from suspensions of pancreas cells in an isokinetic gradient of Ficoll in tissue culture medium. , 1973, The American journal of pathology.

[2]  T. Pretlow,et al.  Separation of lymphocytes from disaggregated mouse malignant neoplasms by sedimentation in gradients of ficoll in tissue culture medium. , 1973, Journal of immunology.

[3]  T. Pretlow,et al.  Separation of hepatocytes from suspensions of mouse liver cells using programmed gradient sedimentation in gradients of ficoll in tissue sulture medium. , 1973, Analytical biochemistry.

[4]  Pretlow Tg nd,et al.  Separation of spleen colony forming units (CEU-S) from mouse bone marrow cells using velocity sedimentation in an isokinetic gradient of Ficoll in tissue culture medium. , 1973, The American journal of pathology.

[5]  T. Pretlow,et al.  Pathogenesis of hodgkin's disease: Separation and culture of different kinds of cells from hodgkin's disease in a sterile isokinetic gradient of ficoll in tissue culture medium , 1973, Cancer.

[6]  T. Pretlow,et al.  A new method for separating lymphocytes and granulocytes from human peripheral blood using programmed gradient sedimentation in an isokinetic gradient. , 1973, Immunology.

[7]  P. DOROTHYR..  CELL CONTACTS IN T H E MOUSE MAMMARY GLAND I. Normal Gland in Postnatal Development and the Secretory Cycle , 1973 .

[8]  Y. J. Topper,et al.  Dynamics of insulin action on mammary epithelium. , 1972, Nature: New biology.

[9]  Y. J. Topper,et al.  Is prolactin mitogenic for mammary epithelium? , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[10]  T. Pretlow,et al.  Separation of ascites myeloma cells, lymphocytes and macrophages by zonal centrifugation on an isokinetic gradient. , 1972, The American journal of pathology.

[11]  T. Pretlow,et al.  Separation of beating cardiac myocytes from suspensions of heart cells. , 1972, The American journal of pathology.

[12]  Y. J. Topper,et al.  Hormone-dependent accumulation of rough endoplasmic reticulum in mouse mammary epithelial cells in vitro. , 1971, The Journal of biological chemistry.

[13]  Y. J. Topper,et al.  Insulin-sepharose and the dynamics of insulin action. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[14]  D. Bissell,et al.  MORPHOLOGY AND FUNCTION OF CELLS OF HUMAN EMBRYONIC LIVER IN MONOLAYER CULTURE , 1971, The Journal of cell biology.

[15]  T. Pretlow.  Estimation of experimental conditions that permit cell separations by velocity sedimentation on isokinetic gradients of Ficoll in tissue culture medium. , 1971, Analytical biochemistry.

[16]  E. Rivera,et al.  Differential actions of insulin on enzyme activities in mammary organ culture , 1971, Journal of cellular physiology.

[17]  T. Murad.  A proposed histochemical and electron microscopic classification of human breast cancer according to cell of origin , 1971, Cancer.

[18]  D. Pitelka,et al.  A SCANNING ELECTRON MICROSCOPE STUDY OF THE LACTATING MAMMARY GLAND , 1971, The Journal of cell biology.

[19]  R. W. Turkington.  Hormonal regulation of rapidly labeled ribonucleic acid in mammary cells in vitro. , 1970, The Journal of biological chemistry.

[20]  C. Daniel,et al.  FILAMENTS IN THE DIVISION FURROW OF MOUSE MAMMARY CELLS , 1970, The Journal of cell biology.

[21]  Y. J. Topper,et al.  SOME ULTRASTRUCTURAL EFFECTS OF INSULIN, HYDROCORTISONE, AND PROLACTIN ON MAMMARY GLAND EXPLANTS , 1970, The Journal of cell biology.

[22]  C. Boone,et al.  Separation of mammalian cells using programmed gradient sedimentation. , 1969, Experimental and molecular pathology.

[23]  H. T. Gagné,et al.  Characteristics of cells dissociated from mouse mammary glands. I. Method of separation and morphology of parenchymal cells from lactating glands. , 1969, Experimental cell research.

[24]  C. Boone,et al.  Centrifugation of Mammalian Cells on Gradients: A New Rotor , 1968, Science.

[25]  O. Stein,et al.  LIPID SYNTHESIS, INTRACELLULAR TRANSPORT, AND SECRETION : II. Electron Microscopic Radioautographic Study of the Mouse Lactating Mammary Gland , 1967 .

[26]  R. L. DeHann.  Regulation of spontaneous activity and growth of embryonic chick heart cells in tissue culture. , 1967, Developmental biology.

[27]  A. Desalu.  Correlation of localization of alkaline and acid phosphatases with morphological development of the rat kidney , 1966, The Anatomical record.

[28]  I. Harary,et al.  IN VITRO STUDIES ON SINGLE BEATING RAT HEART CELLS. VI. ELECTRON MICROSCOPIC STUDIES OF SINGLE CELLS. , 1964, Journal of ultrastructure research.

[29]  S. Malamed.  USE OF A MICROCENTRIFUGE FOR PREPARATION OF ISOLATED MITOCHONDRIA AND CELL SUSPENSIONS FOR ELECTRON MICROSCOPY , 1963, The Journal of cell biology.

[30]  I. Harary,et al.  In vitro studies on single beating rat heart cells. I. Growth and organization. , 1963, Experimental cell research.

[31]  K. Wakim.  Liver Function: A Symposium on Approaches to the Quantitative Description of Liver Function , 1961 .

[32]  E. Lasfargues.  Cultivation and behavior in vitro of the normal mammary epithelium of the adult mouse , 1957, The Anatomical record.

[33]  J. Gowen,et al.  ON THE MECHANISM OF MILK SECRETION , 1931, The Journal of general physiology.