High‐resolution tomographic diffractive microscopy of biological samples

The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolution. The authors now study its use for biological applications, and imaged several preparations with fluorescence confocal microscopy and tomographic diffractive microscopy. The results highlight some interesting features of this instrument, which should attract the interest of biologists for this new technique.

[1]  E. Wolf Three-dimensional structure determination of semi-transparent objects from holographic data , 1969 .

[2]  Yongkeun Park,et al.  Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum , 2008, Proceedings of the National Academy of Sciences.

[3]  S. Eykyn Microbiology , 1950, The Lancet.

[4]  O. Haeberlé,et al.  Tomographic diffractive microscopy of transparent samples , 2008 .

[5]  M. Debailleul,et al.  High resolution reflection tomographic diffractive microscopy , 2010 .

[6]  O. Haeberlé,et al.  Holographic microscopy and diffractive microtomography of transparent samples , 2008 .

[7]  V. Lauer New approach to optical diffraction tomography yielding a vector equation of diffraction tomography and a novel tomographic microscope , 2002, Journal of microscopy.

[8]  S. V. King,et al.  Quantitative phase microscopy through differential interference imaging. , 2008, Journal of biomedical optics.

[9]  C. Fang-Yen,et al.  Optical diffraction tomography for high resolution live cell imaging. , 2009, Optics express.

[10]  G. Barbastathis,et al.  Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging. , 2010, Optics letters.

[11]  O. Haeberlé,et al.  High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples. , 2009, Optics letters.

[12]  B. Lina,et al.  Impact of influenza A virus neuraminidase mutations on the stability, activity, and sensibility of the neuraminidase to neuraminidase inhibitors. , 2008, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.