Stem cell characteristics of human trabecular bone-derived cells.

Human trabecular bone-derived cells (HTBs) have been used for many years as osteoblast progenitors. In this study we tested whether HTBs have stem cell characteristics; that is, whether they are pluripotent and able to self-renew. We show that HTBs readily differentiate into osteoblasts, chondrocytes, and adipocytes if subjected to the appropriate differentiating conditions. Importantly, differentiation into these three lineages is maintained in single cell clones derived by limiting dilution, following expansion over more than 20 cumulative population doublings. We conclude that cultures of HTBs are equivalent to cultures of "mesenchymal stem cells" (MSCs) isolated from bone marrow.

[1]  K. Seuwen,et al.  Bone morphogenetic protein‐2 stimulates adipogenic differentiation of mesenchymal precursor cells in synergy with BRL 49653 (rosiglitazone) , 2000, FEBS letters.

[2]  M. Nuttall,et al.  Human Trabecular Bone Cells Are Able to Express Both Osteoblastic and Adipocytic Phenotype: Implications for Osteopenic Disorders , 1998, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[3]  Sottile,et al.  Multi-lineage potential of human mesenchymal stem cells following clonal expansion. , 2001, Journal of musculoskeletal & neuronal interactions.

[4]  I. Martin,et al.  Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. , 1997, Endocrinology.

[5]  J. Aubin,et al.  Long‐term effects of physiologic concentrations of dexamethasone on human bone‐derived cells , 1990, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[6]  K. Seuwen,et al.  A high-capacity screen for adipogenic differentiation. , 2001, Analytical biochemistry.

[7]  H. Lorenz,et al.  Multilineage cells from human adipose tissue: implications for cell-based therapies. , 2001, Tissue engineering.

[8]  A. Friedenstein.  Precursor cells of mechanocytes. , 1976, International review of cytology.

[9]  A. Friedenstein.  Stromal mechanisms of bone marrow: cloning in vitro and retransplantation in vivo. , 1980, Haematology and blood transfusion.

[10]  J. Wergedal,et al.  Characterization of Cells Isolated and Cultured from Human Bone , 1984, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[11]  M. Pittenger,et al.  Multilineage potential of adult human mesenchymal stem cells. , 1999, Science.

[12]  R Cancedda,et al.  Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model. , 2000, Journal of cell science.

[13]  A. Friedenstein,et al.  Stromal stem cells: marrow-derived osteogenic precursors. , 1988, Ciba Foundation symposium.

[14]  D. Prockop.  Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues , 1997, Science.

[15]  A I Caplan,et al.  In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. , 1998, Experimental cell research.

[16]  M. Gowen,et al.  Effects of bovine parathyroid hormone and 1,25‐dihydroxyvitamin D3 on the production of prostaglandins by cells derived from human bone , 1984, FEBS letters.

[17]  J. Lehmann,et al.  Peroxisome proliferator-activated receptor-gamma activation by thiazolidinediones induces adipogenesis in bone marrow stromal cells. , 1996, Molecular pharmacology.

[18]  S. Bruder,et al.  Osteogenic differentiation of purified, culture‐expanded human mesenchymal stem cells in vitro , 1997, Journal of cellular biochemistry.

[19]  E. Lotzová,et al.  Immunobiology of bone marrow transplantation. , 1978, Seminars in hematology.

[20]  J. Poser,et al.  Production of osteocalcin by human bone cells in vitro. Effects of 1,25(OH)2D3, 24,25(OH)2D3, parathyroid hormone, and glucocorticoids. , 1984, Metabolic bone disease & related research.

[21]  A. Friedenstein,et al.  Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. , 1968, Transplantation.

[22]  A. Friedenstein,et al.  THE DEVELOPMENT OF FIBROBLAST COLONIES IN MONOLAYER CULTURES OF GUINEA‐PIG BONE MARROW AND SPLEEN CELLS , 1970, Cell and tissue kinetics.