Evaluation of Escherichia coli Cell Response to Antibiotic Treatment by Use of Raman Spectroscopy with Laser Tweezers

ABSTRACT Laser tweezers Raman spectroscopy was used to detect the cellular response of Escherichia coli cells to penicillin G-streptomycin and cefazolin. Time-dependent intensity changes of several Raman peaks at 729, 1,245, and 1,660 cm−1 enabled untreated cells and cells treated with the different antibiotic drugs to be distinguished.

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