Polyunsaturated fatty acids stimulate phosphatidylcholine synthesis in PC 12 cells

The synthesis of phospholipids in mammalian cells is regr-rlated by the availability of three critical precursor pools: those of choline, cytidirre triphosphatc and diacylglycerol. Diacylglycerols containing polyunsaturated fatty acids (PUFAs) apparently are preferentially utilized for phosphatide synthesis. PUFAs are known to play an important role in the development and function of mammalian brains. We therefore str"rdied the effects of unsaturated, monounsaturated and polyunsaturated fatty acids on the overall rates of phospholipid biosynthesis in PCl2 rat plreoclrromocytorna cel ls. Docosahexaenoic acid (DHA,22:6n-3), e icosapentaenoic acid (EPA,20:5n-3) and arachidonic acid (AA,20:4n-6) al l significantly stirnulated the incorporation of raC-choline into total cellular phospholipids. In contrast, monounsaturated oleic acid (OA) and the saturated palrnitic (PA) and stearic (SA) acids did not have this effect. The action of DHA was concentration-dependent between 5 and 50 pM; it beoatne statistically significant by 3 h after DHA treatment and then increased over the ensuing 3 h. DHA was preferentially incorporated into phosphatidylethanolamine (PE) and phosphatidylserine (PS), while AA predominatcd in phosphatidylcholine (PC). (() 2007 Elscvicr B.V. Al l r ichts rescrvcd.

[1]  A. Svanborg,et al.  Plasma Total Lipid, Cholesterol, Triglycerides, Phospholipids and Free Fatty Acids in a Healthy Scandinavian Population , 2009 .

[2]  George E. Marzloff,et al.  Synaptic proteins and phospholipids are increased in gerbil brain by administering uridine plus docosahexaenoic acid orally , 2006, Brain Research.

[3]  B. Davletov,et al.  Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3 , 2006, Nature.

[4]  H. Lodish,et al.  Long-chain Acyl-CoA Synthetase 6 Preferentially Promotes DHA Metabolism* , 2005, Journal of Biological Chemistry.

[5]  G. Gerber,et al.  Biochemical Demonstration of the Involvement of Fatty Acyl-CoA Synthetase in Fatty Acid Translocation across the Plasma Membrane* , 2004, Journal of Biological Chemistry.

[6]  D. Bennett,et al.  Consumption of fish and n-3 fatty acids and risk of incident Alzheimer disease. , 2003, Archives of neurology.

[7]  Hee-Yong Kim,et al.  Effects of docosapentaenoic acid on neuronal apoptosis , 2003, Lipids.

[8]  W. Stremmel,et al.  A new concept of cellular uptake and intracellular trafficking of long-chain fatty acids , 2001, Lipids.

[9]  K. Michaelsen,et al.  The essentiality of long chain n-3 fatty acids in relation to development and function of the brain and retina. , 2001, Progress in lipid research.

[10]  A. Di Biase,et al.  Diet, Lipids and Brain Development , 2000, Developmental Neuroscience.

[11]  M. Tierney,et al.  Fatty acid analysis of blood plasma of patients with alzheimer's disease, other types of dementia, and cognitive impairment , 2000, Lipids.

[12]  A. Bonen,et al.  Acute Regulation of Fatty Acid Uptake Involves the Cellular Redistribution of Fatty Acid Translocase* , 2000, The Journal of Biological Chemistry.

[13]  R. Wurtman,et al.  Heterogeneous Long Chain Acyl-CoA Synthetases Control Distribution of Individual Fatty Acids in Newly-Formed Glycerolipids of Neuronal Cells Undergoing Neurite Outgrowth , 1999, Neurochemical Research.

[14]  R. Wurtman,et al.  Nerve growth factor stimulates diacylglycerol de novo synthesis and phosphatidylinositol hydrolysis in pheochromocytoma cells , 1998, Brain Research.

[15]  R. Wurtman,et al.  Control of membrane phosphatidylcholine biosynthesis by diacylglycerol levels in neuronal cells undergoing neurite outgrowth. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[16]  Tokuo T. Yamamoto,et al.  Molecular Characterization and Expression of Rat Acyl-CoA Synthetase 3* , 1996, The Journal of Biological Chemistry.

[17]  P. Grimaldi,et al.  Expression of the CD36 homolog (FAT) in fibroblast cells: effects on fatty acid transport. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[18]  R. Wurtman,et al.  Effect of Cytidine on Membrane Phospholipid Synthesis in Rat Striatal Slices , 1995, Journal of neurochemistry.

[19]  N. Bazan,et al.  Changing Fatty Acid Content of Growth Cone Lipids Prior to Synaptogenesis , 1992, Journal of neurochemistry.

[20]  R. Wurtman,et al.  Enhancement by Cytidine of Membrane Phospholipid Synthesis , 1992, Journal of neurochemistry.

[21]  Manuela Martínez.  Abnormal profiles of polyunsaturated fatty acids in the brain, liver, kidney and retina of patients with peroxisomal disorders , 1992, Brain Research.

[22]  L. Horrocks,et al.  Effects of free fatty acids on the enzymic synthesis of diacyl and ether types of choline and ethanolamine phosphoglycerides. , 1976, Journal of lipid research.

[23]  R. Sundler,et al.  Effect of different fatty acids on glycerolipid synthesis in isolated rat hepatocytes. , 1974, The Journal of biological chemistry.

[24]  R. E. Anderson,et al.  Lipids of ocular tissues. VII. Positional distribution of the fatty acids in the phospholipids of bovine retina rod outer segments. , 1971, Archives of biochemistry and biophysics.

[25]  B. Ames,et al.  Is docosahexaenoic acid, an n-3 long-chain polyunsaturated fatty acid, required for development of normal brain function? An overview of evidence from cognitive and behavioral tests in humans and animals. , 2005, The American journal of clinical nutrition.

[26]  N. Abumrad,et al.  Fatty acid transport across membranes: relevance to nutrition and metabolic pathology. , 2002, Annual review of nutrition.

[27]  S. Fliesler,et al.  Chemistry and metabolism of lipids in the vertebrate retina. , 1983, Progress in lipid research.

[28]  A. J. Clifford,et al.  BIOCHIMICA ET BIOPHYSICA ACTA , 2022 .