TSC22D4 is a molecular output of hepatic wasting metabolism

In mammals, proper storage and distribution of lipids in and between tissues is essential for the maintenance of energy homeostasis. Here, we show that tumour growth triggers hepatic metabolic dysfunction as part of the cancer cachectic phenotype, particularly by reduced hepatic very‐low‐density‐lipoprotein (VLDL) secretion and hypobetalipoproteinemia. As a molecular cachexia output pathway, hepatic levels of the transcription factor transforming growth factor beta 1‐stimulated clone (TSC) 22 D4 were increased in cancer cachexia. Mimicking high cachectic levels of TSC22D4 in healthy livers led to the inhibition of hepatic VLDL release and lipogenic genes, and diminished systemic VLDL levels under both normal and high fat dietary conditions. Liver‐specific ablation of TSC22D4 triggered hypertriglyceridemia through the induction of hepatic VLDL secretion. Furthermore, hepatic TSC22D4 expression levels were correlated with the degree of body weight loss and VLDL hypo‐secretion in cancer cachexia, and TSC22D4 deficiency rescued tumour cell‐induced metabolic dysfunction in hepatocytes. Therefore, hepatic TSC22D4 activity may represent a molecular rationale for peripheral energy deprivation in subjects with metabolic wasting diseases, including cancer cachexia.

[1]  E. Arenas Faculty Opinions recommendation of Cholesterol and bile acid metabolism are impaired in mice lacking the nuclear oxysterol receptor LXR alpha. , 2015 .

[2]  D. Glass,et al.  Cancer cachexia: mediators, signaling, and metabolic pathways. , 2012, Cell metabolism.

[3]  B. Stiles,et al.  Apolipoprotein B Secretion Is Regulated by Hepatic Triglyceride, and Not Insulin, in a Model of Increased Hepatic Insulin Signaling , 2012, Arteriosclerosis, thrombosis, and vascular biology.

[4]  H. Kume,et al.  Transforming growth factor‐β‐stimulated clone‐22 is a negative‐feedback regulator of Ras / Raf signaling: Implications for tumorigenesis , 2012, Cancer science.

[5]  M. Laakso,et al.  Expression of the splicing factor gene SFRS10 is reduced in human obesity and contributes to enhanced lipogenesis. , 2011, Cell metabolism.

[6]  J. Werner,et al.  Molecular control of systemic bile acid homeostasis by the liver glucocorticoid receptor. , 2011, Cell metabolism.

[7]  D. Peeper,et al.  Antagonistic TSC22D1 variants control BRAFE600‐induced senescence , 2011, The EMBO journal.

[8]  Richard G. Lee,et al.  Antisense oligonucleotide reduction of apoB-ameliorated atherosclerosis in LDL receptor-deficient mice[S] , 2011, Journal of Lipid Research.

[9]  W. Wahli,et al.  Hepatic deficiency in transcriptional cofactor TBL1 promotes liver steatosis and hypertriglyceridemia. , 2011, Cell metabolism.

[10]  D. Lacey,et al.  Reversal of Cancer Cachexia and Muscle Wasting by ActRIIB Antagonism Leads to Prolonged Survival , 2010, Cell.

[11]  E. Bruera,et al.  Loss of adipose tissue and plasma phospholipids: relationship to survival in advanced cancer patients. , 2010, Clinical nutrition.

[12]  E. Hafen,et al.  Madm (Mlf1 adapter molecule) cooperates with Bunched A to promote growth in Drosophila , 2010, Journal of biology.

[13]  M. Montminy,et al.  TORC2 regulates hepatic insulin signaling via a mammalian phosphatidic acid phosphatase, LIPIN1. , 2009, Cell metabolism.

[14]  J. Tegnér,et al.  ApoB100-LDL Acts as a Metabolic Signal from Liver to Peripheral Fat Causing Inhibition of Lipolysis in Adipocytes , 2008, PloS one.

[15]  S. Herzig,et al.  Nuclear receptor cofactor receptor interacting protein 140 controls hepatic triglyceride metabolism during wasting in mice , 2008, Hepatology.

[16]  J. Girard,et al.  Contribution of de novo fatty acid synthesis to hepatic steatosis and insulin resistance: lessons from genetically engineered mice. , 2008, The Journal of clinical investigation.

[17]  A. Goldberg,et al.  FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells. , 2007, Cell metabolism.

[18]  K. V. van Dijk,et al.  Angptl4 Upregulates Cholesterol Synthesis in Liver via Inhibition of LPL- and HL-Dependent Hepatic Cholesterol Uptake , 2007, Arteriosclerosis, thrombosis, and vascular biology.

[19]  J. Wojtaszewski,et al.  Effect of endurance exercise training on Ca2+–calmodulin‐dependent protein kinase II expression and signalling in skeletal muscle of humans , 2007, The Journal of physiology.

[20]  J. Auwerx,et al.  Transcriptional coregulators in the control of energy homeostasis. , 2007, Trends in cell biology.

[21]  D. Kültz,et al.  Specific TSC22 domain transcripts are hypertonically induced and alternatively spliced to protect mouse kidney cells during osmotic stress , 2007, The FEBS journal.

[22]  S. Reed,et al.  Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors. , 2006, Journal of virological methods.

[23]  S. Dooley,et al.  Primary mouse hepatocytes for systems biology approaches: a standardized in vitro system for modelling of signal transduction pathways. , 2006, Systems biology.

[24]  R. E. Pitas,et al.  Mice fed a lipogenic methionine-choline-deficient diet develop hypermetabolism coincident with hepatic suppression of SCD-1 s⃞ Published, JLR Papers in Press, July 8, 2006. , 2006, Journal of Lipid Research.

[25]  W. Wahli,et al.  The Fasting-induced Adipose Factor/Angiopoietin-like Protein 4 Is Physically Associated with Lipoproteins and Governs Plasma Lipid Levels and Adiposity* , 2006, Journal of Biological Chemistry.

[26]  Helmut Friess,et al.  Role of Mononuclear Cells and Inflammatory Cytokines in Pancreatic Cancer-Related Cachexia , 2005, Clinical Cancer Research.

[27]  Marco Sandri,et al.  Foxo Transcription Factors Induce the Atrophy-Related Ubiquitin Ligase Atrogin-1 and Cause Skeletal Muscle Atrophy , 2004, Cell.

[28]  Ianessa Morantte,et al.  CREB controls hepatic lipid metabolism through nuclear hormone receptor PPAR-γ , 2003, Nature.

[29]  T. Willson,et al.  Peroxisome proliferator-activated receptor gamma-mediated differentiation: a mutation in colon cancer cells reveals divergent and cell type-specific mechanisms. , 2003, The Journal of biological chemistry.

[30]  X. Deng,et al.  Increased hepatic VLDL secretion, lipogenesis, and SREBP-1 expression in the corpulent JCR:LA-cp rat. , 2001, Journal of lipid research.

[31]  H. Westphal,et al.  Expression screening for Lhx3 downstream genes identifies Thg-1pit as a novel mouse gene involved in pituitary development. , 2001, Gene.

[32]  Marc Montminy,et al.  CREB regulates hepatic gluconeogenesis through the coactivator PGC-1 , 2001, Nature.

[33]  J. den Hertog,et al.  Transforming growth factor-beta-stimulated clone-22 is a member of a family of leucine zipper proteins that can homo- and heterodimerize and has transcriptional repressor activity. , 1999, The Journal of biological chemistry.

[34]  R. Hammer,et al.  Cholesterol and Bile Acid Metabolism Are Impaired in Mice Lacking the Nuclear Oxysterol Receptor LXRα , 1998, Cell.

[35]  R. Curi,et al.  Studies on the lipid metabolism of Walker 256 tumour‐bearing rats during the development of cancer cachexia , 1996, Biochemistry and molecular biology international.

[36]  M. Bennett,et al.  The natural history of nonalcoholic fatty liver: A follow‐up study , 1995, Hepatology.

[37]  A. Hofman,et al.  Postprandial lipoprotein metabolism in normolipidemic men with and without coronary artery disease. , 1991, Arteriosclerosis and thrombosis : a journal of vascular biology.

[38]  H. Ishitsuka,et al.  Experimental cancer cachexia induced by transplantable colon 26 adenocarcinoma in mice. , 1990, Cancer research.

[39]  G. Heldmaier,et al.  Short photoperiod and cold activate brown fat lipoprotein lipase in the Djungarian hamster. , 1989, The American journal of physiology.

[40]  Joseph R. Bertino,et al.  Prognostic effect of weight loss prior to chemotherapy in cancer patients. Eastern Cooperative Oncology Group. , 1980, The American journal of medicine.

[41]  P. Paul,et al.  Altered glucose metabolism in metastatic carcinoma. , 1975, Cancer research.

[42]  C. E. West,et al.  Separation of plasma lipoproteins by density-gradient ultracentrifugation. , 1975, Analytical biochemistry.

[43]  R. Kaaks,et al.  Diabetes mellitus type 2 - an independent risk factor for cancer? , 2010, Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association.

[44]  H. Friess,et al.  Liver macrophages contribute to pancreatic cancer-related cachexia. , 2009, Oncology reports.

[45]  K. Fearon,et al.  Cancer cachexia. , 1999, Surgical oncology.

[46]  J. Bülow,et al.  Inter-relationships between single carbon units' metabolism and resting energy expenditure in weight-losing patients with small cell lung cancer. Effects of methionine supply and chemotherapy. , 1994, European Journal of Cancer.