Mechanisms of the growth inhibitory effects of the isoflavonoid biochanin A on LNCaP cells and xenografts *

Isoflavones inhibit the growth of some types of tumor cells, including prostate adenocarcinoma. This study used LNCaP cells and xenografts to investigate the mechanisms of the antiproliferative effects of biochanin A, a major isoflavone present in red clover but not soy‐derived products.

[1]  G. Hudes,et al.  Modulation of endogenous β-tubulin isotype expression as a result of human βIII cDNA transfection into prostate carcinoma cells , 2001, British Journal of Cancer.

[2]  J W Gray,et al.  Comprehensive genome sequence analysis of a breast cancer amplicon. , 2001, Genome research.

[3]  K. Setchell,et al.  Bioavailability of pure isoflavones in healthy humans and analysis of commercial soy isoflavone supplements. , 2001, The Journal of nutrition.

[4]  Ying-bin Wang,et al.  Differential Expression of a Stress-modulating Gene, BRE, in the Adrenal Gland, in Adrenal Neoplasia, and in Abnormal Adrenal Tissues , 2001, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[5]  K. Pienta,et al.  Inhibition of prostate specific antigen expression by genistein in prostate cancer cells. , 2000, International journal of oncology.

[6]  H Adlercreutz,et al.  Rye bran and soy protein delay growth and increase apoptosis of human LNCaP prostate adenocarcinoma in nude mice , 2000, The Prostate.

[7]  Yung-Hyun Choi,et al.  p53‐independent Induction of p21 (WAF1/CIP1), Reduction of Cyclin B1 and G2/M Arrest by the Isoflavone Genistein in Human Prostate Carcinoma Cells , 2000, Japanese journal of cancer research : Gann.

[8]  P. Bartels,et al.  Prostate cancer prevention: review of target populations, pathological biomarkers, and chemopreventive agents. , 1999, Journal of clinical pathology.

[9]  E Mahlamäki,et al.  Hormone therapy failure in human prostate cancer: analysis by complementary DNA and tissue microarrays. , 1999, Journal of the National Cancer Institute.

[10]  T. Hunter,et al.  The tyrosine kinase negative regulator c-Cbl as a RING-type, E2-dependent ubiquitin-protein ligase. , 1999, Science.

[11]  G. Blackburn,et al.  Soybean phytochemicals inhibit the growth of transplantable human prostate carcinoma and tumor angiogenesis in mice. , 1999, The Journal of nutrition.

[12]  Sierra Ee,et al.  Recent advances in the understanding of the mechanism of membrane transport of folates and antifolates. , 1999, Seminars in oncology.

[13]  G. Miller,et al.  Molecular genetics and epidemiology of prostate carcinoma. , 1999, Endocrine reviews.

[14]  K. Setchell,et al.  Phytoestrogens: the biochemistry, physiology, and implications for human health of soy isoflavones. , 1998, The American journal of clinical nutrition.

[15]  J. Kavanagh,et al.  Growth inhibition of prostate cell lines in vitro by phyto-oestrogens. , 1998, British journal of urology.

[16]  K. Hande.  Clinical applications of anticancer drugs targeted to topoisomerase II. , 1998, Biochimica et biophysica acta.

[17]  S. Weitman,et al.  The folate receptor as a potential therapeutic anticancer target , 1998, Investigational New Drugs.

[18]  J. Phang,et al.  Increased UDP-glucuronosyltransferase activity and decreased prostate specific antigen production by biochanin A in prostate cancer cells. , 1998, Cancer research.

[19]  T. Sugimura,et al.  Effects of soybean isoflavones on cell growth and apoptosis of the human prostatic cancer cell line LNCaP. , 1998, Japanese journal of clinical oncology.

[20]  S. Taneja,et al.  Phytoestrogens and prostate cancer: possible preventive role , 1998, The Medical journal of Australia.

[21]  Z. Shao,et al.  Genistein inhibits proliferation similarly in estrogen receptor‐positive and negative human breast carcinoma cell lines characterized by P21WAF1/CIP1 induction, G2/M arrest, and apoptosis , 1998, Journal of cellular biochemistry.

[22]  K. Dohi,et al.  Chemoprevention of N‐Nitroso‐N‐methylurea‐induced Rat Mammary Carcinogenesis by Soy Foods or Biochanin A , 1998, Japanese journal of cancer research : Gann.

[23]  R. Hoffman,et al.  Genistein inhibits the growth of human‐patient BPH and prostate cancer in histoculture , 1998, The Prostate.

[24]  G. Hudes,et al.  Cloning and sequencing of human betaIII-tubulin cDNA: induction of betaIII isotype in human prostate carcinoma cells by acute exposure to antimicrotubule agents. , 1998, Biochimica et biophysica acta.

[25]  W. Mazur,et al.  Phyto-oestrogens and Western diseases. , 1997, Annals of medicine.

[26]  E. Kyle,et al.  Genistein-induced apoptosis of prostate cancer cells is preceded by a specific decrease in focal adhesion kinase activity. , 1997, Molecular pharmacology.

[27]  L. Frati,et al.  Infection of human T lymphoid cells by human herpesvirus 6 is blocked by two unrelated protein tyrosine kinase inhibitors, biochanin A and herbimycin. , 1996, AIDS research and human retroviruses.

[28]  C. Falany,et al.  The role of metabolism in mammary epithelial cell growth inhibition by the isoflavones genistein and biochanin A. , 1996, Carcinogenesis.

[29]  S. Lieberman.  Are the differences between estradiol and other estrogens, naturally occurring or synthetic, merely semantical? , 1996, The Journal of clinical endocrinology and metabolism.

[30]  R. Vierstra,et al.  Identification of a Family of Closely Related Human Ubiquitin Conjugating Enzymes (*) , 1995, The Journal of Biological Chemistry.

[31]  K. Griffiths,et al.  Inhibition of 5 alpha-reductase in genital skin fibroblasts and prostate tissue by dietary lignans and isoflavonoids. , 1995, The Journal of endocrinology.

[32]  H. Adlercreutz.  Phytoestrogens: epidemiology and a possible role in cancer protection. , 1995, Environmental health perspectives.

[33]  H. Kizaki,et al.  Effects of protein tyrosine kinase inhibitors with different modes of action on topoisomerase activity and death of IL-2-dependent CTLL-2 cells. , 1995, Journal of biochemistry.

[34]  S. Barnes.  Effect of genistein on in vitro and in vivo models of cancer. , 1995, The Journal of nutrition.

[35]  F. Becker,et al.  Identification of a brain- and reproductive-organs-specific gene responsive to DNA damage and retinoic acid. , 1995, Biochemical and biophysical research communications.

[36]  R. Morton,et al.  Racial differences in adenocarcinoma of the prostate in North American men. , 1994, Urology.

[37]  E. Greenberg,et al.  Changes in prostate cancer incidence and treatment in USA , 1994, The Lancet.

[38]  K. Yanagihara,et al.  Antiproliferative effects of isoflavones on human cancer cell lines established from the gastrointestinal tract. , 1993, Cancer research.

[39]  M. Cazzola,et al.  Inhibitors of tyrosine phosphorylation induce apoptosis in human leukemic cell lines. , 1993, Leukemia.

[40]  R. Geahlen,et al.  Protein-tyrosine kinase inhibition: mechanism-based discovery of antitumor agents. , 1992, Journal of natural products.

[41]  M. Stearns,et al.  Prostate cancer: therapeutic, diagnostic, and basic studies. , 1992, Laboratory investigation; a journal of technical methods and pathology.

[42]  Y. Lee,et al.  Inhibitory effects of biochanin A on mouse lung tumor induced by benzo(a)pyrene. , 1991, Journal of Korean medical science.

[43]  W. Baird,et al.  Effects of biochanin A on metabolism, DNA binding and mutagenicity of benzo[a]pyrene in mammalian cell cultures. , 1991, Carcinogenesis.

[44]  S. Barnes,et al.  Genistein inhibition of the growth of human breast cancer cells: independence from estrogen receptors and the multi-drug resistance gene. , 1991, Biochemical and biophysical research communications.

[45]  W. Baird,et al.  Use of a mammalian cell culture benzo(a)pyrene metabolism assay for the detection of potential anticarcinogens from natural products: inhibition of metabolism by biochanin A, an isoflavone from Trifolium pratense L. , 1988, Cancer research.

[46]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[47]  E. Wynder,et al.  International comparisons of mortality rates for cancer of the breast, ovary, prostate, and colon, and per capita food consumption , 1986, Cancer.

[48]  G. Murphy,et al.  LNCaP model of human prostatic carcinoma. , 1983, Cancer research.

[49]  W. N. Burnette,et al.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. , 1981, Analytical biochemistry.

[50]  S. Shrivastav,et al.  An in vitro assay procedure to test chemotherapeutic drugs on cells from human solid tumors. , 1980, Cancer research.

[51]  J. E. Dunn.  Cancer epidemiology in populations of the United States--with emphasis on Hawaii and California--and Japan. , 1975, Cancer research.

[52]  W. Bruce,et al.  Expression profiling using cDNA microarrays. , 2003, Methods in molecular biology.

[53]  H. Lei,et al.  The potential of soybean foods as a chemoprevention approach for human urinary tract cancer. , 2000, Clinical cancer research : an official journal of the American Association for Cancer Research.

[54]  R. Elashoff,et al.  Decreased growth of human prostate LNCaP tumors in SCID mice fed a low-fat, soy protein diet with isoflavones. , 1999, Nutrition and cancer.

[55]  David B. Krizman,et al.  The genetics of cancer—a 3D model , 1999, Nature Genetics.

[56]  M. Bittner,et al.  Expression profiling using cDNA microarrays , 1999, Nature Genetics.

[57]  P. Goodfellow,et al.  DNA microarrays in drug discovery and development , 1999, Nature Genetics.

[58]  I. Goldman,et al.  Recent advances in the understanding of the mechanism of membrane transport of folates and antifolates. , 1999, Seminars in oncology.

[59]  F. Sarkar,et al.  Genistein-induced upregulation of p21WAF1, downregulation of cyclin B, and induction of apoptosis in prostate cancer cells. , 1998, Nutrition and cancer.

[60]  S. Barnes,et al.  Rationale for the use of genistein‐containing soy matrices in chemoprevention trials for breast and prostate cancer , 1995, Journal of cellular biochemistry. Supplement.

[61]  S. Keyse,et al.  Tyrosine kinase inhibition: an approach to drug development. , 1995, Human & experimental toxicology.

[62]  Friedewald Wt.  International comparisons of mortality rates. , 1994, Statistical bulletin.

[63]  Chung Lee,et al.  Growth of an androgen‐sensitive human prostate cancer cell line, LNCaP, in nude mice , 1993, The Prostate.

[64]  S. Barnes,et al.  Genistein and biochanin A inhibit the growth of human prostate cancer cells but not epidermal growth factor receptor tyrosine autophosphorylation , 1993, The Prostate.

[65]  H. Adlercreutz.  Western diet and Western diseases: some hormonal and biochemical mechanisms and associations. , 1990, Scandinavian journal of clinical and laboratory investigation. Supplementum.