Further studies of the kinetics of glucose utilization. I. A new method of data analysis.

ABSTRACT Evidence is presented to show that the extreme variation in rate constants for blood glucose disappearance reported by different laboratories is due to the use of an incorrect equation and/or an incorrect method of data analysis. It is demonstrated that graphic (semi-log) methods are too insensitive to be used to justify equations describing blood glucose disappearance. The correct equation is shown to be one of exponential form containing an added constant term which in the normal subject is significantly less than the fasting level of blood glucose. A new method of data analysis (slope analysis) is described which results in the same specific rate constant, K, regardless of the equation employed. This method is made practical by the introduction of a mechanical slope reader which eliminates excessive calculations.

[1]  Norton Nelson,et al.  A PHOTOMETRIC ADAPTATION OF THE SOMOGYI METHOD FOR THE DETERMINATION OF GLUCOSE , 1944 .

[2]  S. Soskin,et al.  A RELATIONSHIP BETWEEN THE BLOOD SUGAR LEVEL AND THE RATE OF SUGAR UTILIZATION, AFFECTING THE THEORIES OF DIABETES , 1937 .

[3]  P. Marks,et al.  The glucose metabolism of patients with malignant disease and of normal subjects as studied by means of an intravenous glucose tolerance test. , 1957, The Journal of clinical investigation.

[4]  M. Somogyi.  Studies of arteriovenous differences in blood sugar; effect of alimentary hyperglycemia on the rate of extrahepatic glucose assimilation. , 1948, The Journal of biological chemistry.

[5]  J. Herrick,et al.  THE MECHANISM OF REGULATION OF THE BLOOD SUGAR BY THE LIVER , 1938 .

[6]  A. G. Worthing,et al.  Treatment of experimental data , 1944 .

[7]  P. Bondy,et al.  STUDIES OF THE ROLE OF THE LIVER IN HUMAN CARBOHYDRATE METABOLISM BY THE VENOUS CATHETER TECHNIC. I. NORMAL SUBJECTS UNDER FASTING CONDITIONS AND FOLLOWING THE INJECTION OF GLUCOSE. , 1949, The Journal of clinical investigation.

[8]  S. Jokipii,et al.  Kinetics of elimination of glucose from the blood during and after a continuous intravenous injection. , 1954, The Journal of clinical investigation.

[9]  T. Witten,et al.  Studies on the kinetics of glucose utilization. , 1956, The Journal of clinical investigation.

[10]  P. Bastenie,et al.  Effect of Cortisone on Carbohydrate Metabolism Measured by the “Glucose Assimilation Coefficient” , 1954, Diabetes.

[11]  M. Goldstein,et al.  Influence of the liver on uptake of glucose by extrahepatic tissues. , 1954, The American journal of physiology.

[12]  E. Lozner,et al.  THE INTRAVENOUS GLUCOSE TOLERANCE TEST. , 1941, The Journal of clinical investigation.

[13]  D. S. Amatuzio,et al.  Interpretation of the rapid intravenous glucose tolerance test in normal individuals and in mild diabetes mellitus. , 1953, The Journal of clinical investigation.

[14]  N. Shock,et al.  Age differences in the intravenous glucose tolerance tests and the response to insulin. , 1957, The Journal of clinical investigation.