Allelic Diversity Revealed Through SSR Polymorphisms at the Locus Encoding HMG-CoA Reductase in Rubber (Hevea brasiliensis)

This study was carried out to define the extent of allelic variation of 3-hydroxy-3-methylglutaryl-CoA reductase gene (HMGR) in wild Hevea accessions, based on SSR polymorphisms existing at their 3’-untranslated regions (UTRs). Existence of two microsatellite alleles and their repeat compositions was demonstrated earlier in cultivated rubber clones. Both alleles contained perfect poly (AG) n repeats interrupted by a short sequence of 12 nucleotides and allelic variation at this microsatellite locus was the result of repeat length polymorphisms. In wild populations of rubber, nine microsatellite alleles (‘A’ to ‘I’) were identified at the HMGR locus revealing a wide allelic diversity compared to cultivated clones. Out

[1]  S. Knapp,et al.  The Sunflower High-oleic Mutant Ol Carries Variable Tandem Repeats of FAD2-1, a Seed-specific Oleoyl-phosphatidyl Choline Desaturase , 2006, Molecular Breeding.

[2]  M. Nazeer,et al.  Microsatellite variability and its use in the characterization of cultivated clones of Hevea brasiliensis , 2005 .

[3]  E. Nevo,et al.  Microsatellites within genes: structure, function, and evolution. , 2004, Molecular biology and evolution.

[4]  R. Varshney,et al.  Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) , 2003, Theoretical and Applied Genetics.

[5]  H. Corke,et al.  Microsatellites in starch-synthesizing genes in relation to starch physicochemical properties in waxy rice (Oryza sativa L.) , 2002, Theoretical and Applied Genetics.

[6]  M. Morgante,et al.  Microsatellites are preferentially associated with nonrepetitive DNA in plant genomes , 2002, Nature Genetics.

[7]  R. J. Henry,et al.  Analysis of SSRs derived from grape ESTs , 2000, Theoretical and Applied Genetics.

[8]  L. Lipovich,et al.  Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.) , 2000, Theoretical and Applied Genetics.

[9]  M. Chan,et al.  The 3' untranslated region of a rice alpha-amylase gene functions as a sugar-dependent mRNA stability determinant. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[10]  Y. A. Varghese,et al.  Evaluation of random amplified polymorphic DNA (RAPD) markers in Hevea brasiliensis , 1997 .

[11]  Yves Van de Peer,et al.  TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment , 1994, Comput. Appl. Biosci..

[12]  P. Besse,et al.  Genetic diversity among wild and cultivated populations of Hevea brasiliensis assessed by nuclear RFLP analysis , 1994, Theoretical and Applied Genetics.

[13]  Warren Dean,et al.  Brazil and the Struggle for Rubber: A Study in Environmental History , 1987 .

[14]  M. Nei.  Analysis of gene diversity in subdivided populations. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[15]  Stan C. Hokanson,et al.  Strawberry GenBank-derived and Genomic Simple Sequence Repeat (SSR) Markers and Their Utility with Strawberry, Blackberry, and Red and Black Raspberry , 2005 .

[16]  C. Neeraja,et al.  Characterization of tall landraces of rice (Oryza sativa L.) using gene-derived simple sequence repeats , 2005 .

[17]  A. Melchinger,et al.  Genetic diversity in European and Mediterranean faba bean germ plasm revealed by RAPD markers , 2004, Theoretical and Applied Genetics.

[18]  M. Nazeer,et al.  Identification of simple sequence repeats in rubber (Hevea brasiliensis) , 2004 .

[19]  Marc Seguin,et al.  Genetic diversity analysis of wild germplasm and cultivated clones of Hevea brasiliensis Muell. Arg. by using microsatellite markers , 2003 .

[20]  M. Rodier-Goud,et al.  A saturated genetic linkage map of rubber tree (Hevea spp.) based on RFLP, AFLP, microsatellite, and isozyme markers , 2000, Theoretical and Applied Genetics.

[21]  Rong‐Cai Yang,et al.  POPGENE, version 1.32: the user friendly software for population genetic analysis , 1999 .

[22]  A. McClung,et al.  Microsatellites and a single-nucleotide polymorphism differentiate apparentamylose classes in an extended pedigree of US rice germ plasm , 1997, Theoretical and Applied Genetics.

[23]  S. Atan,et al.  Recent advances in the development of molecular markers for Hevea studies , 1996 .

[24]  J. Doyle,et al.  Isolation of plant DNA from fresh tissue , 1990 .

[25]  M. Chevallier.  Genetic variability of Hevea brasiliensis germplasm using isozyme markers , 1988 .