Shear sensitivity in animal cell culture.

Over the past year, considerable progress has been made in understanding shear sensitivity in animal cell culture as a result of extensive theoretical and experimental work. Here we review this progress, paying special attention to the physical and biological mechanisms by which mechanical forces act upon cells, and the effects of such forces.

[1]  J. Frangos,et al.  Fluid shear stress as a mediator of osteoblast cyclic adenosine monophosphate production , 1990, Journal of cellular physiology.

[2]  J. Frangos,et al.  Shear‐induced platelet‐derived growth factor gene expression in human endothelial cells is mediated by protein kinase C , 1992, Journal of cellular physiology.

[3]  R. Cherry,et al.  Cell Death in the Thin Films of Bursting Bubbles , 1992, Biotechnology progress.

[4]  M. Kuchan,et al.  Shear stress regulates endothelin-1 release via protein kinase C and cGMP in cultured endothelial cells. , 1993, The American journal of physiology.

[5]  M. Goosen Large-scale insect cell culture. , 1992 .

[6]  E. Papoutsakis,et al.  Agitation induced cell injury in microcarrier cultures. Protective effect of viscosity is agitation intensity dependent: Experiments and modeling , 1992, Biotechnology and bioengineering.

[7]  D. Needham,et al.  A physical characterization of GAP A3 hybridoma cells: Morphology, geometry, and mechanical properties , 1991, Biotechnology and bioengineering.

[8]  J. Frangos,et al.  Fluid shear stress stimulates membrane phospholipid metabolism in cultured human endothelial cells. , 1992, Journal of vascular research.

[9]  E. Papoutsakis,et al.  Media additives for protecting freely suspended animal cells against agitation and aeration damage. , 1991, Trends in biotechnology.

[10]  L. Ju,et al.  Use of perfluorocarbon emulsions in cell culture. , 1992, BioTechniques.

[11]  E. Papoutsakis,et al.  Damaging agitation intensities increase DNA synthesis rate and alter cell‐cycle phase distributions of CHO cells , 1992, Biotechnology and bioengineering.

[12]  L. Fan,et al.  Microscopic Visualization of Insect Cell‐Bubble Interactions. I: Rising Bubbles, Air‐Medium Interface, and the Foam Layer , 1991, Biotechnology progress.

[13]  H. Caram,et al.  Optimal Design of the Tubular Microporous Membrane Aerator for Shear‐Sensitive Cell Cultures , 1992, Biotechnology progress.

[14]  J. Frangos,et al.  Flow‐induced prostacyclin production is mediated by a pertussis toxin‐sensitive G protein , 1992, FEBS letters.

[15]  N. Mohandas,et al.  Reversible deformation-dependent erythrocyte cation leak. Extreme sensitivity conferred by minimal peroxidation. , 1991, Biophysical journal.

[16]  F. Bavarian,et al.  Microscopic Visualization of Insect Cell‐Bubble Interactions. II: The Bubble Film and Bubble Rupture , 1991, Biotechnology progress.

[17]  K. Kawahara,et al.  Activation of calcium channel by shear-stress in cultured renal distal tubule cells. , 1992, Biochemical and biophysical research communications.

[18]  E. Papoutsakis,et al.  Fluid-mechanical damage of animal cells in bioreactors. , 1991, Trends in biotechnology.

[19]  R. Mutharasan,et al.  Effect of Serum on the Plasma Membrane Fluidity of Hybridomas: An Insight into Its Shear Protective Mechanism , 1992, Biotechnology progress.