Wide linear-range detecting high sensitivity cholesterol biosensors based on aspect-ratio controlled ZnO nanorods grown on silver electrodes

Abstract We have fabricated highly sensitive and wide linear-range detecting cholesterol biosensors based on zinc oxide nanorods (ZnO NRS). We report, for the first time, the use of aspect-ratio (AR) controlled ZnO NRs grown directly on silver (Ag) electrodes in solution at 90 °C for the fabrication of cholesterol biosensors. More immobilization of enzymes and faster electron conduction with larger AR ZnO NRs were obtained. The biosensor with AR = 60 ZnO NRs exhibits a high reproducible sensitivity of 74.10 μA/mM/cm2, a wide linear range up to 16.0 mM, and a fast response time less than 2 s, which is the best reported to date for cholesterol biosensors using ZnO nanostructures. Furthermore, the fabricated biosensors show an excellent anti-interference ability against electroactive species such as glucose, ascorbic acid, l -cysteine, and uric acid.

[1]  K. George,et al.  Electrical charge transport and dielectric response in ZnO nanotubes , 2011 .

[2]  W. März,et al.  Is lipoprotein(a) cholesterol a significant indicator of cardiovascular risk? , 2000, Clinical chemistry.

[3]  M. Mehrvar,et al.  Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors , 2004, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[4]  M. Rahman,et al.  Highly-sensitive cholesterol biosensor based on well-crystallized flower-shaped ZnO nanostructures. , 2009, Talanta.

[5]  Ahmad Umar,et al.  ZnO nanonails: synthesis and their application as glucose biosensor. , 2008, Journal of nanoscience and nanotechnology.

[6]  Jing-Juan Xu,et al.  Electrochemical Biosensors Based on Layer‐by‐Layer Assemblies , 2006 .

[7]  Asha Chaubey,et al.  Application of conducting polymers to biosensors. , 2002, Biosensors & bioelectronics.

[8]  Christian Soeller,et al.  Novel Conducting Polymers for DNA Sensing , 2007 .

[9]  M. Bertotti,et al.  Flow injection analysis of blood L-lactate by using a Prussian Blue-based biosensor as amperometric detector. , 2007, Analytical biochemistry.

[10]  Shen-Ming Chen,et al.  Simultaneous voltammetric detection of dopamine and ascorbic acid using didodecyldimethylammonium bromide (DDAB) film-modified electrodes , 2006 .

[11]  Magnus Willander,et al.  Chemically fashioned ZnO nanowalls and their potential application for potentiometric cholesterol biosensor , 2011 .

[12]  Bansi D Malhotra,et al.  Prospects of conducting polymers in biosensors. , 2006, Analytica chimica acta.

[13]  F. Ricci,et al.  Prussian Blue based screen printed biosensors with improved characteristics of long-term lifetime and pH stability. , 2003, Biosensors & bioelectronics.

[14]  Malika Ammam,et al.  In vivo biosensors , 2007, The FEBS journal.

[15]  Federica Valentini,et al.  Carbon nanotubes as electrode materials for the assembling of new electrochemical biosensors , 2004 .

[16]  O. Nur,et al.  White Electroluminescence Using ZnO Nanotubes/GaN Heterostructure Light-Emitting Diode , 2010, Nanoscale research letters.

[17]  Jianrong Chen,et al.  Nanotechnology and biosensors. , 2004, Biotechnology advances.

[18]  Yoon-Bo Shim,et al.  Electrochemical Sensors Based on Organic Conjugated Polymers , 2008, Sensors.

[19]  Shekhar Bhansali,et al.  Sensitive estimation of total cholesterol in blood using Au nanowires based micro-fluidic platform. , 2007, Biosensors & bioelectronics.

[20]  M. Vaseem,et al.  Ultra-sensitive cholesterol biosensor based on low-temperature grown ZnO nanoparticles , 2009 .

[21]  Y. Hahn.  Zinc oxide nanostructures and their applications , 2011 .

[22]  Bansi D Malhotra,et al.  Recent advances in cholesterol biosensor. , 2008, Biosensors & bioelectronics.

[23]  N. Jaffrezic‐Renault,et al.  Comparative study between organic and inorganic entrapment matrices for urease biosensor development , 2007 .

[24]  P. Solanki,et al.  Zinc oxide nanoparticles-chitosan composite film for cholesterol biosensor. , 2008, Analytica chimica acta.

[25]  B. D. Malhotra,et al.  Cholesterol biosensor based on rf sputtered zinc oxide nanoporous thin film , 2007 .