cDNA cloning of murine interleukin‐HP1: homology with human interleukin 6

Interleukin‐HP1 (HP1) is a murine T cell‐derived lymphokine, originally described as a growth factor for B cell hybridomas and plasmacytomas, that was recently shown to stimulate growth and differentiation of normal B and T lymphocytes. Here, we describe a cDNA for HP1 that was isolated from a library prepared using mRNA of a murine helper T cell clone activated with a clonotypic antibody. The cDNA, which hybridizes with a mRNA of ‐ 1300 bp, encodes a polypeptide consisting of 211 amino acids with a typical signal sequence of 24 residues followed by 187 amino acids, which form the mature protein (M, = 21710). No N‐glycosylation site but several potential O‐glycosylation sites were identified in the predicted sequence. Comparison of the cDNA sequence of HP1 with that of human interleukin 6 disclosed a homology of 65% at the DNA level and of 42% at the protein level with a maximum of 57% for the segment spanning residues 42–102 of mature HPI. Considering the functional homology that was previously established between these two proteins, we therefore propose that HP1 be renamed murine interleukin 6.

[1]  C. Auffray,et al.  Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. , 2005, European journal of biochemistry.

[2]  R. Simpson,et al.  Identification of an interleukin HP1-like plasmacytoma growth factor produced by L cells in response to viral infection. , 1987, Journal of immunology.

[3]  P. Lansdorp,et al.  Interferon beta 2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[4]  T. Hirano,et al.  Structure and expression of human B cell stimulatory factor‐2 (BSF‐2/IL‐6) gene. , 1987, The EMBO journal.

[5]  S. Rudikoff,et al.  Purification and NH2-terminal sequence of a plasmacytoma growth factor derived from the murine macrophage cell line P388D1. , 1987, Journal of immunology.

[6]  C. Richardson,et al.  DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[7]  W. Fiers,et al.  B cell growth modulating and differentiating activity of recombinant human 26‐kd protein (BSF‐2, HuIFN‐beta 2, HPGF). , 1987, The EMBO journal.

[8]  R. Simpson,et al.  Identification of the human 26-kD protein, interferon beta 2 (IFN-beta 2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor , 1987, The Journal of experimental medicine.

[9]  C. Uyttenhove,et al.  Interleukin-HP1, a T cell-derived hybridoma growth factor that supports the in vitro growth of murine plasmacytomas , 1987, The Journal of experimental medicine.

[10]  C. Uyttenhove,et al.  Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[11]  T. Taniguchi,et al.  Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin , 1986, Nature.

[12]  J H Korn,et al.  Structure and expression of cDNA and genes for human interferon‐beta‐2, a distinct species inducible by growth‐stimulatory cytokines. , 1986, The EMBO journal.

[13]  S. Nagata,et al.  Isolation and characterization of the cDNA for murine granulocyte colony-stimulating factor. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[14]  G. Volckaert,et al.  Structural analysis of the sequence coding for an inducible 26-kDa protein in human fibroblasts. , 1986, European journal of biochemistry.

[15]  R. Nordan,et al.  A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro. , 1986, Science.

[16]  R. Moritz,et al.  Rat epidermal growth factor: complete amino acid sequence. Homology with the corresponding murine and human proteins; isolation of a form truncated at both ends with full in vitro biological activity. , 1985, European journal of biochemistry.

[17]  D. Lipman,et al.  Rapid and sensitive protein similarity searches. , 1985, Science.

[18]  S. Henikoff.  Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. , 1984, Gene.

[19]  Y. Takahashi,et al.  Structure of human hemopexin: O-glycosyl and N-glycosyl sites and unusual clustering of tryptophan residues. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[20]  R. Lathe,et al.  New versatile cloning and sequencing vectors based on bacteriophage M13. , 1983, Gene.

[21]  B. Hoffman,et al.  A simple and very efficient method for generating cDNA libraries. , 1983, Gene.

[22]  J. Messing,et al.  Sequence analysis of zein cDNAs obtained by an efficient mRNA cloning method. , 1983, Nucleic acids research.

[23]  A. Feinberg,et al.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. , 1983, Analytical biochemistry.

[24]  R. Derynck,et al.  Secretory proteins induced in human fibroblasts under conditions used for the production of interferon beta. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[25]  F. Sanger,et al.  Determination of nucleotide sequences in DNA. , 1981, Bioscience reports.

[26]  J. Weissenbach,et al.  Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[27]  N. Proudfoot,et al.  3′ Non-coding region sequences in eukaryotic messenger RNA , 1976, Nature.

[28]  G. Opdenakker,et al.  Interleukin 1 and poly(rI) · poly(rC) induce production of a hybridoma growth factor by human fibroblasts , 1987, European journal of immunology.

[29]  S. Nagata,et al.  Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor , 1986, Nature.

[30]  M. O. Dayhoff,et al.  Establishing homologies in protein sequences. , 1983, Methods in enzymology.

[31]  D. Helfman,et al.  Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[32]  P Chambon,et al.  Organization and expression of eucaryotic split genes coding for proteins. , 1981, Annual review of biochemistry.