Synthesis of Ferrocene‐Branched Chitosan Derivatives: Redox Polysaccharides and their Application to Reagentless Enzyme‐Based Biosensors

Ferrocene-branched chitosan derivatives (CHIT-Fc) are synthesized by reductive N-alkylation of chitosan with ferrocenecarboxaldehyde. The structures of the products are determined by 1 H NMR and FT-IR spectra. CHIT-Fc is used as a functionalized matrix to immobilize GOD on glassy carbon electro- n des. Ferrocenyls in CHIT-Fc exhibit an excellent redox activity and establish efficient electrical communication between GOD and the electrodes for the oxidation of glucose. The development of a reagentless glucose biosensor is described.

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