Antimutagenic effects of lichen Pseudovernia furfuracea (L.) Zoph. extracts against the mutagenicity of aflatoxin B1 in vitro

The aim of this study was to investigate the effects of methanol, acetone, n-hexane and ether extracts obtained from Pseudovernia furfuracea on genotoxicity and total antioxidant capacity (TAC) in cultured human blood cells intoxicated with aflatoxin B 1 (AFB1). Sister chromatid exchange (SCE) and micronucleus (MN) tests were used for genotoxic influences estimation. In both the test systems, it was observed that P. furfuracea extracts suppressed the mutagenic effects of AFB1 due to the type of extracts added to the cultures. Furthermore, a significant reduction in plasma TAC was observed after AFB 1 treatment. Interestingly, the methanol and acetone extracts of the lichen recovered AFB1-induced TAC inhibition. The order of extracts of anti-genotoxicity efficacy against AFB1 was methanol, acetone, ether and n-hexane, respectively. In conclusion, P. furfuracea has been shown to modulate the adverse effects of AFB1 in human blood cells for the first time.

[1]  Mi-Kyung Lee,et al.  Anti-proliferative effects of Lethariella zahlbruckneri extracts in human HT-29 human colon cancer cells. , 2009, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[2]  P. Shanthi,et al.  Protective effect of Kalpaamruthaa in combating the oxidative stress posed by aflatoxin B1‐induced hepatocellular carcinoma with special reference to flavonoid structure–activity relationship , 2008, Liver international : official journal of the International Association for the Study of the Liver.

[3]  B. A. Tuylu,et al.  Determination of genotoxic, antigenotoxic and cytotoxic potential of the extract from lichen Cetraria aculeata (Schreb.) Fr. in vitro , 2008, Phytotherapy research : PTR.

[4]  F. Geyikoğlu,et al.  The protective roles of some lichen species on colloidal bismuth subcitrate genotoxicity , 2007, Toxicology and industrial health.

[5]  T. Massey,et al.  Elevation of 8-hydroxydeoxyguanosine in DNA from isolated mouse lung cells following in vivo treatment with aflatoxin B(1). , 2007, Toxicological sciences : an official journal of the Society of Toxicology.

[6]  M. Angeletti,et al.  Binding of aflatoxins to the 20S proteasome: effects on enzyme functionality and implications for oxidative stress and apoptosis , 2007, Biological chemistry.

[7]  V. Cardile,et al.  Pannarin inhibits cell growth and induces cell death in human prostate carcinoma DU-145 cells , 2006, Anti-cancer drugs.

[8]  R. Guevara-González,et al.  Relationship Among Antimutagenic, Antioxidant and Enzymatic Activities of Methanolic Extract from Common Beans (Phaseolus vulgaris L) , 2006, Plant foods for human nutrition.

[9]  Y. Bayir,et al.  Gastroprotective and antioxidant effects of usnic acid on indomethacin-induced gastric ulcer in rats. , 2006, Journal of ethnopharmacology.

[10]  F. Geyikoğlu,et al.  Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 , 2005 .

[11]  A. Henrici.  Lichens - an Illustrated Guide to the British and Irish Species , 2005 .

[12]  Y. Bayir,et al.  Effects of water extract of Usnea longissima on antioxidant enzyme activity and mucosal damage caused by indomethacin in rats. , 2005, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[13]  Nak-Ju Sung,et al.  Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions. , 2004, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[14]  O. Erel.  A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. , 2003, Clinical biochemistry.

[15]  H. Pawluk,et al.  The markers of oxidative stress and activity of the antioxidant system in the blood of elderly patients with essential arterial hypertension. , 2004, Cellular & molecular biology letters.

[16]  N. Sut,et al.  Sister chromatid exchanges in lymphocytes of nuclear medicine physicians. , 2003, Mutation research.

[17]  Y. Bayir,et al.  Anti-inflammatory and antiulcerogenic effects of the aqueous extract of Lobaria pulmonaria (L.) Hoffm. , 2003, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[18]  J. Boustie,et al.  Cytotoxic activity of some lichen extracts on murine and human cancer cell lines. , 2003, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[19]  G. Lóarca-Piña,et al.  Antioxidant activity in common beans (Phaseolus vulgaris L.). , 2002, Journal of agricultural and food chemistry.

[20]  M. Oktay,et al.  Determination of antioxidant activity of lichen Cetraria islandica (L) Ach. , 2002, Journal of ethnopharmacology.

[21]  A. Aslan,et al.  Lichen flora of the Murgul district, Artvin, Turkey , 2002 .

[22]  R. Verma,et al.  Ameliorative effect of vitamin E on aflatoxin-induced lipid peroxidation in the testis of mice. , 2001, Asian journal of andrology.

[23]  A. Srivastava,et al.  Long term effect of aflatoxin B1 on lipid peroxidation in rat liver and kidney: effect of picroliv and silymarin , 2001, Phytotherapy research : PTR.

[24]  I. Brodo,et al.  Lichens of North America , 2002 .

[25]  A. Wozniak,et al.  Influence of diethylenetriamine (DETA) and sodium nitroprusside (NaNP) on sister chromatid exchange frequency and cell kinetics in cultured human lymphocytes. , 2001, Journal of applied genetics.

[26]  M D Waters,et al.  IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety. , 2000, Mutation research.

[27]  A. Aslan.  LICHENS FROM THE REGIONS OF ARTVIN, ERZURUM, AND KARS (TURKEY) , 2000 .

[28]  S. Huneck.  The Significance of Lichens and Their Metabolites , 1999, Naturwissenschaften.

[29]  M. F. Souza,et al.  Inhibition by the Bioflavonoid Ternatin of Aflatoxin B1‐induced Lipid Peroxidation in Rat Liver , 1999, The Journal of pharmacy and pharmacology.

[30]  J. Groopman,et al.  The light at the end of the tunnel for chemical-specific biomarkers: daylight or headlight? , 1999, Carcinogenesis.

[31]  B. Dulger,et al.  Antimicrobial Activity of the Lichen Cetraria islandica (L.) Ach. , 1998 .

[32]  Y. Shen,et al.  Detection of elevated reactive oxygen species level in cultured rat hepatocytes treated with aflatoxin B1. , 1996, Free radical biology & medicine.

[33]  G. Yen,et al.  Relationship between antimutagenic activity and major components of various teas. , 1996, Mutagenesis.

[34]  Dr. Siegfried Huneck,et al.  Identification of Lichen Substances , 1996, Springer Berlin Heidelberg.

[35]  B. Proksa,et al.  Metabolites of Pseudevernia furfuracea (L.) Zopf. and their inhibition potential of proteolytic enzymes. , 1994, Die Pharmazie.

[36]  M Fenech,et al.  The cytokinesis-block micronucleus technique and its application to genotoxicity studies in human populations. , 1993, Environmental health perspectives.

[37]  O. W. Purvis.  The Lichen Flora of Great Britain and Ireland , 1993 .

[38]  M. Fenech,et al.  The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. , 1993, Mutation research.

[39]  S. Orrenius,et al.  Inhibition of DNA fragmentation in thymocytes and isolated thymocyte nuclei by agents that stimulate protein kinase C. , 1989, The Journal of biological chemistry.

[40]  C. Fraga,et al.  Damage to DNA concurrent with lipid peroxidation in rat liver slices. , 1988, The Biochemical journal.

[41]  R. San,et al.  Inhibitory effect of phenolic compounds on aflatoxin B1 metabolism and induced mutagenesis. , 1987, Mutation research.

[42]  S. Latt,et al.  Stimulated human phagocytes produce cytogenetic changes in cultured mammalian cells. , 1983, The New England journal of medicine.

[43]  H. Evans,et al.  Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests. , 1975, Mutation research.

[44]  S. Wolff,et al.  New Giemsa method for the differential staining of sister chromatids , 1974, Nature.

[45]  K. Vartia.  ANTIBIOTICS IN LICHENS , 1973 .

[46]  C. Culberson.  Chemical and botanical guide to lichen products , 1970 .

[47]  J. Poelt.  Bestimmungsschlüssel europäischer Flechten , 1969 .