A rapid detection method of Escherichia coli by surface enhanced Raman scattering

Conventional microbiological detection and enumeration methods are time-consuming, labor-intensive, and giving retrospective information. The objectives of the present work are to study the capability of surface enhanced Raman scattering (SERS) to detect Escherichia coli (E. coli) using the presented silver colloidal substrate. The obtained results showed that the adaptive iteratively reweighed Penalized Least Squares (airPLS) algorithm could effectively remove the fluorescent background from original Raman spectra, and Raman characteristic peaks of 558, 682, 726, 1128, 1210 and 1328 cm-1 could be observed stably in the baseline corrected SERS spectra of all studied bacterial concentrations. The detection limit of SERS could be determined to be as low as 0.73 log CFU/ml for E. coli with the prepared silver colloidal substrate. The quantitative prediction results using the intensity values of characteristic peaks were not good, with the correlation coefficients of calibration set and cross validation set of 0.99 and 0.64, respectively.

[1]  Bernhard Lendl,et al.  A New Method for Fast Preparation of Highly Surface-Enhanced Raman Scattering (SERS) Active Silver Colloids at Room Temperature by Reduction of Silver Nitrate with Hydroxylamine Hydrochloride , 2003 .

[2]  Yi-Zeng Liang,et al.  Baseline correction using adaptive iteratively reweighted penalized least squares. , 2010, The Analyst.

[3]  Royston Goodacre,et al.  Rapid and quantitative detection of the microbial spoilage of muscle foods: current status and future trends. , 2001 .

[4]  R. Dornhaus.  Spectral Properties of Pyrazine Adsorbed on Silver Electrodes and Cold Silver Films from Surface Enhanced Raman Scattering (SERS) , 1983 .

[5]  Ilaria Palchetti,et al.  Electroanalytical biosensors and their potential for food pathogen and toxin detection , 2008, Analytical and bioanalytical chemistry.

[6]  A. Savitzky,et al.  Smoothing and Differentiation of Data by Simplified Least Squares Procedures. , 1964 .

[7]  Reinhard Niessner,et al.  Optimized Surface-enhanced Raman Scattering (SERS) Colloids for the Characterization of Microorganisms , 2010, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[8]  R. Dornhaus,et al.  Spectral Properties of Pyrazine Adsorbed on Silver Electrodes and Cold Silver Films from Surface Enhanced Raman Scattering (SERS) , 1983 .

[9]  Steven R. Emory,et al.  Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman Scattering , 1997, Science.

[10]  Yi-Zeng Liang,et al.  Evaluation and prediction of the antioxidant activity of Epimedium from multi-wavelength chromatographic fingerprints and chemometrics , 2014 .

[11]  R. Goodacre,et al.  Discrimination of bacteria using surface-enhanced Raman spectroscopy. , 2004, Analytical chemistry.

[12]  R H Dainty,et al.  Chemical/biochemical detection of spoilage. , 1996, International journal of food microbiology.

[13]  Jürgen Popp,et al.  Classification of Raman spectra of single cells with autofluorescence suppression by wavelength modulated excitation , 2013 .

[14]  J. Sofos,et al.  Challenges to meat safety in the 21st century. , 2008, Meat science.

[15]  Bosoon Park,et al.  Surface enhanced Raman scattering (SERS) with biopolymer encapsulated silver nanosubstrates for rapid detection of foodborne pathogens. , 2013, International journal of food microbiology.

[16]  Thomas Brüning,et al.  It's in your blood: spectral biomarker candidates for urinary bladder cancer from automated FTIR spectroscopy , 2014, Journal of biophotonics.

[17]  S. Efrima,et al.  Surface-Enhanced Raman Spectroscopy as a Tool for Probing Specific Biochemical Components in Bacteria , 2004, Applied spectroscopy.

[18]  Atanu Sengupta,et al.  Surface-Enhanced Raman Spectroscopy of Bacteria and Pollen , 2005, Applied spectroscopy.

[19]  D. Kell,et al.  Rapid and Quantitative Detection of the Microbial Spoilage of Meat by Fourier Transform Infrared Spectroscopy and Machine Learning , 2002, Applied and Environmental Microbiology.

[20]  P. Hohenberg,et al.  Inhomogeneous electron gas , 1964 .

[21]  A. Otto,et al.  Surface enhanced Raman scattering (SERS), what do we know? , 1980 .

[22]  Igor K Lednev,et al.  Raman spectroscopy coupled with advanced statistics for differentiating menstrual and peripheral blood , 2014, Journal of biophotonics.