Adhesive fimbriae associated with porcine enterotoxigenic Escherichia coli of the O141 serotype

Enterotoxigenic Escherichia coli of the O141 serotype, isolated from piglets with postweaning coliform enteritis but producing none of the characterized adhesive fimbriae, was examined for fimbrial production by transmission electron microscopy. Two strains that produced numerous fimbriae were chosen for further characterization. The fimbriae were isolated and purified and had a subunit molecular weight of 17,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Using antiserum raised against this protein, we have shown it to be specific for the 17,000-molecular-weight band by immunoblotting and to be directed against the fimbriae by immunoelectron microscopy. These fimbriae were not produced when the bacteria were grown at 18 degrees C and did not show any mannose-resistant hemagglutination of the erythrocytes tested. We propose that these are a new type of adhesive fimbriae associated with porcine enterotoxigenic E. coli of the O141 serotype.

[1]  J. Vandekerckhove,et al.  Characterization and purification of the F17 adhesin on the surface of bovine enteropathogenic and septicemic Escherichia coli. , 1988, American journal of veterinary research.

[2]  M. Der Vartanian,et al.  CS31A, a new K88-related fimbrial antigen on bovine enterotoxigenic and septicemic Escherichia coli strains , 1988, Infection and immunity.

[3]  D. Hasse,et al.  Detection of enterotoxigenic Escherichia coli in piggeries in Victoria by DNA hybridisation using K88, K99, LT, ST1 and ST2 probes. , 1988, Veterinary microbiology.

[4]  D. Maneval,et al.  Purification, morphology, and genetics of a new fimbrial putative colonization factor of enterotoxigenic Escherichia coli O159:H4 , 1987, Infection and immunity.

[5]  S. Knutton,et al.  Identification of a new fimbrial structure in enterotoxigenic Escherichia coli (ETEC) serotype O148:H28 which adheres to human intestinal mucosa: a potentially new human ETEC colonization factor , 1987, Infection and immunity.

[6]  F. Mooi,et al.  The fimbrial adhesins of Escherichia coli. , 1986, Advances in microbial physiology.

[7]  G. Dougan,et al.  Expression of a cloned 987P adhesion-antigen fimbrial determinant in Escherichia coli K-12 strain HB101. , 1986, Gene.

[8]  M. Levine,et al.  Coli surface antigens 1 and 3 of colonization factor antigen II-positive enterotoxigenic Escherichia coli: morphology, purification, and immune responses in humans , 1984, Infection and immunity.

[9]  M. Levine,et al.  New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. , 1983, Microbiological reviews.

[10]  I. Ørskov,et al.  Serology of Escherichia coli Fimbriae , 1983 .

[11]  I. Orskov,et al.  Serology of Escherichia coli fimbriae. , 1983, Progress in allergy.

[12]  W. Gaastra,et al.  Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. , 1982, Microbiological reviews.

[13]  F. D. de Graaf,et al.  Production, purification, and characterization of the fimbrial adhesive antigen F41 isolated from calf enteropathogenic Escherichia coli strain B41M , 1982, Infection and immunity.

[14]  K. Altmann,et al.  A new method for the extraction and purification of K99 pili from enterotoxigenic Escherichia coli and their characterization. , 1982, The Biochemical journal.

[15]  Gyles Cl.  Limitations of the infant mouse test for Escherichia coli heat stable enterotoxin. , 1979 .

[16]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[17]  C. Gyles.  Limitations of the infant mouse test for Escherichia coli heat stable enterotoxin. , 1979, Canadian journal of comparative medicine : Revue canadienne de medecine comparee.

[18]  R. Isaacson,et al.  Colonization of porcine intestine by enterotoxigenic Escherichia coli: selection of piliated forms in vivo, adhesion of piliated forms to epithelial cells in vitro, and incidence of a pilus antigen among porcine enteropathogenic E. coli , 1977, Infection and immunity.

[19]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[20]  H. Smith,et al.  The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin. , 1970, Journal of medical microbiology.

[21]  C. Brinton.  The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria. , 1965, Transactions of the New York Academy of Sciences.

[22]  C. Brinton.  Non-Flagellar Appendages of Bacteria , 1959, Nature.