The density of myelinated fibres is related to the fascicle diameter in human superficial peroneal nerve Statistical study of 41 normal samples

The density of myelinated fibres in the superficial peroneal nerve was studied in 41 samples from 24 control human subjects. Photographic montages of the whole nerve fascicle were made from semithin and ultrathin transverse sections and used for a statistical analysis of sampling procedures, range of variations and relations between density and other variables. The results indicate that the spatial distribution of myelinated fibres within a nerve is often non-uniform. Therefore, it was not possible to define a statistically valid sampling system. The study of relations between variables shows the lack of any correlation between density and age and a considerable variation in the density. In contrast, there is a strong positive linear correlation between the surface area of the nerve fascicle and its content of myelinated fibres. That is, the fibre density of a given normal nerve is related to its diameter and can be predicted within a narrow range of error. We propose the term "derived density" for this value, and its application as a tool in the diagnosis of peripheral neuropathies is now being studied.

[1]  Daniel Schwartz,et al.  Methodes Statistiques a L'Usage des Medecins et des Biologistes , 1964 .

[2]  Y. Origuchi.  Quantitative histological study in the sural nerves of children , 1981, Brain and Development.

[3]  P. Dyck,et al.  Studies to improve fixation of human nerves , 1974 .

[4]  M. Rydmark,et al.  Changes in shape and size of cat spinal root myelinated nerve fibers during fixation and Vestopal-w embedding for electron microscopy. , 1982, Journal of ultrastructure research.

[5]  J. Stevens,et al.  Histometric evaluation of branches of peroneal nerve: technique for combined biopsy of muscle nerve and cutaneous nerve. , 1973, Brain research.

[6]  M. Swallow.  Fibre size and content of the anterior tibial nerve of the foot. , 1966, Journal of neurology, neurosurgery, and psychiatry.

[7]  R. Bronson,et al.  A contribution to the electron microscopic morphometric analysis of peripheral nerve , 1978, The Journal of comparative neurology.

[8]  H S GARVEN,et al.  The Nerve Fibre Populations of the Nerves of the Leg in Chronic Occlusive Arterial Disease in Man , 1962, Scottish medical journal.

[9]  R. Barrnett,et al.  GACH: a water-miscible, lipid-retaining embedding polymer for electron microscopy. , 1973, Journal of ultrastructure research.

[10]  J. Ochoa,et al.  The normal sural nerve in man. I. Ultrastructure and numbers of fibres and cells. , 1969, Acta neuropathologica.

[11]  A. Asbury,et al.  The pathology of peripheral nerve. , 1980, Muscle & nerve.

[12]  Studies to improve fixation of human nerves. 1. Effect of duration of glutaraldehyde fixation on peripheral nerve morphometry. , 1974, Journal of the neurological sciences.

[13]  D. Kirschner,et al.  Processing for electron microscopy alters membrane structure and packing in myelin. , 1980, Journal of ultrastructure research.

[14]  A new computer assisted method for morphological assessment of peripheral nerve regeneration: statistical analysis of spatial patterns of axons. , 1982, Hiroshima journal of medical sciences.

[15]  D. Harriman,et al.  The incidence of abnormality in control human peripheral nerves studied by single axon dissection. , 1970, Journal of neurology, neurosurgery, and psychiatry.

[16]  D. O'Sullivan,et al.  The fibre size and content of the radial and sural nerves. , 1968, Journal of neurology, neurosurgery, and psychiatry.

[17]  S. Ebbesson,et al.  A comparison of a systematic sampling method with complete random sampling for estimating total numbers of nerve fibers , 1972, The Anatomical record.