论文信息 - A microcomupter program for the computation of free energy of the secondary structure of RNA oligomers by Ninio's rules

A microcomupter program for the computation of free energy of the secondary structure of RNA oligomers by Ninio's rules

Abstract An interactive microcomputer program for the rapid computation of the free (ΔG) of the secondary structure of RNA molecules is presented. The program assigns free energies (in kcal/mol) to helices; bulge, internal, and multi-branch loops; hairpins; unparied, and G:C termini according to Ninio's rules, and displays a running total during computation. It is written in ‘Microsoft Basic’ and is apllicable to virtually any Basic system with no modification. The program enables rapid bench-top determinations of free energies attributable to secondary structure features of RNA oligomers, and eliminates both the tedium and risk of error associated with manual calculations.

Rita R. Colwell | M. T. MacDonell

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