Translational control of JunB, an AP‐1 transcription factor, in activated human endothelial cells

Stimulated endothelial cells (EC) assume an activated phenotype with pro‐inflammatory and prothrombotic features, requiring new gene and protein expression. New protein synthesis in activated EC is largely regulated by transcriptional events controlled by a variety of transcription factors. However, post‐transcriptional control of gene expression also influences phenotype and allows the cell to alter protein expression in a faster and more direct way than is typically possible with transcriptional mechanisms. We sought to demonstrate that post‐transcriptional control of gene expression occurs during EC activation. Using thrombin‐activated EC and a high‐throughput, microarray‐based approach, we identified a number of gene products that may be regulated through post‐transcriptional mechanisms, including the AP‐1 transcription factor JunB. Using polysome profiling, cytoplasts and other standard cell biologic techniques, JunB is shown to be regulated at a post‐transcriptional level during EC activation. In activated EC, the AP‐1 transcription factor JunB, is regulated on a post‐transcriptional level. Signal‐dependent control of translation may regulate transcription factor expression and therefore, subsequent transcriptional events in stimulated EC. J. Cell. Biochem. 114: 1519–1528, 2013. © 2013 Wiley Periodicals, Inc.

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