Abstract The transient behavior of natural circulation for boiling two-phase flow was investigated by simulating normal and abnormal start-up conditions to research the feasibility of natural circulation BWRs such as the SBWR. It was found that the instabilities, which are out-of-phase geysering, in-phase natural circulation oscillation and out-of-phase density wave instability, may occur during the start-up when the vapor generation rate is insufficient. In this paper, the mechanism of in-phase natural circulation oscillation induced by hydrostatic head fluctuation in steam separators, which has never been understood well enough, is experimentally clarified. Next, the effect of system pressure on the occurrences of the geysering and the natural circulation oscillation are investigated. Finally, from the results, a recommendation is provided to establish the rational start-up procedure and reactor configuration for natural circulation BWRs.