Quantification of in vitro retroviral replication using a one-tube real-time RT-PCR system incorporating direct RNA preparation.

[1]  R. Singh.  A solvent-free, rapid and simple virus RNA-release method for potato leafroll virus detection in aphids and plants by reverse transcription polymerase chain reaction. , 1999, Journal of virological methods.

[2]  D. Ho,et al.  Use of Real-Time PCR and Molecular Beacons To Detect Virus Replication in Human Immunodeficiency Virus Type 1-Infected Individuals on Prolonged Effective Antiretroviral Therapy , 1999, Journal of Virology.

[3]  J. Böni,et al.  Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan fluorogenic real-time detection system. , 1999, Journal of virological methods.

[4]  L. Heyndrickx,et al.  Isolation of HIV-1 RNA from plasma: evaluation of seven different methods for extraction (part two). , 1998, Journal of virological methods.

[5]  Smetsers Tf,et al.  Freezing of PCR master mixture retains full amplification activity and facilitates PCR standardisation for molecular diagnostics and real-time quantitative PCR. , 1998 .

[6]  C. Petropoulos,et al.  Advances in quantitative PCR technology: 5' nuclease assays. , 1998, Current opinion in biotechnology.

[7]  M. Estes,et al.  Use of heat release and an internal RNA standard control in reverse transcription-PCR detection of Norwalk virus from stool samples , 1997, Journal of clinical microbiology.

[8]  R. Klebe,et al.  RT-PCR without RNA isolation. , 1996, BioTechniques.

[9]  R. Novak,et al.  PCR-based detection of arboviral RNA from mosquitoes homogenized in detergent. , 1994, BioTechniques.

[10]  P. Luciw,et al.  Identification of the Rev transactivation and Rev-responsive elements of feline immunodeficiency virus , 1992, Journal of virology.

[11]  M. Salimans,et al.  Rapid and simple method for purification of nucleic acids , 1990, Journal of clinical microbiology.

[12]  H. Uemura,et al.  A new method for extracting DNA or RNA for polymerase chain reaction. , 1990, Journal of virological methods.

[13]  K. Neftel,et al.  Effects of beta-lactam antibiotics on proliferating eucaryotic cells , 1987, Antimicrobial Agents and Chemotherapy.

[14]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[15]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[16]  C. Dahle,et al.  Introduction to isolating RNA. , 1998, Methods in molecular biology.