Investigating pleuromutilin-producing Clitopilus species and related basidiomycetes.

Pleuromutilin is a broad-spectrum antibiotic that has been used in veterinary medicine for over 20 years, but is now gaining interest as a human therapeutic. The compound is a fungal secondary metabolite, but there is some degree of confusion within the literature concerning which species may produce pleuromutilin, with several differently named fungi reported to make the compound. Here, we describe a taxonomic survey of publicly available cultures known to produce pleuromutilin, and other similar species. The pleuromutilin production of these strains was assessed and a phylogenetic assessment was carried out based on the sequence of the nuclear rRNA internal transcribed spacer region. Eleven strains were confirmed as being pleuromutilin producers and all of these isolates appear to fall within a discrete clade of the genus Clitopilus. The phylogenetic analysis also highlights the need for a revision of the taxonomic status of these fungi.

[1]  C. Kelly,et al.  Clostridium difficile--more difficult than ever. , 2008, The New England journal of medicine.

[2]  B. Santamour.  As a superbug spreads, so does misinformation. , 2008, Hospitals & health networks.

[3]  A. Ohta,et al.  Bipolar incompatibility system of an ectomycorrhizal basidiomycete, Rhizopogon rubescens , 2008, Mycorrhiza.

[4]  H. Tomioka.  Development of new antituberculous agents based on new drug targets and structure–activity relationship , 2008, Expert opinion on drug discovery.

[5]  G. Moreno,et al.  Molecular phylogenetic studies show Omphalina giovanellae represents a new section of Clitopilus (Agaricomycetes). , 2007, Mycological research.

[6]  L. Dubreuil,et al.  In vitro activity against anaerobes of retapamulin, a new topical antibiotic for treatment of skin infections. , 2007, The Journal of antimicrobial chemotherapy.

[7]  D. Felmingham,et al.  P790 In vitro activity of retapamulin, a novel pleuromutilin, against Staphylococcus spp. (n = 1413) and Streptococcus pyogenes (n=503) from 26 European centres , 2007 .

[8]  E. Böttger,et al.  Visions & Reflections ( Minireview ) Antimicrobial agents targeting the ribosome : the issue of selectivity and toxicity lessons to be learned , 2007 .

[9]  Bernard R. Baum,et al.  Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components , 1997, Plant Molecular Biology Reporter.

[10]  D. Payne,et al.  Selection of Retapamulin, a Novel Pleuromutilin for Topical Use , 2006, Antimicrobial Agents and Chemotherapy.

[11]  D. J. Lodge,et al.  Major clades of Agaricales: a multilocus phylogenetic overview , 2006, Mycologia.

[12]  Ronald N. Jones,et al.  Activity of Retapamulin (SB-275833), a Novel Pleuromutilin, against Selected Resistant Gram-Positive Cocci , 2006, Antimicrobial Agents and Chemotherapy.

[13]  Mark S Butler,et al.  Natural products--the future scaffolds for novel antibiotics? , 2006, Biochemical pharmacology.

[14]  P. Mcghee,et al.  Single- and Multistep Resistance Selection Studies on the Activity of Retapamulin Compared to Other Agents against Staphylococcus aureus and Streptococcus pyogenes , 2006, Antimicrobial Agents and Chemotherapy.

[15]  J. Castaǹer,et al.  Retapamulin. Pleuromutilin antibiotic , 2006 .

[16]  A. Yonath,et al.  Inhibition of peptide bond formation by pleuromutilins: the structure of the 50S ribosomal subunit from Deinococcus radiodurans in complex with tiamulin , 2004, Molecular microbiology.

[17]  Sudhir Kumar,et al.  MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment , 2004, Briefings Bioinform..

[18]  A. Basilio,et al.  Screening of basidiomycetes for antimicrobial activities , 2000, Antonie van Leeuwenhoek.

[19]  J. Moncalvo,et al.  Phylogenetic analyses of the Lyophylleae (Agaricales, Basidiomycota) based on nuclear and mitochondrial rDNA sequences , 2002 .

[20]  D. Windsor,et al.  Treatment of resistant mycoplasma infection in immunocompromised patients with a new pleuromutilin antibiotic. , 2001, Journal of Infection.

[21]  Juan A. Blanco,et al.  Relationship between Monokaryotic Growth Rate and Mating Type in the Edible Basidiomycete Pleurotus ostreatus , 2001, Applied and Environmental Microbiology.

[22]  J. Moncalvo,et al.  Coprinus Pers. and the disposition of Coprinus species sensu lato , 2001 .

[23]  R. Baird,et al.  Rapid Mini-Preparation of Fungal DNA for PCR , 2000, Journal of Clinical Microbiology.

[24]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[25]  J. Zohar,et al.  Treatment of Resistant Ocd , 1996, European Psychiatry.

[26]  T. White.  Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics , 1990 .

[27]  K. R. Stewart,et al.  A method for generating protoplasts from Clitopilus pinsitus. , 1986, The Journal of antibiotics.

[28]  E. Brandl,et al.  Pleuromutilins. Fermentation, structure and biosynthesis. , 1976, The Journal of antibiotics.

[29]  D. A. Reid,et al.  Introduction to Fungi , 1970 .

[30]  W. J. Robbins,et al.  Antibiotic Substances from Basidiomycetes: IX. Drosophila Subtarata. (Batsch Ex Fr.) Quel. , 1952, Proceedings of the National Academy of Sciences of the United States of America.

[31]  W. J. Robbins,et al.  Antibiotic Substances From Basidiomycetes: VIII. Pleurotus Multilus (Fr.) Sacc. and Pleurotus Passeckerianus Pilat. , 1951, Proceedings of the National Academy of Sciences of the United States of America.

[32]  W. J. Robbins,et al.  Antibiotic Substances from Basidiomycetes: VI. Agrocybe Dura. , 1950, Proceedings of the National Academy of Sciences of the United States of America.

[33]  G. Harris,et al.  Investigation into the production of Bacteriostatic Substances by Fungi. VI. Examination of the Larger Basidiomycetes. , 1944 .